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Point‐of‐care fluorescence imaging reveals extent of bacterial load in diabetic foot ulcers

Elevated levels of bacteria, including biofilm, increase the risk of chronic wound infection and inhibit healing. Addressing asymptomatic high bacterial loads is challenged by a lack of clinical terminology and diagnostic tools. This post‐hoc multicenter clinical trial analysis of 138 diabetic foot...

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Autores principales: Armstrong, David G., Edmonds, Michael E., Serena, Thomas E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9885466/
https://www.ncbi.nlm.nih.gov/pubmed/36708275
http://dx.doi.org/10.1111/iwj.14080
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author Armstrong, David G.
Edmonds, Michael E.
Serena, Thomas E.
author_facet Armstrong, David G.
Edmonds, Michael E.
Serena, Thomas E.
author_sort Armstrong, David G.
collection PubMed
description Elevated levels of bacteria, including biofilm, increase the risk of chronic wound infection and inhibit healing. Addressing asymptomatic high bacterial loads is challenged by a lack of clinical terminology and diagnostic tools. This post‐hoc multicenter clinical trial analysis of 138 diabetic foot ulcers investigates fluorescence (FL)‐imaging role in detecting biofilm‐encased and planktonic bacteria in wounds at high loads. The sensitivity and specificity of clinical assessment and FL‐imaging were compared across bacterial loads of concern (10(4)–10(9) CFU/g). Quantitative tissue culture confirmed the total loads. Bacterial presence was confirmed in 131/138 ulcers. Of these, 93.9% had loads >10(4) CFU/g. In those wounds, symptoms of infection were largely absent and did not correlate with, or increase proportionately with, bacterial loads at any threshold. FL‐imaging increased sensitivity for the detection of bacteria across loads 10(4)–10(9) (P < .0001), peaking at 92.6% for >10(8) CFU/g. Imaging further showed that 84.2% of ulcers contained high loads in the periwound region. New terminology, chronic inhibitory bacterial load (CIBL), describes frequently asymptomatic, high bacterial loads in diabetic ulcers and periwound tissues, which require clinical intervention to prevent sequelae of infection. We anticipate this will spark a paradigm shift in assessment and management, enabling earlier intervention along the bacterial‐infection continuum and supporting improved wound outcomes.
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spelling pubmed-98854662023-02-01 Point‐of‐care fluorescence imaging reveals extent of bacterial load in diabetic foot ulcers Armstrong, David G. Edmonds, Michael E. Serena, Thomas E. Int Wound J Original Articles Elevated levels of bacteria, including biofilm, increase the risk of chronic wound infection and inhibit healing. Addressing asymptomatic high bacterial loads is challenged by a lack of clinical terminology and diagnostic tools. This post‐hoc multicenter clinical trial analysis of 138 diabetic foot ulcers investigates fluorescence (FL)‐imaging role in detecting biofilm‐encased and planktonic bacteria in wounds at high loads. The sensitivity and specificity of clinical assessment and FL‐imaging were compared across bacterial loads of concern (10(4)–10(9) CFU/g). Quantitative tissue culture confirmed the total loads. Bacterial presence was confirmed in 131/138 ulcers. Of these, 93.9% had loads >10(4) CFU/g. In those wounds, symptoms of infection were largely absent and did not correlate with, or increase proportionately with, bacterial loads at any threshold. FL‐imaging increased sensitivity for the detection of bacteria across loads 10(4)–10(9) (P < .0001), peaking at 92.6% for >10(8) CFU/g. Imaging further showed that 84.2% of ulcers contained high loads in the periwound region. New terminology, chronic inhibitory bacterial load (CIBL), describes frequently asymptomatic, high bacterial loads in diabetic ulcers and periwound tissues, which require clinical intervention to prevent sequelae of infection. We anticipate this will spark a paradigm shift in assessment and management, enabling earlier intervention along the bacterial‐infection continuum and supporting improved wound outcomes. Blackwell Publishing Ltd 2023-01-28 /pmc/articles/PMC9885466/ /pubmed/36708275 http://dx.doi.org/10.1111/iwj.14080 Text en © 2023 The Authors. International Wound Journal published by Medicalhelplines.com Inc (3M) and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Original Articles
Armstrong, David G.
Edmonds, Michael E.
Serena, Thomas E.
Point‐of‐care fluorescence imaging reveals extent of bacterial load in diabetic foot ulcers
title Point‐of‐care fluorescence imaging reveals extent of bacterial load in diabetic foot ulcers
title_full Point‐of‐care fluorescence imaging reveals extent of bacterial load in diabetic foot ulcers
title_fullStr Point‐of‐care fluorescence imaging reveals extent of bacterial load in diabetic foot ulcers
title_full_unstemmed Point‐of‐care fluorescence imaging reveals extent of bacterial load in diabetic foot ulcers
title_short Point‐of‐care fluorescence imaging reveals extent of bacterial load in diabetic foot ulcers
title_sort point‐of‐care fluorescence imaging reveals extent of bacterial load in diabetic foot ulcers
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9885466/
https://www.ncbi.nlm.nih.gov/pubmed/36708275
http://dx.doi.org/10.1111/iwj.14080
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