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Genetic diversity analysis and fingerprint construction of Korean pine (Pinus koraiensis) clonal seed orchard

Korean pine is a native tree species in Northeast China. In order to meet the needs of germplasm resource evaluation and molecular marker-assisted breeding of Korean pine, we collected Korean pine clones from 7 populations in Northeast China, analyzed the genetic diversity and genetic structure by S...

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Autores principales: Yan, Pingyu, Xie, Zixiong, Feng, Kele, Qiu, Xinyu, Zhang, Lei, Zhang, Hanguo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9886227/
https://www.ncbi.nlm.nih.gov/pubmed/36726668
http://dx.doi.org/10.3389/fpls.2022.1079571
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author Yan, Pingyu
Xie, Zixiong
Feng, Kele
Qiu, Xinyu
Zhang, Lei
Zhang, Hanguo
author_facet Yan, Pingyu
Xie, Zixiong
Feng, Kele
Qiu, Xinyu
Zhang, Lei
Zhang, Hanguo
author_sort Yan, Pingyu
collection PubMed
description Korean pine is a native tree species in Northeast China. In order to meet the needs of germplasm resource evaluation and molecular marker-assisted breeding of Korean pine, we collected Korean pine clones from 7 populations in Northeast China, analyzed the genetic diversity and genetic structure by SSR molecular marker technology and clustered them to revealed the inter- and intrapopulation differentiation characteristics of each clone. The fingerprint profiles of 161 Korean pine clones were also constructed. 77 alleles were detected for 11 markers, and 18 genotypes were identified on average for each marker. The PIC of the different markers ranged from 0.155-0.855, and the combination of PI and PIsibs for the 11 markers was 3.1 × 10(-8) and 1.14 × 10(-3), respectively. MANOVA showed that genetic variation existed mainly within populations, accounting for 98% of the total variation. The level of genetic differentiation among populations was low, with an average Nm between populations of 11.036. Genetic diversity is lower in the Lushuihe population and higher in the Tieli population. The 161 Korean pine clones were divided into 4 or 7 populations, and the 7 populations were not clearly distinguished from each other, with only the Lushuihe population showing partial differentiation. There is no significant correlation between the genetic distance of Korean pine populations and the geographical distance of their superior tree sources. This result can provide recommendations for future Korean pine breeding programs. The combination of 11 markers could completely distinguish 161 clones and establish the fingerprint. Genetic diversity of Korean pine clones from the 7 populations was abundant, and the genetic distances of individuals and populations were evenly dispersed. The fingerprint map can be used for the identification of Korean pine clones.
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spelling pubmed-98862272023-01-31 Genetic diversity analysis and fingerprint construction of Korean pine (Pinus koraiensis) clonal seed orchard Yan, Pingyu Xie, Zixiong Feng, Kele Qiu, Xinyu Zhang, Lei Zhang, Hanguo Front Plant Sci Plant Science Korean pine is a native tree species in Northeast China. In order to meet the needs of germplasm resource evaluation and molecular marker-assisted breeding of Korean pine, we collected Korean pine clones from 7 populations in Northeast China, analyzed the genetic diversity and genetic structure by SSR molecular marker technology and clustered them to revealed the inter- and intrapopulation differentiation characteristics of each clone. The fingerprint profiles of 161 Korean pine clones were also constructed. 77 alleles were detected for 11 markers, and 18 genotypes were identified on average for each marker. The PIC of the different markers ranged from 0.155-0.855, and the combination of PI and PIsibs for the 11 markers was 3.1 × 10(-8) and 1.14 × 10(-3), respectively. MANOVA showed that genetic variation existed mainly within populations, accounting for 98% of the total variation. The level of genetic differentiation among populations was low, with an average Nm between populations of 11.036. Genetic diversity is lower in the Lushuihe population and higher in the Tieli population. The 161 Korean pine clones were divided into 4 or 7 populations, and the 7 populations were not clearly distinguished from each other, with only the Lushuihe population showing partial differentiation. There is no significant correlation between the genetic distance of Korean pine populations and the geographical distance of their superior tree sources. This result can provide recommendations for future Korean pine breeding programs. The combination of 11 markers could completely distinguish 161 clones and establish the fingerprint. Genetic diversity of Korean pine clones from the 7 populations was abundant, and the genetic distances of individuals and populations were evenly dispersed. The fingerprint map can be used for the identification of Korean pine clones. Frontiers Media S.A. 2023-01-16 /pmc/articles/PMC9886227/ /pubmed/36726668 http://dx.doi.org/10.3389/fpls.2022.1079571 Text en Copyright © 2023 Yan, Xie, Feng, Qiu, Zhang and Zhang https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Yan, Pingyu
Xie, Zixiong
Feng, Kele
Qiu, Xinyu
Zhang, Lei
Zhang, Hanguo
Genetic diversity analysis and fingerprint construction of Korean pine (Pinus koraiensis) clonal seed orchard
title Genetic diversity analysis and fingerprint construction of Korean pine (Pinus koraiensis) clonal seed orchard
title_full Genetic diversity analysis and fingerprint construction of Korean pine (Pinus koraiensis) clonal seed orchard
title_fullStr Genetic diversity analysis and fingerprint construction of Korean pine (Pinus koraiensis) clonal seed orchard
title_full_unstemmed Genetic diversity analysis and fingerprint construction of Korean pine (Pinus koraiensis) clonal seed orchard
title_short Genetic diversity analysis and fingerprint construction of Korean pine (Pinus koraiensis) clonal seed orchard
title_sort genetic diversity analysis and fingerprint construction of korean pine (pinus koraiensis) clonal seed orchard
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9886227/
https://www.ncbi.nlm.nih.gov/pubmed/36726668
http://dx.doi.org/10.3389/fpls.2022.1079571
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