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Gardnerella subgroup dominant microbiomes are associated with divergent cervicovaginal immune responses in a longitudinal cohort of Kenyan women

Most cervicovaginal microbiome-immunology studies to date have relied on 16S rDNA microbial profiling which does not resolve the molecular subgroups of Gardnerella, believed to be central to the pathogenesis of bacterial vaginosis (BV) and subsequent risk of HIV acquisition. Here we used the cpn60 u...

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Autores principales: Shvartsman, Elinor, Perciani, Catia T., Richmond, Meika E. I., Russell, Justen N. H., Tough, Riley H., Vancuren, Sarah J., Hill, Janet E., KAVI-ICR, Jaoko, Walter, McKinnon, Lyle R., Sandstrom, Paul A., MacDonald, Kelly S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9886495/
https://www.ncbi.nlm.nih.gov/pubmed/36726972
http://dx.doi.org/10.3389/fimmu.2022.974195
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author Shvartsman, Elinor
Perciani, Catia T.
Richmond, Meika E. I.
Russell, Justen N. H.
Tough, Riley H.
Vancuren, Sarah J.
Hill, Janet E.
KAVI-ICR,
Jaoko, Walter
McKinnon, Lyle R.
Sandstrom, Paul A.
MacDonald, Kelly S.
author_facet Shvartsman, Elinor
Perciani, Catia T.
Richmond, Meika E. I.
Russell, Justen N. H.
Tough, Riley H.
Vancuren, Sarah J.
Hill, Janet E.
KAVI-ICR,
Jaoko, Walter
McKinnon, Lyle R.
Sandstrom, Paul A.
MacDonald, Kelly S.
author_sort Shvartsman, Elinor
collection PubMed
description Most cervicovaginal microbiome-immunology studies to date have relied on 16S rDNA microbial profiling which does not resolve the molecular subgroups of Gardnerella, believed to be central to the pathogenesis of bacterial vaginosis (BV) and subsequent risk of HIV acquisition. Here we used the cpn60 universal target which in addition to other microbial taxa, resolves four Gardnerella subgroups, for cervicovaginal microbial profiling in a longitudinal cohort of Kenyan women to examine associations with cellular and soluble markers of inflammation and HIV susceptibility. Participants (N = 41) were sampled, contributing 362 samples for microbiome analysis. All non-Lactobacillus dominant microbial communities were associated with high pro-inflammatory cytokine levels. Divergent associations were observed among different Gardnerella subgroup dominated communities with respect to the chemokine IP-10. Specifically, Gardnerella subgroup A dominant and polymicrobial communities were associated with reduced concentrations of IP-10 in adjusted linear mixed models (p<0.0001), compared to microbial communities dominated by Lactobacillus (non-iners) species. However, these associations did not translate to significant differences in the proportion or absolute number of CCR5, HLA-DR and CD38 expressed on cervical CD4(+) T- cells. These findings suggest that some associations between Gardnerella subgroup dominant microbiomes and mucosal immunity differ and are relevant for the study of BV-pathogenesis and understanding the mechanisms of BV-associated HIV risk.
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spelling pubmed-98864952023-01-31 Gardnerella subgroup dominant microbiomes are associated with divergent cervicovaginal immune responses in a longitudinal cohort of Kenyan women Shvartsman, Elinor Perciani, Catia T. Richmond, Meika E. I. Russell, Justen N. H. Tough, Riley H. Vancuren, Sarah J. Hill, Janet E. KAVI-ICR, Jaoko, Walter McKinnon, Lyle R. Sandstrom, Paul A. MacDonald, Kelly S. Front Immunol Immunology Most cervicovaginal microbiome-immunology studies to date have relied on 16S rDNA microbial profiling which does not resolve the molecular subgroups of Gardnerella, believed to be central to the pathogenesis of bacterial vaginosis (BV) and subsequent risk of HIV acquisition. Here we used the cpn60 universal target which in addition to other microbial taxa, resolves four Gardnerella subgroups, for cervicovaginal microbial profiling in a longitudinal cohort of Kenyan women to examine associations with cellular and soluble markers of inflammation and HIV susceptibility. Participants (N = 41) were sampled, contributing 362 samples for microbiome analysis. All non-Lactobacillus dominant microbial communities were associated with high pro-inflammatory cytokine levels. Divergent associations were observed among different Gardnerella subgroup dominated communities with respect to the chemokine IP-10. Specifically, Gardnerella subgroup A dominant and polymicrobial communities were associated with reduced concentrations of IP-10 in adjusted linear mixed models (p<0.0001), compared to microbial communities dominated by Lactobacillus (non-iners) species. However, these associations did not translate to significant differences in the proportion or absolute number of CCR5, HLA-DR and CD38 expressed on cervical CD4(+) T- cells. These findings suggest that some associations between Gardnerella subgroup dominant microbiomes and mucosal immunity differ and are relevant for the study of BV-pathogenesis and understanding the mechanisms of BV-associated HIV risk. Frontiers Media S.A. 2023-01-16 /pmc/articles/PMC9886495/ /pubmed/36726972 http://dx.doi.org/10.3389/fimmu.2022.974195 Text en Copyright © 2023 Shvartsman, Perciani, Richmond, Russell, Tough, Vancuren, Hill, KAVI-ICR, Jaoko, McKinnon, Sandstrom and MacDonald https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Shvartsman, Elinor
Perciani, Catia T.
Richmond, Meika E. I.
Russell, Justen N. H.
Tough, Riley H.
Vancuren, Sarah J.
Hill, Janet E.
KAVI-ICR,
Jaoko, Walter
McKinnon, Lyle R.
Sandstrom, Paul A.
MacDonald, Kelly S.
Gardnerella subgroup dominant microbiomes are associated with divergent cervicovaginal immune responses in a longitudinal cohort of Kenyan women
title Gardnerella subgroup dominant microbiomes are associated with divergent cervicovaginal immune responses in a longitudinal cohort of Kenyan women
title_full Gardnerella subgroup dominant microbiomes are associated with divergent cervicovaginal immune responses in a longitudinal cohort of Kenyan women
title_fullStr Gardnerella subgroup dominant microbiomes are associated with divergent cervicovaginal immune responses in a longitudinal cohort of Kenyan women
title_full_unstemmed Gardnerella subgroup dominant microbiomes are associated with divergent cervicovaginal immune responses in a longitudinal cohort of Kenyan women
title_short Gardnerella subgroup dominant microbiomes are associated with divergent cervicovaginal immune responses in a longitudinal cohort of Kenyan women
title_sort gardnerella subgroup dominant microbiomes are associated with divergent cervicovaginal immune responses in a longitudinal cohort of kenyan women
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9886495/
https://www.ncbi.nlm.nih.gov/pubmed/36726972
http://dx.doi.org/10.3389/fimmu.2022.974195
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