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Identification and characterization of pathogenicity-related genes of Rhizoctonia solani AG3 during tobacco infection

Tobacco target spot disease is caused by a ubiquitous soil-borne phytopathogen Rhizoctonia solani; the pathogenic mechanisms underlying the effects of R. solani remain unclear. Deeper understanding of the functional responses to R. solani during host plant infection would help identify the molecular...

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Autores principales: Tang, Qianjun, Ma, Qianqian, Xiao, Zhipeng, Xiao, Yansong, Wang, Yunsheng, Liu, Lei, Peng, Weiye, Wang, Bing, Liu, Tianbo, Song, Na
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9887180/
https://www.ncbi.nlm.nih.gov/pubmed/36733585
http://dx.doi.org/10.3389/fpls.2022.1116506
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author Tang, Qianjun
Ma, Qianqian
Xiao, Zhipeng
Xiao, Yansong
Wang, Yunsheng
Liu, Lei
Peng, Weiye
Wang, Bing
Liu, Tianbo
Song, Na
author_facet Tang, Qianjun
Ma, Qianqian
Xiao, Zhipeng
Xiao, Yansong
Wang, Yunsheng
Liu, Lei
Peng, Weiye
Wang, Bing
Liu, Tianbo
Song, Na
author_sort Tang, Qianjun
collection PubMed
description Tobacco target spot disease is caused by a ubiquitous soil-borne phytopathogen Rhizoctonia solani; the pathogenic mechanisms underlying the effects of R. solani remain unclear. Deeper understanding of the functional responses to R. solani during host plant infection would help identify the molecular mechanisms essential for successful host invasion. In this study, we performed global transcriptional analysis of R. solani during various stages (12, 24, 48, 72, 96, and 120 h) of tobacco infection via an RNA sequencing method, while utilizing the pathosystem model R. solani AG3–tobacco (Nicotiana tabacum L.). After R. solani inoculation, the number of differentially expressed genes of R. solani differed at the various time points. Moreover, several gene ontology and Kyoto encyclopedia of genes and genomes pathways were unique in different infection stages, especially with respect to the genes involved in plant cell wall degradation and catalysis of biotransformation reactions, such as the pectin metabolic process and pectin catabolic process. The overexpressing-PD8 N. benthamiana plants enhanced the susceptibility to R. solani. In addition, we found that large amounts of reactive oxygen species (ROS) were generated in tobacco after infected by R. solani. R. solani encoding FAD/NAD binding oxidoreductase and peroxidase gene family to eliminating ROS and counteract oxidative stress. Moreover, Perox3 was validated that can enhance the ability of scavenging ROS by co-injecting. Overall, our findings show that pectin-degrading enzymes and cytochrome P450 genes are critical for plant infection. These results provide comprehensive insights into R. solani AG3 transcriptome responses during tobacco invasion.
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spelling pubmed-98871802023-02-01 Identification and characterization of pathogenicity-related genes of Rhizoctonia solani AG3 during tobacco infection Tang, Qianjun Ma, Qianqian Xiao, Zhipeng Xiao, Yansong Wang, Yunsheng Liu, Lei Peng, Weiye Wang, Bing Liu, Tianbo Song, Na Front Plant Sci Plant Science Tobacco target spot disease is caused by a ubiquitous soil-borne phytopathogen Rhizoctonia solani; the pathogenic mechanisms underlying the effects of R. solani remain unclear. Deeper understanding of the functional responses to R. solani during host plant infection would help identify the molecular mechanisms essential for successful host invasion. In this study, we performed global transcriptional analysis of R. solani during various stages (12, 24, 48, 72, 96, and 120 h) of tobacco infection via an RNA sequencing method, while utilizing the pathosystem model R. solani AG3–tobacco (Nicotiana tabacum L.). After R. solani inoculation, the number of differentially expressed genes of R. solani differed at the various time points. Moreover, several gene ontology and Kyoto encyclopedia of genes and genomes pathways were unique in different infection stages, especially with respect to the genes involved in plant cell wall degradation and catalysis of biotransformation reactions, such as the pectin metabolic process and pectin catabolic process. The overexpressing-PD8 N. benthamiana plants enhanced the susceptibility to R. solani. In addition, we found that large amounts of reactive oxygen species (ROS) were generated in tobacco after infected by R. solani. R. solani encoding FAD/NAD binding oxidoreductase and peroxidase gene family to eliminating ROS and counteract oxidative stress. Moreover, Perox3 was validated that can enhance the ability of scavenging ROS by co-injecting. Overall, our findings show that pectin-degrading enzymes and cytochrome P450 genes are critical for plant infection. These results provide comprehensive insights into R. solani AG3 transcriptome responses during tobacco invasion. Frontiers Media S.A. 2023-01-17 /pmc/articles/PMC9887180/ /pubmed/36733585 http://dx.doi.org/10.3389/fpls.2022.1116506 Text en Copyright © 2023 Tang, Ma, Xiao, Xiao, Wang, Liu, Peng, Wang, Liu and Song https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Tang, Qianjun
Ma, Qianqian
Xiao, Zhipeng
Xiao, Yansong
Wang, Yunsheng
Liu, Lei
Peng, Weiye
Wang, Bing
Liu, Tianbo
Song, Na
Identification and characterization of pathogenicity-related genes of Rhizoctonia solani AG3 during tobacco infection
title Identification and characterization of pathogenicity-related genes of Rhizoctonia solani AG3 during tobacco infection
title_full Identification and characterization of pathogenicity-related genes of Rhizoctonia solani AG3 during tobacco infection
title_fullStr Identification and characterization of pathogenicity-related genes of Rhizoctonia solani AG3 during tobacco infection
title_full_unstemmed Identification and characterization of pathogenicity-related genes of Rhizoctonia solani AG3 during tobacco infection
title_short Identification and characterization of pathogenicity-related genes of Rhizoctonia solani AG3 during tobacco infection
title_sort identification and characterization of pathogenicity-related genes of rhizoctonia solani ag3 during tobacco infection
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9887180/
https://www.ncbi.nlm.nih.gov/pubmed/36733585
http://dx.doi.org/10.3389/fpls.2022.1116506
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