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Bone-marrow derived cells do not contribute to new beta-cells in the inflamed pancreas
The contribution of bone-marrow derived cells (BMCs) to a newly formed beta-cell population in adults is controversial. Previous studies have only used models of bone marrow transplantation from sex-mismatched donors (or other models of genetic labeling) into recipient animals that had undergone irr...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9887320/ https://www.ncbi.nlm.nih.gov/pubmed/36733483 http://dx.doi.org/10.3389/fimmu.2023.1084056 |
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author | Jiang, Yinan Wiersch, John Wu, Wei Qian, Jieqi Adama, Maharana Prathap R. Wu, Nannan Yang, Weixia Chen, Congde Zhu, Lingyan Prasadan, Krishna Gittes, George K. Xiao, Xiangwei |
author_facet | Jiang, Yinan Wiersch, John Wu, Wei Qian, Jieqi Adama, Maharana Prathap R. Wu, Nannan Yang, Weixia Chen, Congde Zhu, Lingyan Prasadan, Krishna Gittes, George K. Xiao, Xiangwei |
author_sort | Jiang, Yinan |
collection | PubMed |
description | The contribution of bone-marrow derived cells (BMCs) to a newly formed beta-cell population in adults is controversial. Previous studies have only used models of bone marrow transplantation from sex-mismatched donors (or other models of genetic labeling) into recipient animals that had undergone irradiation. This approach suffers from the significant shortcoming of the off-target effects of irradiation. Partial pancreatic duct ligation (PDL) is a mouse model of acute pancreatitis with a modest increase in beta-cell number. However, the possibility that recruited BMCs in the inflamed pancreas may convert into beta-cells has not been examined. Here, we used an irradiation-free model to track the fate of the BMCs from the donor mice. A ROSA-mTmG red fluorescent mouse was surgically joined to an INS1(Cre) knock-in mouse by parabiosis to establish a mixed circulation. PDL was then performed in the INS1(Cre) mice 2 weeks after parabiosis, which was one week after establishment of the stable blood chimera. The contribution of red cells from ROSA-mTmG mice to beta-cells in INS1(Cre) mouse was evaluated based on red fluorescence, while cell fusion was evaluated by the presence of green fluorescence in beta-cells. We did not detect any red or green insulin+ cells in the INS1(Cre) mice, suggesting that there was no contribution of BMCs to the newly formed beta-cells, either by direct differentiation, or by cell fusion. Thus, the contribution of BMCs to beta-cells in the inflamed pancreas should be minimal, if any. |
format | Online Article Text |
id | pubmed-9887320 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-98873202023-02-01 Bone-marrow derived cells do not contribute to new beta-cells in the inflamed pancreas Jiang, Yinan Wiersch, John Wu, Wei Qian, Jieqi Adama, Maharana Prathap R. Wu, Nannan Yang, Weixia Chen, Congde Zhu, Lingyan Prasadan, Krishna Gittes, George K. Xiao, Xiangwei Front Immunol Immunology The contribution of bone-marrow derived cells (BMCs) to a newly formed beta-cell population in adults is controversial. Previous studies have only used models of bone marrow transplantation from sex-mismatched donors (or other models of genetic labeling) into recipient animals that had undergone irradiation. This approach suffers from the significant shortcoming of the off-target effects of irradiation. Partial pancreatic duct ligation (PDL) is a mouse model of acute pancreatitis with a modest increase in beta-cell number. However, the possibility that recruited BMCs in the inflamed pancreas may convert into beta-cells has not been examined. Here, we used an irradiation-free model to track the fate of the BMCs from the donor mice. A ROSA-mTmG red fluorescent mouse was surgically joined to an INS1(Cre) knock-in mouse by parabiosis to establish a mixed circulation. PDL was then performed in the INS1(Cre) mice 2 weeks after parabiosis, which was one week after establishment of the stable blood chimera. The contribution of red cells from ROSA-mTmG mice to beta-cells in INS1(Cre) mouse was evaluated based on red fluorescence, while cell fusion was evaluated by the presence of green fluorescence in beta-cells. We did not detect any red or green insulin+ cells in the INS1(Cre) mice, suggesting that there was no contribution of BMCs to the newly formed beta-cells, either by direct differentiation, or by cell fusion. Thus, the contribution of BMCs to beta-cells in the inflamed pancreas should be minimal, if any. Frontiers Media S.A. 2023-01-17 /pmc/articles/PMC9887320/ /pubmed/36733483 http://dx.doi.org/10.3389/fimmu.2023.1084056 Text en Copyright © 2023 Jiang, Wiersch, Wu, Qian, Adama, Wu, Yang, Chen, Zhu, Prasadan, Gittes and Xiao https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology Jiang, Yinan Wiersch, John Wu, Wei Qian, Jieqi Adama, Maharana Prathap R. Wu, Nannan Yang, Weixia Chen, Congde Zhu, Lingyan Prasadan, Krishna Gittes, George K. Xiao, Xiangwei Bone-marrow derived cells do not contribute to new beta-cells in the inflamed pancreas |
title | Bone-marrow derived cells do not contribute to new beta-cells in the inflamed pancreas |
title_full | Bone-marrow derived cells do not contribute to new beta-cells in the inflamed pancreas |
title_fullStr | Bone-marrow derived cells do not contribute to new beta-cells in the inflamed pancreas |
title_full_unstemmed | Bone-marrow derived cells do not contribute to new beta-cells in the inflamed pancreas |
title_short | Bone-marrow derived cells do not contribute to new beta-cells in the inflamed pancreas |
title_sort | bone-marrow derived cells do not contribute to new beta-cells in the inflamed pancreas |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9887320/ https://www.ncbi.nlm.nih.gov/pubmed/36733483 http://dx.doi.org/10.3389/fimmu.2023.1084056 |
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