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Disinfectant Susceptibility of Third-Generation-Cephalosporin/Carbapenem-Resistant Gram-Negative Bacteria Isolated from the Oral Cavity of Residents of Long-Term-Care Facilities

We have recently reported the isolation of third-generation-cephalosporin-resistant Gram-negative bacteria from the oral cavity of residents of a long-term-care facility (LTCF). Since disinfectants are often used in the oral cavity, it is important to investigate the disinfectant susceptibility of o...

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Autores principales: Haruta, Azusa, Kawada-Matsuo, Miki, Le, Mi Nguyen-Tra, Yoshikawa, Mineka, Kajihara, Toshiki, Yahara, Koji, Kitamura, Norikazu, Kutsuno, Shoko, Arai, Chika, Takeuchi, Maho, Sugawara, Yo, Hisatsune, Junzo, Tsuga, Kazuhiro, Ohge, Hiroki, Sugai, Motoyuki, Komatsuzawa, Hitoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9888285/
https://www.ncbi.nlm.nih.gov/pubmed/36515531
http://dx.doi.org/10.1128/aem.01712-22
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author Haruta, Azusa
Kawada-Matsuo, Miki
Le, Mi Nguyen-Tra
Yoshikawa, Mineka
Kajihara, Toshiki
Yahara, Koji
Kitamura, Norikazu
Kutsuno, Shoko
Arai, Chika
Takeuchi, Maho
Sugawara, Yo
Hisatsune, Junzo
Tsuga, Kazuhiro
Ohge, Hiroki
Sugai, Motoyuki
Komatsuzawa, Hitoshi
author_facet Haruta, Azusa
Kawada-Matsuo, Miki
Le, Mi Nguyen-Tra
Yoshikawa, Mineka
Kajihara, Toshiki
Yahara, Koji
Kitamura, Norikazu
Kutsuno, Shoko
Arai, Chika
Takeuchi, Maho
Sugawara, Yo
Hisatsune, Junzo
Tsuga, Kazuhiro
Ohge, Hiroki
Sugai, Motoyuki
Komatsuzawa, Hitoshi
author_sort Haruta, Azusa
collection PubMed
description We have recently reported the isolation of third-generation-cephalosporin-resistant Gram-negative bacteria from the oral cavity of residents of a long-term-care facility (LTCF). Since disinfectants are often used in the oral cavity, it is important to investigate the disinfectant susceptibility of oral bacteria. Here, we evaluated the susceptibilities of Gram-negative antimicrobial-resistant bacteria (GN-ARB), including Pseudomonas, Acinetobacter, and Enterobacteriaceae, obtained from the oral cavity of residents of LTCFs to povidone-iodine (PVPI), cetylpyridinium chloride (CPC), benzalkonium chloride (BZK), and chlorhexidine chloride (CHX). We also evaluated the susceptibilities of isolates from the rectum to the same agents to compare the susceptibility profiles of oral and rectal isolates. Next, we investigated the relationship between their susceptibility and disinfectant resistance genes delineated by whole-genome sequencing of the isolates. Additionally, we evaluated the correlation between disinfectant-resistant GN-ARB and clinical information. In oral GN-ARB, the MIC of PVPI showed almost identical values across isolates, while the MICs of CPC, BZK, and CHX showed a wide range of variation among species/strains. In particular, Pseudomonas aeruginosa exhibited high-level resistance to CPC and BZK. The disinfectant susceptibility of rectal GN-ARB showed a tendency similar to that of oral GN-ARB. The presence of qacEΔ1 was correlated with CPC/BZK resistance in P. aeruginosa, while other species exhibited no correlation between qacEΔ1 and resistance. Multiple analyses showed the correlation between the presence of CPC-resistant bacteria in the oral cavity and tube feeding. In conclusion, we found that some oral GN-ARB isolates showed resistance to not only antibiotics but also disinfectants. IMPORTANCE Antibiotic-resistant bacteria (ARB) are becoming a serious concern worldwide. We previously reported the isolation of third-generation-cephalosporin-resistant Gram-negative bacteria from the oral cavity of residents of a long-term-care facility (LTCF). To prevent infection with ARB in hospitals and eldercare facilities, we must pay more attention to the use of not only antibiotics but also disinfectants. However, the effect of disinfectants on ARB is unclear. In this study, we evaluated the susceptibility of Gram-negative ARB (GN-ARB) from the oral cavity of residents of LTCFs to some disinfectants that are often used for the oral cavity; we found that some isolates showed resistance to several disinfectants. This is the first comprehensive analysis of the disinfectant susceptibility of oral GN-ARB. These results provide some important information for infection control and suggest that disinfectants should be applied carefully.
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spelling pubmed-98882852023-02-01 Disinfectant Susceptibility of Third-Generation-Cephalosporin/Carbapenem-Resistant Gram-Negative Bacteria Isolated from the Oral Cavity of Residents of Long-Term-Care Facilities Haruta, Azusa Kawada-Matsuo, Miki Le, Mi Nguyen-Tra Yoshikawa, Mineka Kajihara, Toshiki Yahara, Koji Kitamura, Norikazu Kutsuno, Shoko Arai, Chika Takeuchi, Maho Sugawara, Yo Hisatsune, Junzo Tsuga, Kazuhiro Ohge, Hiroki Sugai, Motoyuki Komatsuzawa, Hitoshi Appl Environ Microbiol Environmental Microbiology We have recently reported the isolation of third-generation-cephalosporin-resistant Gram-negative bacteria from the oral cavity of residents of a long-term-care facility (LTCF). Since disinfectants are often used in the oral cavity, it is important to investigate the disinfectant susceptibility of oral bacteria. Here, we evaluated the susceptibilities of Gram-negative antimicrobial-resistant bacteria (GN-ARB), including Pseudomonas, Acinetobacter, and Enterobacteriaceae, obtained from the oral cavity of residents of LTCFs to povidone-iodine (PVPI), cetylpyridinium chloride (CPC), benzalkonium chloride (BZK), and chlorhexidine chloride (CHX). We also evaluated the susceptibilities of isolates from the rectum to the same agents to compare the susceptibility profiles of oral and rectal isolates. Next, we investigated the relationship between their susceptibility and disinfectant resistance genes delineated by whole-genome sequencing of the isolates. Additionally, we evaluated the correlation between disinfectant-resistant GN-ARB and clinical information. In oral GN-ARB, the MIC of PVPI showed almost identical values across isolates, while the MICs of CPC, BZK, and CHX showed a wide range of variation among species/strains. In particular, Pseudomonas aeruginosa exhibited high-level resistance to CPC and BZK. The disinfectant susceptibility of rectal GN-ARB showed a tendency similar to that of oral GN-ARB. The presence of qacEΔ1 was correlated with CPC/BZK resistance in P. aeruginosa, while other species exhibited no correlation between qacEΔ1 and resistance. Multiple analyses showed the correlation between the presence of CPC-resistant bacteria in the oral cavity and tube feeding. In conclusion, we found that some oral GN-ARB isolates showed resistance to not only antibiotics but also disinfectants. IMPORTANCE Antibiotic-resistant bacteria (ARB) are becoming a serious concern worldwide. We previously reported the isolation of third-generation-cephalosporin-resistant Gram-negative bacteria from the oral cavity of residents of a long-term-care facility (LTCF). To prevent infection with ARB in hospitals and eldercare facilities, we must pay more attention to the use of not only antibiotics but also disinfectants. However, the effect of disinfectants on ARB is unclear. In this study, we evaluated the susceptibility of Gram-negative ARB (GN-ARB) from the oral cavity of residents of LTCFs to some disinfectants that are often used for the oral cavity; we found that some isolates showed resistance to several disinfectants. This is the first comprehensive analysis of the disinfectant susceptibility of oral GN-ARB. These results provide some important information for infection control and suggest that disinfectants should be applied carefully. American Society for Microbiology 2022-12-14 /pmc/articles/PMC9888285/ /pubmed/36515531 http://dx.doi.org/10.1128/aem.01712-22 Text en Copyright © 2022 Haruta et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Environmental Microbiology
Haruta, Azusa
Kawada-Matsuo, Miki
Le, Mi Nguyen-Tra
Yoshikawa, Mineka
Kajihara, Toshiki
Yahara, Koji
Kitamura, Norikazu
Kutsuno, Shoko
Arai, Chika
Takeuchi, Maho
Sugawara, Yo
Hisatsune, Junzo
Tsuga, Kazuhiro
Ohge, Hiroki
Sugai, Motoyuki
Komatsuzawa, Hitoshi
Disinfectant Susceptibility of Third-Generation-Cephalosporin/Carbapenem-Resistant Gram-Negative Bacteria Isolated from the Oral Cavity of Residents of Long-Term-Care Facilities
title Disinfectant Susceptibility of Third-Generation-Cephalosporin/Carbapenem-Resistant Gram-Negative Bacteria Isolated from the Oral Cavity of Residents of Long-Term-Care Facilities
title_full Disinfectant Susceptibility of Third-Generation-Cephalosporin/Carbapenem-Resistant Gram-Negative Bacteria Isolated from the Oral Cavity of Residents of Long-Term-Care Facilities
title_fullStr Disinfectant Susceptibility of Third-Generation-Cephalosporin/Carbapenem-Resistant Gram-Negative Bacteria Isolated from the Oral Cavity of Residents of Long-Term-Care Facilities
title_full_unstemmed Disinfectant Susceptibility of Third-Generation-Cephalosporin/Carbapenem-Resistant Gram-Negative Bacteria Isolated from the Oral Cavity of Residents of Long-Term-Care Facilities
title_short Disinfectant Susceptibility of Third-Generation-Cephalosporin/Carbapenem-Resistant Gram-Negative Bacteria Isolated from the Oral Cavity of Residents of Long-Term-Care Facilities
title_sort disinfectant susceptibility of third-generation-cephalosporin/carbapenem-resistant gram-negative bacteria isolated from the oral cavity of residents of long-term-care facilities
topic Environmental Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9888285/
https://www.ncbi.nlm.nih.gov/pubmed/36515531
http://dx.doi.org/10.1128/aem.01712-22
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