Cargando…
Effects of Dietary Nonfibrous Carbohydrate/Neutral Detergent Fiber Ratio on Methanogenic Archaea and Cellulose-Degrading Bacteria in the Rumen of Karakul Sheep: a 16S rRNA Gene Sequencing Study
The study was conducted to investigate the effects of dietary nonfibrous carbohydrate (NFC)/neutral detergent fiber (NDF) ratio on methanogenic archaea and cellulose-degrading bacteria in Karakul sheep by 16S rRNA gene sequencing. Twelve Karakul sheep were randomly divided into four groups, each gro...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Microbiology
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9888294/ https://www.ncbi.nlm.nih.gov/pubmed/36541769 http://dx.doi.org/10.1128/aem.01291-22 |
_version_ | 1784880503192551424 |
---|---|
author | Bai, Tiantian Pu, Xuanxuan Guo, Xuefeng Liu, Junfeng Zhao, Linbo Zhang, Xiuping Zhang, Sujiang Cheng, Long |
author_facet | Bai, Tiantian Pu, Xuanxuan Guo, Xuefeng Liu, Junfeng Zhao, Linbo Zhang, Xiuping Zhang, Sujiang Cheng, Long |
author_sort | Bai, Tiantian |
collection | PubMed |
description | The study was conducted to investigate the effects of dietary nonfibrous carbohydrate (NFC)/neutral detergent fiber (NDF) ratio on methanogenic archaea and cellulose-degrading bacteria in Karakul sheep by 16S rRNA gene sequencing. Twelve Karakul sheep were randomly divided into four groups, each group with three replicates, and they were fed with four dietary NFC/NDF ratios at 0.54, 0.96, 1.37, and 1.90 as groups 1, 2, 3, and 4, respectively. The experiment lasted for four periods: I (1 to 18 days), II (19 to 36 days), III (37 to 54 days), and IV (55 to 72 days); during each period, rumen contents were collected before morning feeding to investigate on methanogenic archaea and cellulose-degrading bacteria. The results showed that with an increase in dietary NFC/NDF ratio, the number of rumen archaea operational taxonomic units and the diversity of archaea decrease. The most dominant methanogens did not change with dietary NFC/NDF ratio and prolongation of experimental periods. Methanobrevibacter was the most dominant genus. At the species level, the relative abundance of Methanobrevibacter ruminantium first increased and then decreased when the NFC/NDF ratio increased. When the dietary NFC/NDF ratio was 0.96, the structure of archaea was largely changed, and the relative abundance of Fibrobacter sp. strain UWCM, Ruminococcus flavefaciens, and Ruminococcus albus were the highest. When the dietary NFC/NDF ratio was 1.37, the relative abundance of Butyrivibrio fibrisolvens was higher than for other groups. Based on all the data, we concluded that a dietary NFC/NDF ratio of ca. 0.96 to 1.37 was a suitable ratio to support optimal sheep production. IMPORTANCE CH(4) produced by ruminants aggravates the greenhouse effect and cause wastage of feed energy, and CH(4) emissions are related to methanogens. According to the current literature, there is a symbiotic relationship between methanogens and cellulolytic bacteria, so reducing methane will inevitably affect the degradation of fiber materials. This experiment used 16S rRNA gene high-throughput sequencing technology to explore the balance relationship between methanogens and cellulolytic bacteria for the first time through a long-term feeding period. The findings provide fundamental data, supporting for the diet structures with potential to reduce CH(4) emission. |
format | Online Article Text |
id | pubmed-9888294 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | American Society for Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-98882942023-02-01 Effects of Dietary Nonfibrous Carbohydrate/Neutral Detergent Fiber Ratio on Methanogenic Archaea and Cellulose-Degrading Bacteria in the Rumen of Karakul Sheep: a 16S rRNA Gene Sequencing Study Bai, Tiantian Pu, Xuanxuan Guo, Xuefeng Liu, Junfeng Zhao, Linbo Zhang, Xiuping Zhang, Sujiang Cheng, Long Appl Environ Microbiol Evolutionary and Genomic Microbiology The study was conducted to investigate the effects of dietary nonfibrous carbohydrate (NFC)/neutral detergent fiber (NDF) ratio on methanogenic archaea and cellulose-degrading bacteria in Karakul sheep by 16S rRNA gene sequencing. Twelve Karakul sheep were randomly divided into four groups, each group with three replicates, and they were fed with four dietary NFC/NDF ratios at 0.54, 0.96, 1.37, and 1.90 as groups 1, 2, 3, and 4, respectively. The experiment lasted for four periods: I (1 to 18 days), II (19 to 36 days), III (37 to 54 days), and IV (55 to 72 days); during each period, rumen contents were collected before morning feeding to investigate on methanogenic archaea and cellulose-degrading bacteria. The results showed that with an increase in dietary NFC/NDF ratio, the number of rumen archaea operational taxonomic units and the diversity of archaea decrease. The most dominant methanogens did not change with dietary NFC/NDF ratio and prolongation of experimental periods. Methanobrevibacter was the most dominant genus. At the species level, the relative abundance of Methanobrevibacter ruminantium first increased and then decreased when the NFC/NDF ratio increased. When the dietary NFC/NDF ratio was 0.96, the structure of archaea was largely changed, and the relative abundance of Fibrobacter sp. strain UWCM, Ruminococcus flavefaciens, and Ruminococcus albus were the highest. When the dietary NFC/NDF ratio was 1.37, the relative abundance of Butyrivibrio fibrisolvens was higher than for other groups. Based on all the data, we concluded that a dietary NFC/NDF ratio of ca. 0.96 to 1.37 was a suitable ratio to support optimal sheep production. IMPORTANCE CH(4) produced by ruminants aggravates the greenhouse effect and cause wastage of feed energy, and CH(4) emissions are related to methanogens. According to the current literature, there is a symbiotic relationship between methanogens and cellulolytic bacteria, so reducing methane will inevitably affect the degradation of fiber materials. This experiment used 16S rRNA gene high-throughput sequencing technology to explore the balance relationship between methanogens and cellulolytic bacteria for the first time through a long-term feeding period. The findings provide fundamental data, supporting for the diet structures with potential to reduce CH(4) emission. American Society for Microbiology 2022-12-21 /pmc/articles/PMC9888294/ /pubmed/36541769 http://dx.doi.org/10.1128/aem.01291-22 Text en Copyright © 2022 Bai et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Evolutionary and Genomic Microbiology Bai, Tiantian Pu, Xuanxuan Guo, Xuefeng Liu, Junfeng Zhao, Linbo Zhang, Xiuping Zhang, Sujiang Cheng, Long Effects of Dietary Nonfibrous Carbohydrate/Neutral Detergent Fiber Ratio on Methanogenic Archaea and Cellulose-Degrading Bacteria in the Rumen of Karakul Sheep: a 16S rRNA Gene Sequencing Study |
title | Effects of Dietary Nonfibrous Carbohydrate/Neutral Detergent Fiber Ratio on Methanogenic Archaea and Cellulose-Degrading Bacteria in the Rumen of Karakul Sheep: a 16S rRNA Gene Sequencing Study |
title_full | Effects of Dietary Nonfibrous Carbohydrate/Neutral Detergent Fiber Ratio on Methanogenic Archaea and Cellulose-Degrading Bacteria in the Rumen of Karakul Sheep: a 16S rRNA Gene Sequencing Study |
title_fullStr | Effects of Dietary Nonfibrous Carbohydrate/Neutral Detergent Fiber Ratio on Methanogenic Archaea and Cellulose-Degrading Bacteria in the Rumen of Karakul Sheep: a 16S rRNA Gene Sequencing Study |
title_full_unstemmed | Effects of Dietary Nonfibrous Carbohydrate/Neutral Detergent Fiber Ratio on Methanogenic Archaea and Cellulose-Degrading Bacteria in the Rumen of Karakul Sheep: a 16S rRNA Gene Sequencing Study |
title_short | Effects of Dietary Nonfibrous Carbohydrate/Neutral Detergent Fiber Ratio on Methanogenic Archaea and Cellulose-Degrading Bacteria in the Rumen of Karakul Sheep: a 16S rRNA Gene Sequencing Study |
title_sort | effects of dietary nonfibrous carbohydrate/neutral detergent fiber ratio on methanogenic archaea and cellulose-degrading bacteria in the rumen of karakul sheep: a 16s rrna gene sequencing study |
topic | Evolutionary and Genomic Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9888294/ https://www.ncbi.nlm.nih.gov/pubmed/36541769 http://dx.doi.org/10.1128/aem.01291-22 |
work_keys_str_mv | AT baitiantian effectsofdietarynonfibrouscarbohydrateneutraldetergentfiberratioonmethanogenicarchaeaandcellulosedegradingbacteriaintherumenofkarakulsheepa16srrnagenesequencingstudy AT puxuanxuan effectsofdietarynonfibrouscarbohydrateneutraldetergentfiberratioonmethanogenicarchaeaandcellulosedegradingbacteriaintherumenofkarakulsheepa16srrnagenesequencingstudy AT guoxuefeng effectsofdietarynonfibrouscarbohydrateneutraldetergentfiberratioonmethanogenicarchaeaandcellulosedegradingbacteriaintherumenofkarakulsheepa16srrnagenesequencingstudy AT liujunfeng effectsofdietarynonfibrouscarbohydrateneutraldetergentfiberratioonmethanogenicarchaeaandcellulosedegradingbacteriaintherumenofkarakulsheepa16srrnagenesequencingstudy AT zhaolinbo effectsofdietarynonfibrouscarbohydrateneutraldetergentfiberratioonmethanogenicarchaeaandcellulosedegradingbacteriaintherumenofkarakulsheepa16srrnagenesequencingstudy AT zhangxiuping effectsofdietarynonfibrouscarbohydrateneutraldetergentfiberratioonmethanogenicarchaeaandcellulosedegradingbacteriaintherumenofkarakulsheepa16srrnagenesequencingstudy AT zhangsujiang effectsofdietarynonfibrouscarbohydrateneutraldetergentfiberratioonmethanogenicarchaeaandcellulosedegradingbacteriaintherumenofkarakulsheepa16srrnagenesequencingstudy AT chenglong effectsofdietarynonfibrouscarbohydrateneutraldetergentfiberratioonmethanogenicarchaeaandcellulosedegradingbacteriaintherumenofkarakulsheepa16srrnagenesequencingstudy |