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Synthesis of Gingerol-Metals Complex and in-vitro Cytotoxic Activity on Human Colon Cancer Cell Line

INTRODUCTION: Herbs are excellent sources of medicinal substances, and their curative abilities have been recognized to treat many ailments and are used for example as antioxidants, analgesics, anti-inflammatories, antipyretics, and many other medicinal uses. The properties of natural compounds and...

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Autores principales: Khdary, Nezar H, Alangari, Abdulaziz A, Katubi, Khadijah M, Alanazi, Mohammad, Alhassan, Ahmed, Alzahrani, Sami D, Khan, Zahid, Alanazi, Ibrahim O
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9888304/
https://www.ncbi.nlm.nih.gov/pubmed/36733670
http://dx.doi.org/10.2147/CMAR.S391546
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author Khdary, Nezar H
Alangari, Abdulaziz A
Katubi, Khadijah M
Alanazi, Mohammad
Alhassan, Ahmed
Alzahrani, Sami D
Khan, Zahid
Alanazi, Ibrahim O
author_facet Khdary, Nezar H
Alangari, Abdulaziz A
Katubi, Khadijah M
Alanazi, Mohammad
Alhassan, Ahmed
Alzahrani, Sami D
Khan, Zahid
Alanazi, Ibrahim O
author_sort Khdary, Nezar H
collection PubMed
description INTRODUCTION: Herbs are excellent sources of medicinal substances, and their curative abilities have been recognized to treat many ailments and are used for example as antioxidants, analgesics, anti-inflammatories, antipyretics, and many other medicinal uses. The properties of natural compounds and their health effects have been studied extensively, especially those that originate from plant sources such as ginger. The ginger plant contains many chemical compounds, such as 6-gingerol, which is characterized by containing active groups such as carbonyl and hydroxide, which can be attached to metal molecules. This is what was done in this study, where the formation of complexes with a group of metals was studied and their effect on cancer cells was investigated. These complexes will open new horizons for further study of medicinal uses. METHODS: The synthesis of gingerol-metal complexes was carried out by conjugating gingerol molecules with Ag, Au, Cd, Co, Cu, Ni, and Zn metal ions. The extracted gingerol was transferred to culture tubes and deionized water-DMSO were added followed by sonication. The tubes were incubated at 90°C for two days as well as the control sample. The samples were then filtered and the complex solutions were transferred into new tubes for further studies. Different characterization techniques such as FT-IR, UV-vis spectroscopy, FESEM, and EDX are used to confirm the formation of the complexes. The in vitro of the complexes was tested by the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay against the human colorectal cancer cell lines HCT116 and HT29 which exhibited strong cytotoxicity. RESULTS: The gingerol-metal complexes showed an enhancement as an anticancer agent compared to the control. The in vitro anticancer activity showed that the Ag-gingerol complex showed the most activity among the other complexes. DISCUSSION: Gingerol-metal complexes can inhibit cancer cells, noting that the potency of the complex depends on the type of metal used.
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spelling pubmed-98883042023-02-01 Synthesis of Gingerol-Metals Complex and in-vitro Cytotoxic Activity on Human Colon Cancer Cell Line Khdary, Nezar H Alangari, Abdulaziz A Katubi, Khadijah M Alanazi, Mohammad Alhassan, Ahmed Alzahrani, Sami D Khan, Zahid Alanazi, Ibrahim O Cancer Manag Res Original Research INTRODUCTION: Herbs are excellent sources of medicinal substances, and their curative abilities have been recognized to treat many ailments and are used for example as antioxidants, analgesics, anti-inflammatories, antipyretics, and many other medicinal uses. The properties of natural compounds and their health effects have been studied extensively, especially those that originate from plant sources such as ginger. The ginger plant contains many chemical compounds, such as 6-gingerol, which is characterized by containing active groups such as carbonyl and hydroxide, which can be attached to metal molecules. This is what was done in this study, where the formation of complexes with a group of metals was studied and their effect on cancer cells was investigated. These complexes will open new horizons for further study of medicinal uses. METHODS: The synthesis of gingerol-metal complexes was carried out by conjugating gingerol molecules with Ag, Au, Cd, Co, Cu, Ni, and Zn metal ions. The extracted gingerol was transferred to culture tubes and deionized water-DMSO were added followed by sonication. The tubes were incubated at 90°C for two days as well as the control sample. The samples were then filtered and the complex solutions were transferred into new tubes for further studies. Different characterization techniques such as FT-IR, UV-vis spectroscopy, FESEM, and EDX are used to confirm the formation of the complexes. The in vitro of the complexes was tested by the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay against the human colorectal cancer cell lines HCT116 and HT29 which exhibited strong cytotoxicity. RESULTS: The gingerol-metal complexes showed an enhancement as an anticancer agent compared to the control. The in vitro anticancer activity showed that the Ag-gingerol complex showed the most activity among the other complexes. DISCUSSION: Gingerol-metal complexes can inhibit cancer cells, noting that the potency of the complex depends on the type of metal used. Dove 2023-01-27 /pmc/articles/PMC9888304/ /pubmed/36733670 http://dx.doi.org/10.2147/CMAR.S391546 Text en © 2023 Khdary et al. https://creativecommons.org/licenses/by-nc/3.0/This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/ (https://creativecommons.org/licenses/by-nc/3.0/) ). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Khdary, Nezar H
Alangari, Abdulaziz A
Katubi, Khadijah M
Alanazi, Mohammad
Alhassan, Ahmed
Alzahrani, Sami D
Khan, Zahid
Alanazi, Ibrahim O
Synthesis of Gingerol-Metals Complex and in-vitro Cytotoxic Activity on Human Colon Cancer Cell Line
title Synthesis of Gingerol-Metals Complex and in-vitro Cytotoxic Activity on Human Colon Cancer Cell Line
title_full Synthesis of Gingerol-Metals Complex and in-vitro Cytotoxic Activity on Human Colon Cancer Cell Line
title_fullStr Synthesis of Gingerol-Metals Complex and in-vitro Cytotoxic Activity on Human Colon Cancer Cell Line
title_full_unstemmed Synthesis of Gingerol-Metals Complex and in-vitro Cytotoxic Activity on Human Colon Cancer Cell Line
title_short Synthesis of Gingerol-Metals Complex and in-vitro Cytotoxic Activity on Human Colon Cancer Cell Line
title_sort synthesis of gingerol-metals complex and in-vitro cytotoxic activity on human colon cancer cell line
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9888304/
https://www.ncbi.nlm.nih.gov/pubmed/36733670
http://dx.doi.org/10.2147/CMAR.S391546
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