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Coronavirus infection in chemosensory cells
Clinical manifestations of human coronavirus (HCoV)-related diseases are mostly related to the respiratory system, although secondary complications such as headache, anosmia, ageusia, and myalgia have been reported. HCoV infection and replication in chemosensory cells associated with ageusia and ano...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer International Publishing
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9888344/ https://www.ncbi.nlm.nih.gov/pubmed/36719595 http://dx.doi.org/10.1007/s13365-023-01117-3 |
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author | Donadoni, Martina Kaminski, Rafal Liao, Shuren Al Janabi, Suhair Margolskee, Robert F. Ozdener, Mehmet Hakan Sariyer, Ilker K. |
author_facet | Donadoni, Martina Kaminski, Rafal Liao, Shuren Al Janabi, Suhair Margolskee, Robert F. Ozdener, Mehmet Hakan Sariyer, Ilker K. |
author_sort | Donadoni, Martina |
collection | PubMed |
description | Clinical manifestations of human coronavirus (HCoV)-related diseases are mostly related to the respiratory system, although secondary complications such as headache, anosmia, ageusia, and myalgia have been reported. HCoV infection and replication in chemosensory cells associated with ageusia and anosmia is poorly understood. Here, we characterized HCoV-OC43 and SARS-CoV-2 infection in two types of chemosensory cells, olfactory and taste cells, with their unique molecular and histological characteristics. We first assessed HCoV-OC43 infection in in vitro cultured human olfactory epithelial cells (hOECs) and fungiform taste papilla (HBO) cells. Interestingly, while both cell types were susceptible to HCoV-OC43 infection, viral replication rates were significantly reduced in HBO cells compared to hOECs. More interestingly, while culture media from hOECs was able to produce secondary infection in Vero cells, there was very limited secondary infection from HBO cells, suggesting that HBO cells may not be able to release infectious virus. On the other hand, unlike HCoV-OC43, SARS-CoV-2 showed comparable levels of viral infection rates in both hOECs and HBO cells. Furthermore, our RT-qPCR-based gene array studies revealed that several key genes involved in taste and olfactory functions were significantly altered by SARS-CoV-2 infection. These results may suggest a possible mechanism associated with chemosensory symptoms, such as anosmia and ageusia in patients infected with SARS-CoV-2. |
format | Online Article Text |
id | pubmed-9888344 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Springer International Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-98883442023-02-01 Coronavirus infection in chemosensory cells Donadoni, Martina Kaminski, Rafal Liao, Shuren Al Janabi, Suhair Margolskee, Robert F. Ozdener, Mehmet Hakan Sariyer, Ilker K. J Neurovirol Article Clinical manifestations of human coronavirus (HCoV)-related diseases are mostly related to the respiratory system, although secondary complications such as headache, anosmia, ageusia, and myalgia have been reported. HCoV infection and replication in chemosensory cells associated with ageusia and anosmia is poorly understood. Here, we characterized HCoV-OC43 and SARS-CoV-2 infection in two types of chemosensory cells, olfactory and taste cells, with their unique molecular and histological characteristics. We first assessed HCoV-OC43 infection in in vitro cultured human olfactory epithelial cells (hOECs) and fungiform taste papilla (HBO) cells. Interestingly, while both cell types were susceptible to HCoV-OC43 infection, viral replication rates were significantly reduced in HBO cells compared to hOECs. More interestingly, while culture media from hOECs was able to produce secondary infection in Vero cells, there was very limited secondary infection from HBO cells, suggesting that HBO cells may not be able to release infectious virus. On the other hand, unlike HCoV-OC43, SARS-CoV-2 showed comparable levels of viral infection rates in both hOECs and HBO cells. Furthermore, our RT-qPCR-based gene array studies revealed that several key genes involved in taste and olfactory functions were significantly altered by SARS-CoV-2 infection. These results may suggest a possible mechanism associated with chemosensory symptoms, such as anosmia and ageusia in patients infected with SARS-CoV-2. Springer International Publishing 2023-01-31 2023 /pmc/articles/PMC9888344/ /pubmed/36719595 http://dx.doi.org/10.1007/s13365-023-01117-3 Text en © The Author(s) under exclusive licence to The Journal of NeuroVirology, Inc. 2023, Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Article Donadoni, Martina Kaminski, Rafal Liao, Shuren Al Janabi, Suhair Margolskee, Robert F. Ozdener, Mehmet Hakan Sariyer, Ilker K. Coronavirus infection in chemosensory cells |
title | Coronavirus infection in chemosensory cells |
title_full | Coronavirus infection in chemosensory cells |
title_fullStr | Coronavirus infection in chemosensory cells |
title_full_unstemmed | Coronavirus infection in chemosensory cells |
title_short | Coronavirus infection in chemosensory cells |
title_sort | coronavirus infection in chemosensory cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9888344/ https://www.ncbi.nlm.nih.gov/pubmed/36719595 http://dx.doi.org/10.1007/s13365-023-01117-3 |
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