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Plasmon–Exciton Coupling Effect in Nanostructured Arrays for Optical Signal Amplification and SARS-CoV-2 DNA Sensing
[Image: see text] A surface plasmon resonance (SPR)-enhanced optical signal using a nanoslit array and acridine orange (AO) dye system at a flexible poly(dimethylsiloxane) (PDMS) substrate was achieved in this work and demonstrated a simple sensing scheme to directly detect SARS-CoV-2 nucleic acid v...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9888407/ https://www.ncbi.nlm.nih.gov/pubmed/36789152 http://dx.doi.org/10.1021/acsanm.2c05063 |
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author | Tukur, Frank Bagra, Bhawna Jayapalan, Anitha Liu, Mengxin Tukur, Panesun Wei, Jianjun |
author_facet | Tukur, Frank Bagra, Bhawna Jayapalan, Anitha Liu, Mengxin Tukur, Panesun Wei, Jianjun |
author_sort | Tukur, Frank |
collection | PubMed |
description | [Image: see text] A surface plasmon resonance (SPR)-enhanced optical signal using a nanoslit array and acridine orange (AO) dye system at a flexible poly(dimethylsiloxane) (PDMS) substrate was achieved in this work and demonstrated a simple sensing scheme to directly detect SARS-CoV-2 nucleic acid via DNA hybridization. A simple nanoimprinting pattern transfer technique was introduced to form uniform reproducible nanoslit arrays where the dimensions of the slit array were controlled by the thickness of the gold film. The plasmon–exciton coupling effect on the optical enhancement of different dye molecules, i.e., AO, propidium iodide (PI), or dihydroethidium (DHE) attached to the nanoslit surfaces, was examined thoroughly by measuring the surface reflection and fluorescence imaging. The results indicate that the best overlap of the plasmon resonance wavelength to the excitation spectrum of AO presented the largest optical enhancement (∼57×) compared to the signal at flat gold surfaces. Based on this finding, a sensitive assay for detecting DNA hybridization was generated using the interaction of the selected SARS-CoV-2 ssDNA and dsDNA with AO to trigger the metachromatic behavior of the dye at the nanoarray surfaces. We found strong optical signal amplification on the formation of acridine-ssDNA complexes and a quenched signal upon hybridization to the complementary target DNA (ct-DNA) along with a blue shift in the fluorescence of AO-dsDNAs. A quantitative evaluation of the ct-DNA concentration in a range of 100–0.08 nM using both the reflection and emission imaging signals demonstrated two linear regimes with a lowest detection limit of 0.21 nM. The sensing method showed high sensitivity and distinguished signals from 1-, 2-, and 3-base mismatched DNA targets, as well as high stability and reusability. This approach toward enhancing optical signal for DNA sensing offers promise in a general, rapid, and direct vision detection method for nucleic acid analytes. |
format | Online Article Text |
id | pubmed-9888407 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-98884072023-02-01 Plasmon–Exciton Coupling Effect in Nanostructured Arrays for Optical Signal Amplification and SARS-CoV-2 DNA Sensing Tukur, Frank Bagra, Bhawna Jayapalan, Anitha Liu, Mengxin Tukur, Panesun Wei, Jianjun ACS Appl Nano Mater [Image: see text] A surface plasmon resonance (SPR)-enhanced optical signal using a nanoslit array and acridine orange (AO) dye system at a flexible poly(dimethylsiloxane) (PDMS) substrate was achieved in this work and demonstrated a simple sensing scheme to directly detect SARS-CoV-2 nucleic acid via DNA hybridization. A simple nanoimprinting pattern transfer technique was introduced to form uniform reproducible nanoslit arrays where the dimensions of the slit array were controlled by the thickness of the gold film. The plasmon–exciton coupling effect on the optical enhancement of different dye molecules, i.e., AO, propidium iodide (PI), or dihydroethidium (DHE) attached to the nanoslit surfaces, was examined thoroughly by measuring the surface reflection and fluorescence imaging. The results indicate that the best overlap of the plasmon resonance wavelength to the excitation spectrum of AO presented the largest optical enhancement (∼57×) compared to the signal at flat gold surfaces. Based on this finding, a sensitive assay for detecting DNA hybridization was generated using the interaction of the selected SARS-CoV-2 ssDNA and dsDNA with AO to trigger the metachromatic behavior of the dye at the nanoarray surfaces. We found strong optical signal amplification on the formation of acridine-ssDNA complexes and a quenched signal upon hybridization to the complementary target DNA (ct-DNA) along with a blue shift in the fluorescence of AO-dsDNAs. A quantitative evaluation of the ct-DNA concentration in a range of 100–0.08 nM using both the reflection and emission imaging signals demonstrated two linear regimes with a lowest detection limit of 0.21 nM. The sensing method showed high sensitivity and distinguished signals from 1-, 2-, and 3-base mismatched DNA targets, as well as high stability and reusability. This approach toward enhancing optical signal for DNA sensing offers promise in a general, rapid, and direct vision detection method for nucleic acid analytes. American Chemical Society 2023-01-24 /pmc/articles/PMC9888407/ /pubmed/36789152 http://dx.doi.org/10.1021/acsanm.2c05063 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Tukur, Frank Bagra, Bhawna Jayapalan, Anitha Liu, Mengxin Tukur, Panesun Wei, Jianjun Plasmon–Exciton Coupling Effect in Nanostructured Arrays for Optical Signal Amplification and SARS-CoV-2 DNA Sensing |
title | Plasmon–Exciton
Coupling Effect in Nanostructured
Arrays for Optical Signal Amplification and SARS-CoV-2 DNA
Sensing |
title_full | Plasmon–Exciton
Coupling Effect in Nanostructured
Arrays for Optical Signal Amplification and SARS-CoV-2 DNA
Sensing |
title_fullStr | Plasmon–Exciton
Coupling Effect in Nanostructured
Arrays for Optical Signal Amplification and SARS-CoV-2 DNA
Sensing |
title_full_unstemmed | Plasmon–Exciton
Coupling Effect in Nanostructured
Arrays for Optical Signal Amplification and SARS-CoV-2 DNA
Sensing |
title_short | Plasmon–Exciton
Coupling Effect in Nanostructured
Arrays for Optical Signal Amplification and SARS-CoV-2 DNA
Sensing |
title_sort | plasmon–exciton
coupling effect in nanostructured
arrays for optical signal amplification and sars-cov-2 dna
sensing |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9888407/ https://www.ncbi.nlm.nih.gov/pubmed/36789152 http://dx.doi.org/10.1021/acsanm.2c05063 |
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