Rapid clonal identification of biallelic CRISPR/Cas9 knock-ins using SNEAK PEEC

One of the challenges faced by current CRISPR/Cas9 editing strategies is the difficulty in rapidly selecting clonal populations of biallelically edited cells. Here we present Surface engiNeered fluorEscence Assisted Kit with Protein Epitope Enhanced Capture (SNEAK PEEC), a platform that combines hum...

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Detalles Bibliográficos
Autores principales: Singh, Sameer, Banerjee, Anoosha, Vanden Broeck, Arnaud, Klinge, Sebastian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9889345/
https://www.ncbi.nlm.nih.gov/pubmed/36720908
http://dx.doi.org/10.1038/s41598-023-28732-8
Descripción
Sumario:One of the challenges faced by current CRISPR/Cas9 editing strategies is the difficulty in rapidly selecting clonal populations of biallelically edited cells. Here we present Surface engiNeered fluorEscence Assisted Kit with Protein Epitope Enhanced Capture (SNEAK PEEC), a platform that combines human genome editing with cell-surface display, which enables the direct identification of biallelically edited clones with minimal screening.

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