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Rapid clonal identification of biallelic CRISPR/Cas9 knock-ins using SNEAK PEEC
One of the challenges faced by current CRISPR/Cas9 editing strategies is the difficulty in rapidly selecting clonal populations of biallelically edited cells. Here we present Surface engiNeered fluorEscence Assisted Kit with Protein Epitope Enhanced Capture (SNEAK PEEC), a platform that combines hum...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9889345/ https://www.ncbi.nlm.nih.gov/pubmed/36720908 http://dx.doi.org/10.1038/s41598-023-28732-8 |
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author | Singh, Sameer Banerjee, Anoosha Vanden Broeck, Arnaud Klinge, Sebastian |
author_facet | Singh, Sameer Banerjee, Anoosha Vanden Broeck, Arnaud Klinge, Sebastian |
author_sort | Singh, Sameer |
collection | PubMed |
description | One of the challenges faced by current CRISPR/Cas9 editing strategies is the difficulty in rapidly selecting clonal populations of biallelically edited cells. Here we present Surface engiNeered fluorEscence Assisted Kit with Protein Epitope Enhanced Capture (SNEAK PEEC), a platform that combines human genome editing with cell-surface display, which enables the direct identification of biallelically edited clones with minimal screening. |
format | Online Article Text |
id | pubmed-9889345 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-98893452023-02-02 Rapid clonal identification of biallelic CRISPR/Cas9 knock-ins using SNEAK PEEC Singh, Sameer Banerjee, Anoosha Vanden Broeck, Arnaud Klinge, Sebastian Sci Rep Article One of the challenges faced by current CRISPR/Cas9 editing strategies is the difficulty in rapidly selecting clonal populations of biallelically edited cells. Here we present Surface engiNeered fluorEscence Assisted Kit with Protein Epitope Enhanced Capture (SNEAK PEEC), a platform that combines human genome editing with cell-surface display, which enables the direct identification of biallelically edited clones with minimal screening. Nature Publishing Group UK 2023-01-31 /pmc/articles/PMC9889345/ /pubmed/36720908 http://dx.doi.org/10.1038/s41598-023-28732-8 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Singh, Sameer Banerjee, Anoosha Vanden Broeck, Arnaud Klinge, Sebastian Rapid clonal identification of biallelic CRISPR/Cas9 knock-ins using SNEAK PEEC |
title | Rapid clonal identification of biallelic CRISPR/Cas9 knock-ins using SNEAK PEEC |
title_full | Rapid clonal identification of biallelic CRISPR/Cas9 knock-ins using SNEAK PEEC |
title_fullStr | Rapid clonal identification of biallelic CRISPR/Cas9 knock-ins using SNEAK PEEC |
title_full_unstemmed | Rapid clonal identification of biallelic CRISPR/Cas9 knock-ins using SNEAK PEEC |
title_short | Rapid clonal identification of biallelic CRISPR/Cas9 knock-ins using SNEAK PEEC |
title_sort | rapid clonal identification of biallelic crispr/cas9 knock-ins using sneak peec |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9889345/ https://www.ncbi.nlm.nih.gov/pubmed/36720908 http://dx.doi.org/10.1038/s41598-023-28732-8 |
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