Cargando…

Rapid clonal identification of biallelic CRISPR/Cas9 knock-ins using SNEAK PEEC

One of the challenges faced by current CRISPR/Cas9 editing strategies is the difficulty in rapidly selecting clonal populations of biallelically edited cells. Here we present Surface engiNeered fluorEscence Assisted Kit with Protein Epitope Enhanced Capture (SNEAK PEEC), a platform that combines hum...

Descripción completa

Detalles Bibliográficos
Autores principales: Singh, Sameer, Banerjee, Anoosha, Vanden Broeck, Arnaud, Klinge, Sebastian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9889345/
https://www.ncbi.nlm.nih.gov/pubmed/36720908
http://dx.doi.org/10.1038/s41598-023-28732-8
_version_ 1784880709732663296
author Singh, Sameer
Banerjee, Anoosha
Vanden Broeck, Arnaud
Klinge, Sebastian
author_facet Singh, Sameer
Banerjee, Anoosha
Vanden Broeck, Arnaud
Klinge, Sebastian
author_sort Singh, Sameer
collection PubMed
description One of the challenges faced by current CRISPR/Cas9 editing strategies is the difficulty in rapidly selecting clonal populations of biallelically edited cells. Here we present Surface engiNeered fluorEscence Assisted Kit with Protein Epitope Enhanced Capture (SNEAK PEEC), a platform that combines human genome editing with cell-surface display, which enables the direct identification of biallelically edited clones with minimal screening.
format Online
Article
Text
id pubmed-9889345
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-98893452023-02-02 Rapid clonal identification of biallelic CRISPR/Cas9 knock-ins using SNEAK PEEC Singh, Sameer Banerjee, Anoosha Vanden Broeck, Arnaud Klinge, Sebastian Sci Rep Article One of the challenges faced by current CRISPR/Cas9 editing strategies is the difficulty in rapidly selecting clonal populations of biallelically edited cells. Here we present Surface engiNeered fluorEscence Assisted Kit with Protein Epitope Enhanced Capture (SNEAK PEEC), a platform that combines human genome editing with cell-surface display, which enables the direct identification of biallelically edited clones with minimal screening. Nature Publishing Group UK 2023-01-31 /pmc/articles/PMC9889345/ /pubmed/36720908 http://dx.doi.org/10.1038/s41598-023-28732-8 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Singh, Sameer
Banerjee, Anoosha
Vanden Broeck, Arnaud
Klinge, Sebastian
Rapid clonal identification of biallelic CRISPR/Cas9 knock-ins using SNEAK PEEC
title Rapid clonal identification of biallelic CRISPR/Cas9 knock-ins using SNEAK PEEC
title_full Rapid clonal identification of biallelic CRISPR/Cas9 knock-ins using SNEAK PEEC
title_fullStr Rapid clonal identification of biallelic CRISPR/Cas9 knock-ins using SNEAK PEEC
title_full_unstemmed Rapid clonal identification of biallelic CRISPR/Cas9 knock-ins using SNEAK PEEC
title_short Rapid clonal identification of biallelic CRISPR/Cas9 knock-ins using SNEAK PEEC
title_sort rapid clonal identification of biallelic crispr/cas9 knock-ins using sneak peec
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9889345/
https://www.ncbi.nlm.nih.gov/pubmed/36720908
http://dx.doi.org/10.1038/s41598-023-28732-8
work_keys_str_mv AT singhsameer rapidclonalidentificationofbialleliccrisprcas9knockinsusingsneakpeec
AT banerjeeanoosha rapidclonalidentificationofbialleliccrisprcas9knockinsusingsneakpeec
AT vandenbroeckarnaud rapidclonalidentificationofbialleliccrisprcas9knockinsusingsneakpeec
AT klingesebastian rapidclonalidentificationofbialleliccrisprcas9knockinsusingsneakpeec