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A 50 Hz magnetic field influences the viability of breast cancer cells 96 h after exposure

BACKGROUND: The exposure of breast cancer to extremely low frequency magnetic fields (ELF-MFs) results in various biological responses. Some studies have suggested a possible cancer-enhancing effect, while others showed a possible therapeutic role. This study investigated the effects of in vitro exp...

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Autores principales: Elexpuru-Zabaleta, Maria, Lazzarini, Raffaella, Tartaglione, Maria Fiorella, Piva, Francesco, Ciarapica, Veronica, Marinelli Busilacchi, Elena, Poloni, Antonella, Valentino, Matteo, Santarelli, Lory, Bracci, Massimo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9889515/
https://www.ncbi.nlm.nih.gov/pubmed/36378418
http://dx.doi.org/10.1007/s11033-022-08069-7
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author Elexpuru-Zabaleta, Maria
Lazzarini, Raffaella
Tartaglione, Maria Fiorella
Piva, Francesco
Ciarapica, Veronica
Marinelli Busilacchi, Elena
Poloni, Antonella
Valentino, Matteo
Santarelli, Lory
Bracci, Massimo
author_facet Elexpuru-Zabaleta, Maria
Lazzarini, Raffaella
Tartaglione, Maria Fiorella
Piva, Francesco
Ciarapica, Veronica
Marinelli Busilacchi, Elena
Poloni, Antonella
Valentino, Matteo
Santarelli, Lory
Bracci, Massimo
author_sort Elexpuru-Zabaleta, Maria
collection PubMed
description BACKGROUND: The exposure of breast cancer to extremely low frequency magnetic fields (ELF-MFs) results in various biological responses. Some studies have suggested a possible cancer-enhancing effect, while others showed a possible therapeutic role. This study investigated the effects of in vitro exposure to 50 Hz ELF-MF for up to 24 h on the viability and cellular response of MDA-MB-231 and MCF-7 breast cancer cell lines and MCF-10A breast cell line. METHODS AND RESULTS: The breast cell lines were exposed to 50 Hz ELF-MF at flux densities of 0.1 mT and 1.0 mT and were examined 96 h after the beginning of ELF-MF exposure. The duration of 50 Hz ELF-MF exposure influenced the cell viability and proliferation of both the tumor and nontumorigenic breast cell lines. In particular, short-term exposure (4–8 h, 0.1 mT and 1.0 mT) led to an increase in viability in breast cancer cells, while long and high exposure (24 h, 1.0 mT) led to a decrease in viability and proliferation in all cell lines. Cancer and normal breast cells exhibited different responses to ELF-MF. Mitochondrial membrane potential and reactive oxygen species (ROS) production were altered after ELF-MF exposure, suggesting that the mitochondria are a probable target of ELF-MF in breast cells. CONCLUSIONS: The viability of breast cells in vitro is influenced by ELF-MF exposure at magnetic flux densities compatible with the limits for the general population and for workplace exposures. The effects are apparent after 96 h and are related to the ELF-MF exposure time. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11033-022-08069-7.
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spelling pubmed-98895152023-02-02 A 50 Hz magnetic field influences the viability of breast cancer cells 96 h after exposure Elexpuru-Zabaleta, Maria Lazzarini, Raffaella Tartaglione, Maria Fiorella Piva, Francesco Ciarapica, Veronica Marinelli Busilacchi, Elena Poloni, Antonella Valentino, Matteo Santarelli, Lory Bracci, Massimo Mol Biol Rep Original Article BACKGROUND: The exposure of breast cancer to extremely low frequency magnetic fields (ELF-MFs) results in various biological responses. Some studies have suggested a possible cancer-enhancing effect, while others showed a possible therapeutic role. This study investigated the effects of in vitro exposure to 50 Hz ELF-MF for up to 24 h on the viability and cellular response of MDA-MB-231 and MCF-7 breast cancer cell lines and MCF-10A breast cell line. METHODS AND RESULTS: The breast cell lines were exposed to 50 Hz ELF-MF at flux densities of 0.1 mT and 1.0 mT and were examined 96 h after the beginning of ELF-MF exposure. The duration of 50 Hz ELF-MF exposure influenced the cell viability and proliferation of both the tumor and nontumorigenic breast cell lines. In particular, short-term exposure (4–8 h, 0.1 mT and 1.0 mT) led to an increase in viability in breast cancer cells, while long and high exposure (24 h, 1.0 mT) led to a decrease in viability and proliferation in all cell lines. Cancer and normal breast cells exhibited different responses to ELF-MF. Mitochondrial membrane potential and reactive oxygen species (ROS) production were altered after ELF-MF exposure, suggesting that the mitochondria are a probable target of ELF-MF in breast cells. CONCLUSIONS: The viability of breast cells in vitro is influenced by ELF-MF exposure at magnetic flux densities compatible with the limits for the general population and for workplace exposures. The effects are apparent after 96 h and are related to the ELF-MF exposure time. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11033-022-08069-7. Springer Netherlands 2022-11-15 2023 /pmc/articles/PMC9889515/ /pubmed/36378418 http://dx.doi.org/10.1007/s11033-022-08069-7 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Article
Elexpuru-Zabaleta, Maria
Lazzarini, Raffaella
Tartaglione, Maria Fiorella
Piva, Francesco
Ciarapica, Veronica
Marinelli Busilacchi, Elena
Poloni, Antonella
Valentino, Matteo
Santarelli, Lory
Bracci, Massimo
A 50 Hz magnetic field influences the viability of breast cancer cells 96 h after exposure
title A 50 Hz magnetic field influences the viability of breast cancer cells 96 h after exposure
title_full A 50 Hz magnetic field influences the viability of breast cancer cells 96 h after exposure
title_fullStr A 50 Hz magnetic field influences the viability of breast cancer cells 96 h after exposure
title_full_unstemmed A 50 Hz magnetic field influences the viability of breast cancer cells 96 h after exposure
title_short A 50 Hz magnetic field influences the viability of breast cancer cells 96 h after exposure
title_sort 50 hz magnetic field influences the viability of breast cancer cells 96 h after exposure
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9889515/
https://www.ncbi.nlm.nih.gov/pubmed/36378418
http://dx.doi.org/10.1007/s11033-022-08069-7
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