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Relieving your stress: PGPB associated with Andean xerophytic plants are most abundant and active on the most extreme slopes

INTRODUCTION: Plants interact with plant growth-promoting bacteria (PGPB), especially under stress condition in natural and agricultural systems. Although a potentially beneficial microbiome has been found associated to plants from alpine systems, this plant- PGPB interaction has been scarcely studi...

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Autores principales: Aguilera-Torres, Carla, Riveros, Gustavo, Morales, Loreto V., Sierra-Almeida, Angela, Schoebitz, Mauricio, Hasbún, Rodrigo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9889642/
https://www.ncbi.nlm.nih.gov/pubmed/36741893
http://dx.doi.org/10.3389/fmicb.2022.1062414
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author Aguilera-Torres, Carla
Riveros, Gustavo
Morales, Loreto V.
Sierra-Almeida, Angela
Schoebitz, Mauricio
Hasbún, Rodrigo
author_facet Aguilera-Torres, Carla
Riveros, Gustavo
Morales, Loreto V.
Sierra-Almeida, Angela
Schoebitz, Mauricio
Hasbún, Rodrigo
author_sort Aguilera-Torres, Carla
collection PubMed
description INTRODUCTION: Plants interact with plant growth-promoting bacteria (PGPB), especially under stress condition in natural and agricultural systems. Although a potentially beneficial microbiome has been found associated to plants from alpine systems, this plant- PGPB interaction has been scarcely studied. Nevados de Chillán Complex hold one of the southernmost xerophytic formations in Chile. Plant species living there have to cope with drought and extreme temperatures during the growing season period, microclimatic conditions that become harsher on equatorial than polar slopes, and where the interaction with PGPB could be key for plant survival. Our goal was to study the abundance and activity of different PGPB associated to two abundant plant species of Andean xerophytic formations on contrasting slopes. METHODS: Twenty individuals of Berberis empetrifolia and Azorella prolifera shrubs were selected growing on a north and south slope nearby Las Fumarolas, at 2,050 m elevation. On each slope, microclimate based on temperature and moisture conditions were monitored throughout the growing period (oct. – apr.). Chemical properties of the soil under plant species canopies were also characterized. Bacterial abundance was measured as Log CFU g(−1) from soil samples collected from each individual and slope. Then, the most abundant bacterial colonies were selected, and different hormonal (indoleacetic acid) and enzymatic (nitrogenase, phosphatase, ACC-deaminase) mechanisms that promote plant growth were assessed and measured. RESULTS AND DISCUSSION: Extreme temperatures were observed in the north facing slope, recording the hottest days (41 vs. 36°C) and coldest nights (−9.9 vs. 6.6°C). Moreover, air and soil moisture were lower on north than on south slope, especially late in the growing season. We found that bacterial abundance was higher in soils on north than on south slope but only under B. empetrifolia canopy. Moreover, the activity of plant growth-promoting mechanisms varied between slopes, being on average higher on north than on south slope, but with plant species-dependent trends. Our work showed how the environmental heterogeneity at microscale in alpine systems (slope and plant species identity) underlies variations in the abundance and plant growth promoting activity of the microorganisms present under the plant canopy of the Andean xerophytic formations and highlight the importance of PGPB from harsh systems as biotechnological tools for restoration.
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spelling pubmed-98896422023-02-02 Relieving your stress: PGPB associated with Andean xerophytic plants are most abundant and active on the most extreme slopes Aguilera-Torres, Carla Riveros, Gustavo Morales, Loreto V. Sierra-Almeida, Angela Schoebitz, Mauricio Hasbún, Rodrigo Front Microbiol Microbiology INTRODUCTION: Plants interact with plant growth-promoting bacteria (PGPB), especially under stress condition in natural and agricultural systems. Although a potentially beneficial microbiome has been found associated to plants from alpine systems, this plant- PGPB interaction has been scarcely studied. Nevados de Chillán Complex hold one of the southernmost xerophytic formations in Chile. Plant species living there have to cope with drought and extreme temperatures during the growing season period, microclimatic conditions that become harsher on equatorial than polar slopes, and where the interaction with PGPB could be key for plant survival. Our goal was to study the abundance and activity of different PGPB associated to two abundant plant species of Andean xerophytic formations on contrasting slopes. METHODS: Twenty individuals of Berberis empetrifolia and Azorella prolifera shrubs were selected growing on a north and south slope nearby Las Fumarolas, at 2,050 m elevation. On each slope, microclimate based on temperature and moisture conditions were monitored throughout the growing period (oct. – apr.). Chemical properties of the soil under plant species canopies were also characterized. Bacterial abundance was measured as Log CFU g(−1) from soil samples collected from each individual and slope. Then, the most abundant bacterial colonies were selected, and different hormonal (indoleacetic acid) and enzymatic (nitrogenase, phosphatase, ACC-deaminase) mechanisms that promote plant growth were assessed and measured. RESULTS AND DISCUSSION: Extreme temperatures were observed in the north facing slope, recording the hottest days (41 vs. 36°C) and coldest nights (−9.9 vs. 6.6°C). Moreover, air and soil moisture were lower on north than on south slope, especially late in the growing season. We found that bacterial abundance was higher in soils on north than on south slope but only under B. empetrifolia canopy. Moreover, the activity of plant growth-promoting mechanisms varied between slopes, being on average higher on north than on south slope, but with plant species-dependent trends. Our work showed how the environmental heterogeneity at microscale in alpine systems (slope and plant species identity) underlies variations in the abundance and plant growth promoting activity of the microorganisms present under the plant canopy of the Andean xerophytic formations and highlight the importance of PGPB from harsh systems as biotechnological tools for restoration. Frontiers Media S.A. 2023-01-18 /pmc/articles/PMC9889642/ /pubmed/36741893 http://dx.doi.org/10.3389/fmicb.2022.1062414 Text en Copyright © 2023 Aguilera-Torres, Riveros, Morales, Sierra-Almeida, Schoebitz and Hasbún. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Aguilera-Torres, Carla
Riveros, Gustavo
Morales, Loreto V.
Sierra-Almeida, Angela
Schoebitz, Mauricio
Hasbún, Rodrigo
Relieving your stress: PGPB associated with Andean xerophytic plants are most abundant and active on the most extreme slopes
title Relieving your stress: PGPB associated with Andean xerophytic plants are most abundant and active on the most extreme slopes
title_full Relieving your stress: PGPB associated with Andean xerophytic plants are most abundant and active on the most extreme slopes
title_fullStr Relieving your stress: PGPB associated with Andean xerophytic plants are most abundant and active on the most extreme slopes
title_full_unstemmed Relieving your stress: PGPB associated with Andean xerophytic plants are most abundant and active on the most extreme slopes
title_short Relieving your stress: PGPB associated with Andean xerophytic plants are most abundant and active on the most extreme slopes
title_sort relieving your stress: pgpb associated with andean xerophytic plants are most abundant and active on the most extreme slopes
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9889642/
https://www.ncbi.nlm.nih.gov/pubmed/36741893
http://dx.doi.org/10.3389/fmicb.2022.1062414
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