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Translational regulation by Hfq–Crc assemblies emerges from polymorphic ribonucleoprotein folding

The widely occurring bacterial RNA chaperone Hfq is a key factor in the post‐transcriptional control of hundreds of genes in Pseudomonas aeruginosa. How this broadly acting protein can contribute to the regulatory requirements of many different genes remains puzzling. Here, we describe cryo‐EM struc...

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Autores principales: Dendooven, Tom, Sonnleitner, Elisabeth, Bläsi, Udo, Luisi, Ben F
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9890229/
https://www.ncbi.nlm.nih.gov/pubmed/36504222
http://dx.doi.org/10.15252/embj.2022111129
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author Dendooven, Tom
Sonnleitner, Elisabeth
Bläsi, Udo
Luisi, Ben F
author_facet Dendooven, Tom
Sonnleitner, Elisabeth
Bläsi, Udo
Luisi, Ben F
author_sort Dendooven, Tom
collection PubMed
description The widely occurring bacterial RNA chaperone Hfq is a key factor in the post‐transcriptional control of hundreds of genes in Pseudomonas aeruginosa. How this broadly acting protein can contribute to the regulatory requirements of many different genes remains puzzling. Here, we describe cryo‐EM structures of higher order assemblies formed by Hfq and its partner protein Crc on control regions of different P. aeruginosa target mRNAs. Our results show that these assemblies have mRNA‐specific quaternary architectures resulting from the combination of multivalent protein–protein interfaces and recognition of patterns in the RNA sequence. The structural polymorphism of these ribonucleoprotein assemblies enables selective translational repression of many different target mRNAs. This system elucidates how highly complex regulatory pathways can evolve with a minimal economy of proteinogenic components in combination with RNA sequence and fold.
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spelling pubmed-98902292023-02-09 Translational regulation by Hfq–Crc assemblies emerges from polymorphic ribonucleoprotein folding Dendooven, Tom Sonnleitner, Elisabeth Bläsi, Udo Luisi, Ben F EMBO J Articles The widely occurring bacterial RNA chaperone Hfq is a key factor in the post‐transcriptional control of hundreds of genes in Pseudomonas aeruginosa. How this broadly acting protein can contribute to the regulatory requirements of many different genes remains puzzling. Here, we describe cryo‐EM structures of higher order assemblies formed by Hfq and its partner protein Crc on control regions of different P. aeruginosa target mRNAs. Our results show that these assemblies have mRNA‐specific quaternary architectures resulting from the combination of multivalent protein–protein interfaces and recognition of patterns in the RNA sequence. The structural polymorphism of these ribonucleoprotein assemblies enables selective translational repression of many different target mRNAs. This system elucidates how highly complex regulatory pathways can evolve with a minimal economy of proteinogenic components in combination with RNA sequence and fold. John Wiley and Sons Inc. 2022-12-12 /pmc/articles/PMC9890229/ /pubmed/36504222 http://dx.doi.org/10.15252/embj.2022111129 Text en © 2022 The Authors. Published under the terms of the CC BY 4.0 license. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Articles
Dendooven, Tom
Sonnleitner, Elisabeth
Bläsi, Udo
Luisi, Ben F
Translational regulation by Hfq–Crc assemblies emerges from polymorphic ribonucleoprotein folding
title Translational regulation by Hfq–Crc assemblies emerges from polymorphic ribonucleoprotein folding
title_full Translational regulation by Hfq–Crc assemblies emerges from polymorphic ribonucleoprotein folding
title_fullStr Translational regulation by Hfq–Crc assemblies emerges from polymorphic ribonucleoprotein folding
title_full_unstemmed Translational regulation by Hfq–Crc assemblies emerges from polymorphic ribonucleoprotein folding
title_short Translational regulation by Hfq–Crc assemblies emerges from polymorphic ribonucleoprotein folding
title_sort translational regulation by hfq–crc assemblies emerges from polymorphic ribonucleoprotein folding
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9890229/
https://www.ncbi.nlm.nih.gov/pubmed/36504222
http://dx.doi.org/10.15252/embj.2022111129
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