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Apolipoprotein H: a novel regulator of fat accumulation in duck myoblasts

Apolipoprotein H (APOH) primarily engages in fat metabolism and inflammatory disease response. This study aimed to investigate the effects of APOH on fat synthesis in duck myoblasts (CS2s) by APOH overexpression and knockdown. CS2s overexpressing APOH showed enhanced triglyceride (TG) and cholestero...

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Autores principales: Pan, Ziyi, Du, Guoqing, Li, Guoyu, Wu, Dongsheng, Chen, Xingyong, Geng, Zhaoyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society of Animal Sciences and Technology 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9890340/
https://www.ncbi.nlm.nih.gov/pubmed/36812035
http://dx.doi.org/10.5187/jast.2022.e60
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author Pan, Ziyi
Du, Guoqing
Li, Guoyu
Wu, Dongsheng
Chen, Xingyong
Geng, Zhaoyu
author_facet Pan, Ziyi
Du, Guoqing
Li, Guoyu
Wu, Dongsheng
Chen, Xingyong
Geng, Zhaoyu
author_sort Pan, Ziyi
collection PubMed
description Apolipoprotein H (APOH) primarily engages in fat metabolism and inflammatory disease response. This study aimed to investigate the effects of APOH on fat synthesis in duck myoblasts (CS2s) by APOH overexpression and knockdown. CS2s overexpressing APOH showed enhanced triglyceride (TG) and cholesterol (CHOL) contents and elevated the mRNA and protein expression of AKT serine/threonine kinase 1 (AKT1), ELOVL fatty acid elongase 6 (ELOVL6), and acetyl-CoA carboxylase 1 (ACC1) while reducing the expression of protein kinase AMP-activated catalytic subunit alpha 1 (AMPK), peroxisome proliferator activated receptor gamma (PPARG), acyl-CoA synthetase long chain family member 1 (ACSL1), and lipoprotein lipase (LPL). The results showed that knockdown of APOH in CS2s reduced the content of TG and CHOL, reduced the expression of ACC1, ELOVL6, and AKT1, and increased the gene and protein expression of PPARG, LPL, ACSL1, and AMPK. Our results showed that APOH affected lipid deposition in myoblasts by inhibiting fatty acid beta-oxidation and promoting fatty acid biosynthesis by regulating the expression of the AKT/AMPK pathway. This study provides the necessary basic information for the role of APOH in fat accumulation in duck myoblasts for the first time and enables researchers to study the genes related to fat deposition in meat ducks in a new direction.
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spelling pubmed-98903402023-02-14 Apolipoprotein H: a novel regulator of fat accumulation in duck myoblasts Pan, Ziyi Du, Guoqing Li, Guoyu Wu, Dongsheng Chen, Xingyong Geng, Zhaoyu J Anim Sci Technol Research Article Apolipoprotein H (APOH) primarily engages in fat metabolism and inflammatory disease response. This study aimed to investigate the effects of APOH on fat synthesis in duck myoblasts (CS2s) by APOH overexpression and knockdown. CS2s overexpressing APOH showed enhanced triglyceride (TG) and cholesterol (CHOL) contents and elevated the mRNA and protein expression of AKT serine/threonine kinase 1 (AKT1), ELOVL fatty acid elongase 6 (ELOVL6), and acetyl-CoA carboxylase 1 (ACC1) while reducing the expression of protein kinase AMP-activated catalytic subunit alpha 1 (AMPK), peroxisome proliferator activated receptor gamma (PPARG), acyl-CoA synthetase long chain family member 1 (ACSL1), and lipoprotein lipase (LPL). The results showed that knockdown of APOH in CS2s reduced the content of TG and CHOL, reduced the expression of ACC1, ELOVL6, and AKT1, and increased the gene and protein expression of PPARG, LPL, ACSL1, and AMPK. Our results showed that APOH affected lipid deposition in myoblasts by inhibiting fatty acid beta-oxidation and promoting fatty acid biosynthesis by regulating the expression of the AKT/AMPK pathway. This study provides the necessary basic information for the role of APOH in fat accumulation in duck myoblasts for the first time and enables researchers to study the genes related to fat deposition in meat ducks in a new direction. Korean Society of Animal Sciences and Technology 2022-11 2022-11-30 /pmc/articles/PMC9890340/ /pubmed/36812035 http://dx.doi.org/10.5187/jast.2022.e60 Text en © Copyright 2022 Korean Society of Animal Science and Technology https://creativecommons.org/licenses/by-nc/4.0/This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Pan, Ziyi
Du, Guoqing
Li, Guoyu
Wu, Dongsheng
Chen, Xingyong
Geng, Zhaoyu
Apolipoprotein H: a novel regulator of fat accumulation in duck myoblasts
title Apolipoprotein H: a novel regulator of fat accumulation in duck myoblasts
title_full Apolipoprotein H: a novel regulator of fat accumulation in duck myoblasts
title_fullStr Apolipoprotein H: a novel regulator of fat accumulation in duck myoblasts
title_full_unstemmed Apolipoprotein H: a novel regulator of fat accumulation in duck myoblasts
title_short Apolipoprotein H: a novel regulator of fat accumulation in duck myoblasts
title_sort apolipoprotein h: a novel regulator of fat accumulation in duck myoblasts
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9890340/
https://www.ncbi.nlm.nih.gov/pubmed/36812035
http://dx.doi.org/10.5187/jast.2022.e60
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