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Genome-Based Characterization of Multidrug-Resistant Escherichia coli Isolated from Clinical Bovine Mastitis
Mastitis occurrence in dairy cows is a broad topic that involves several sectors, from antimicrobial resistance and virulence of strains to economic implications and cattle management practices. Here, we assessed the molecular characterization (antimicrobial resistance determinants, virulence genes,...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9890429/ https://www.ncbi.nlm.nih.gov/pubmed/36723699 http://dx.doi.org/10.1007/s00284-023-03191-6 |
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author | dos Santos Alves, Taila Rosa, Vinícius Sanches da Silva Leite, Domingos Guerra, Simony Trevizan Joaquim, Sâmea Fernandes Guimarães, Felipe Freitas de Figueiredo Pantoja, José Carlos Lucheis, Simoni Baldini Rall, Vera Lúcia Mores Hernandes, Rodrigo Tavanelli Langoni, Helio Ribeiro, Márcio Garcia |
author_facet | dos Santos Alves, Taila Rosa, Vinícius Sanches da Silva Leite, Domingos Guerra, Simony Trevizan Joaquim, Sâmea Fernandes Guimarães, Felipe Freitas de Figueiredo Pantoja, José Carlos Lucheis, Simoni Baldini Rall, Vera Lúcia Mores Hernandes, Rodrigo Tavanelli Langoni, Helio Ribeiro, Márcio Garcia |
author_sort | dos Santos Alves, Taila |
collection | PubMed |
description | Mastitis occurrence in dairy cows is a broad topic that involves several sectors, from antimicrobial resistance and virulence of strains to economic implications and cattle management practices. Here, we assessed the molecular characterization (antimicrobial resistance determinants, virulence genes, sequences type, serotypes, and plasmid types) of 178 Escherichia coli strains isolated from milk samples from cows with clinical mastitis using a genome-based k-mers approach. Of these, 53 (29.8%) showed multidrug resistance by disc diffusion. We selected eight multidrug-resistant mastitis-associated E. coli for whole-genome sequencing and molecular characterization based on raw data using k-mers. We assessed antimicrobial resistance genes, virulence factors, serotypes, Multilocus Sequence Typing (MLST), and plasmid types. The most antimicrobial resistance gene found were bla(TEM-1B) (7/8), tetA (6/8), strA (6/8), strB (6/8), and qnrB19 (5/8). A total of 25 virulence factors were detected encoding adhesins, capsule, enzymes/proteins, increased serum survival, hemolysin, colicins, and iron uptake. These virulence factors were associated with Extraintestinal Pathogenic E. coli. Three pandemic clones were found: ST10, ST101, and ST69. Two E. coli were assigned in the O117 serogroup and one in the O8:H25 serotype. The most common plasmid groups were IncFII (7/8) and IncFIB (6/8). Our findings contribute to the knowledge of virulence mechanisms, epidemiological aspects, and antimicrobial resistance determinants of E. coli strains obtained from clinical mammary infections of cows. |
format | Online Article Text |
id | pubmed-9890429 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-98904292023-02-01 Genome-Based Characterization of Multidrug-Resistant Escherichia coli Isolated from Clinical Bovine Mastitis dos Santos Alves, Taila Rosa, Vinícius Sanches da Silva Leite, Domingos Guerra, Simony Trevizan Joaquim, Sâmea Fernandes Guimarães, Felipe Freitas de Figueiredo Pantoja, José Carlos Lucheis, Simoni Baldini Rall, Vera Lúcia Mores Hernandes, Rodrigo Tavanelli Langoni, Helio Ribeiro, Márcio Garcia Curr Microbiol Short Communication Mastitis occurrence in dairy cows is a broad topic that involves several sectors, from antimicrobial resistance and virulence of strains to economic implications and cattle management practices. Here, we assessed the molecular characterization (antimicrobial resistance determinants, virulence genes, sequences type, serotypes, and plasmid types) of 178 Escherichia coli strains isolated from milk samples from cows with clinical mastitis using a genome-based k-mers approach. Of these, 53 (29.8%) showed multidrug resistance by disc diffusion. We selected eight multidrug-resistant mastitis-associated E. coli for whole-genome sequencing and molecular characterization based on raw data using k-mers. We assessed antimicrobial resistance genes, virulence factors, serotypes, Multilocus Sequence Typing (MLST), and plasmid types. The most antimicrobial resistance gene found were bla(TEM-1B) (7/8), tetA (6/8), strA (6/8), strB (6/8), and qnrB19 (5/8). A total of 25 virulence factors were detected encoding adhesins, capsule, enzymes/proteins, increased serum survival, hemolysin, colicins, and iron uptake. These virulence factors were associated with Extraintestinal Pathogenic E. coli. Three pandemic clones were found: ST10, ST101, and ST69. Two E. coli were assigned in the O117 serogroup and one in the O8:H25 serotype. The most common plasmid groups were IncFII (7/8) and IncFIB (6/8). Our findings contribute to the knowledge of virulence mechanisms, epidemiological aspects, and antimicrobial resistance determinants of E. coli strains obtained from clinical mammary infections of cows. Springer US 2023-02-01 2023 /pmc/articles/PMC9890429/ /pubmed/36723699 http://dx.doi.org/10.1007/s00284-023-03191-6 Text en © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2023 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Short Communication dos Santos Alves, Taila Rosa, Vinícius Sanches da Silva Leite, Domingos Guerra, Simony Trevizan Joaquim, Sâmea Fernandes Guimarães, Felipe Freitas de Figueiredo Pantoja, José Carlos Lucheis, Simoni Baldini Rall, Vera Lúcia Mores Hernandes, Rodrigo Tavanelli Langoni, Helio Ribeiro, Márcio Garcia Genome-Based Characterization of Multidrug-Resistant Escherichia coli Isolated from Clinical Bovine Mastitis |
title | Genome-Based Characterization of Multidrug-Resistant Escherichia coli Isolated from Clinical Bovine Mastitis |
title_full | Genome-Based Characterization of Multidrug-Resistant Escherichia coli Isolated from Clinical Bovine Mastitis |
title_fullStr | Genome-Based Characterization of Multidrug-Resistant Escherichia coli Isolated from Clinical Bovine Mastitis |
title_full_unstemmed | Genome-Based Characterization of Multidrug-Resistant Escherichia coli Isolated from Clinical Bovine Mastitis |
title_short | Genome-Based Characterization of Multidrug-Resistant Escherichia coli Isolated from Clinical Bovine Mastitis |
title_sort | genome-based characterization of multidrug-resistant escherichia coli isolated from clinical bovine mastitis |
topic | Short Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9890429/ https://www.ncbi.nlm.nih.gov/pubmed/36723699 http://dx.doi.org/10.1007/s00284-023-03191-6 |
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