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Laser-assisted protein micropatterning in a thermoplastic device for multiplexed prostate cancer biomarker detection

Immunoassays are frequently used for analysis of protein biomarkers. The specificity of antibodies enables parallel analysis of several target proteins, at the same time. However, the implementation of such multiplexed assays into cost-efficient and mass-producible thermoplastic microfluidic platfor...

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Autores principales: Kling, André, Dirscherl, Lorin, Dittrich, Petra S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9890490/
https://www.ncbi.nlm.nih.gov/pubmed/36642981
http://dx.doi.org/10.1039/d2lc00840h
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author Kling, André
Dirscherl, Lorin
Dittrich, Petra S.
author_facet Kling, André
Dirscherl, Lorin
Dittrich, Petra S.
author_sort Kling, André
collection PubMed
description Immunoassays are frequently used for analysis of protein biomarkers. The specificity of antibodies enables parallel analysis of several target proteins, at the same time. However, the implementation of such multiplexed assays into cost-efficient and mass-producible thermoplastic microfluidic platforms remains difficult due to the lack of suitable immobilization strategies for different capture antibodies. Here, we introduce and characterize a method to functionalize the surfaces of microfluidic devices manufactured in the thermoplastic material cyclic olefin copolymer (COC) by a rapid prototyping process. A laser-induced immobilization process enables the surface patterning of anchor biomolecules at a spatial resolution of 5 μm. We employ the method for the analysis of prostate cancer associated biomarkers by competitive immunoassays in a microchannel with a total volume of 320 nL, and successfully detected the proteins PSA, CRP, CEA and IGF-1 at clinically relevant concentrations. Finally, we also demonstrate the simultaneous analysis of three markers spiked into undiluted human plasma. In conclusion, this method opens the way to transfer multiplexed immunoassays into mass-producible microfluidic platforms that are suitable for point of care applications.
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spelling pubmed-98904902023-02-02 Laser-assisted protein micropatterning in a thermoplastic device for multiplexed prostate cancer biomarker detection Kling, André Dirscherl, Lorin Dittrich, Petra S. Lab Chip Chemistry Immunoassays are frequently used for analysis of protein biomarkers. The specificity of antibodies enables parallel analysis of several target proteins, at the same time. However, the implementation of such multiplexed assays into cost-efficient and mass-producible thermoplastic microfluidic platforms remains difficult due to the lack of suitable immobilization strategies for different capture antibodies. Here, we introduce and characterize a method to functionalize the surfaces of microfluidic devices manufactured in the thermoplastic material cyclic olefin copolymer (COC) by a rapid prototyping process. A laser-induced immobilization process enables the surface patterning of anchor biomolecules at a spatial resolution of 5 μm. We employ the method for the analysis of prostate cancer associated biomarkers by competitive immunoassays in a microchannel with a total volume of 320 nL, and successfully detected the proteins PSA, CRP, CEA and IGF-1 at clinically relevant concentrations. Finally, we also demonstrate the simultaneous analysis of three markers spiked into undiluted human plasma. In conclusion, this method opens the way to transfer multiplexed immunoassays into mass-producible microfluidic platforms that are suitable for point of care applications. The Royal Society of Chemistry 2023-01-04 /pmc/articles/PMC9890490/ /pubmed/36642981 http://dx.doi.org/10.1039/d2lc00840h Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Kling, André
Dirscherl, Lorin
Dittrich, Petra S.
Laser-assisted protein micropatterning in a thermoplastic device for multiplexed prostate cancer biomarker detection
title Laser-assisted protein micropatterning in a thermoplastic device for multiplexed prostate cancer biomarker detection
title_full Laser-assisted protein micropatterning in a thermoplastic device for multiplexed prostate cancer biomarker detection
title_fullStr Laser-assisted protein micropatterning in a thermoplastic device for multiplexed prostate cancer biomarker detection
title_full_unstemmed Laser-assisted protein micropatterning in a thermoplastic device for multiplexed prostate cancer biomarker detection
title_short Laser-assisted protein micropatterning in a thermoplastic device for multiplexed prostate cancer biomarker detection
title_sort laser-assisted protein micropatterning in a thermoplastic device for multiplexed prostate cancer biomarker detection
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9890490/
https://www.ncbi.nlm.nih.gov/pubmed/36642981
http://dx.doi.org/10.1039/d2lc00840h
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