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Laser-assisted protein micropatterning in a thermoplastic device for multiplexed prostate cancer biomarker detection
Immunoassays are frequently used for analysis of protein biomarkers. The specificity of antibodies enables parallel analysis of several target proteins, at the same time. However, the implementation of such multiplexed assays into cost-efficient and mass-producible thermoplastic microfluidic platfor...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9890490/ https://www.ncbi.nlm.nih.gov/pubmed/36642981 http://dx.doi.org/10.1039/d2lc00840h |
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author | Kling, André Dirscherl, Lorin Dittrich, Petra S. |
author_facet | Kling, André Dirscherl, Lorin Dittrich, Petra S. |
author_sort | Kling, André |
collection | PubMed |
description | Immunoassays are frequently used for analysis of protein biomarkers. The specificity of antibodies enables parallel analysis of several target proteins, at the same time. However, the implementation of such multiplexed assays into cost-efficient and mass-producible thermoplastic microfluidic platforms remains difficult due to the lack of suitable immobilization strategies for different capture antibodies. Here, we introduce and characterize a method to functionalize the surfaces of microfluidic devices manufactured in the thermoplastic material cyclic olefin copolymer (COC) by a rapid prototyping process. A laser-induced immobilization process enables the surface patterning of anchor biomolecules at a spatial resolution of 5 μm. We employ the method for the analysis of prostate cancer associated biomarkers by competitive immunoassays in a microchannel with a total volume of 320 nL, and successfully detected the proteins PSA, CRP, CEA and IGF-1 at clinically relevant concentrations. Finally, we also demonstrate the simultaneous analysis of three markers spiked into undiluted human plasma. In conclusion, this method opens the way to transfer multiplexed immunoassays into mass-producible microfluidic platforms that are suitable for point of care applications. |
format | Online Article Text |
id | pubmed-9890490 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-98904902023-02-02 Laser-assisted protein micropatterning in a thermoplastic device for multiplexed prostate cancer biomarker detection Kling, André Dirscherl, Lorin Dittrich, Petra S. Lab Chip Chemistry Immunoassays are frequently used for analysis of protein biomarkers. The specificity of antibodies enables parallel analysis of several target proteins, at the same time. However, the implementation of such multiplexed assays into cost-efficient and mass-producible thermoplastic microfluidic platforms remains difficult due to the lack of suitable immobilization strategies for different capture antibodies. Here, we introduce and characterize a method to functionalize the surfaces of microfluidic devices manufactured in the thermoplastic material cyclic olefin copolymer (COC) by a rapid prototyping process. A laser-induced immobilization process enables the surface patterning of anchor biomolecules at a spatial resolution of 5 μm. We employ the method for the analysis of prostate cancer associated biomarkers by competitive immunoassays in a microchannel with a total volume of 320 nL, and successfully detected the proteins PSA, CRP, CEA and IGF-1 at clinically relevant concentrations. Finally, we also demonstrate the simultaneous analysis of three markers spiked into undiluted human plasma. In conclusion, this method opens the way to transfer multiplexed immunoassays into mass-producible microfluidic platforms that are suitable for point of care applications. The Royal Society of Chemistry 2023-01-04 /pmc/articles/PMC9890490/ /pubmed/36642981 http://dx.doi.org/10.1039/d2lc00840h Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/ |
spellingShingle | Chemistry Kling, André Dirscherl, Lorin Dittrich, Petra S. Laser-assisted protein micropatterning in a thermoplastic device for multiplexed prostate cancer biomarker detection |
title | Laser-assisted protein micropatterning in a thermoplastic device for multiplexed prostate cancer biomarker detection |
title_full | Laser-assisted protein micropatterning in a thermoplastic device for multiplexed prostate cancer biomarker detection |
title_fullStr | Laser-assisted protein micropatterning in a thermoplastic device for multiplexed prostate cancer biomarker detection |
title_full_unstemmed | Laser-assisted protein micropatterning in a thermoplastic device for multiplexed prostate cancer biomarker detection |
title_short | Laser-assisted protein micropatterning in a thermoplastic device for multiplexed prostate cancer biomarker detection |
title_sort | laser-assisted protein micropatterning in a thermoplastic device for multiplexed prostate cancer biomarker detection |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9890490/ https://www.ncbi.nlm.nih.gov/pubmed/36642981 http://dx.doi.org/10.1039/d2lc00840h |
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