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ATF7‐dependent epigenetic changes induced by high temperature during early porcine embryonic development
BACKGROUND: Activating transcription factor 7 (ATF7) is a member of the ATF/cAMP response element (CRE) B superfamily. ATF2, ATF7, and CRE‐BPa are present in vertebrates. Drosophila and fission yeast have only one homologue: dATF2 and Atf1, respectively. Under normal conditions, ATF7 promotes hetero...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9890523/ https://www.ncbi.nlm.nih.gov/pubmed/36254813 http://dx.doi.org/10.1111/cpr.13352 |
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author | Sun, Ming‐Hong Jiang, Wen‐Jie Li, Xiao‐Han Lee, Song‐Hee Heo, Geun Zhou, Dongjie Choi, Jung‐Seok Kim, Kwan‐Suk Lv, Wenfa Cui, Xiang‐Shun |
author_facet | Sun, Ming‐Hong Jiang, Wen‐Jie Li, Xiao‐Han Lee, Song‐Hee Heo, Geun Zhou, Dongjie Choi, Jung‐Seok Kim, Kwan‐Suk Lv, Wenfa Cui, Xiang‐Shun |
author_sort | Sun, Ming‐Hong |
collection | PubMed |
description | BACKGROUND: Activating transcription factor 7 (ATF7) is a member of the ATF/cAMP response element (CRE) B superfamily. ATF2, ATF7, and CRE‐BPa are present in vertebrates. Drosophila and fission yeast have only one homologue: dATF2 and Atf1, respectively. Under normal conditions, ATF7 promotes heterochromatin formation by recruiting histone H3K9 di‐ and tri‐methyltransferases. Once the situation changes, all members are phosphorylated by the stress‐activated kinase P38 in response to various stressors. However, the role of ATF7 in early porcine embryonic development remains unclear. RESULTS: In this study, we found that ATF7 gradually accumulated in the nucleus and then localized on the pericentric heterochromatin after the late 4‐cell stage, while being co‐localized with heterochromatin protein 1 (HP1). Knockdown of ATF7 resulted in decreases in the blastocyst rate and blastocyst cell number. ATF7 depletion resulted in downregulation of HP1 and histone 3 lysine 9 dimethylation (H3K9me2) expression. These effects were alleviated when P38 activity was inhibited. High temperatures increased the expression level of pP38, while reducing the quality of porcine embryos, and led to ATF7 phosphorylation. The expression level of H3K9me2 and HP1 was decreased and regulated by P38 activity. CONCLUSION: Stress‐induced ATF7‐dependent epigenetic changes play important roles in early porcine embryonic development. |
format | Online Article Text |
id | pubmed-9890523 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-98905232023-02-02 ATF7‐dependent epigenetic changes induced by high temperature during early porcine embryonic development Sun, Ming‐Hong Jiang, Wen‐Jie Li, Xiao‐Han Lee, Song‐Hee Heo, Geun Zhou, Dongjie Choi, Jung‐Seok Kim, Kwan‐Suk Lv, Wenfa Cui, Xiang‐Shun Cell Prolif Original Articles BACKGROUND: Activating transcription factor 7 (ATF7) is a member of the ATF/cAMP response element (CRE) B superfamily. ATF2, ATF7, and CRE‐BPa are present in vertebrates. Drosophila and fission yeast have only one homologue: dATF2 and Atf1, respectively. Under normal conditions, ATF7 promotes heterochromatin formation by recruiting histone H3K9 di‐ and tri‐methyltransferases. Once the situation changes, all members are phosphorylated by the stress‐activated kinase P38 in response to various stressors. However, the role of ATF7 in early porcine embryonic development remains unclear. RESULTS: In this study, we found that ATF7 gradually accumulated in the nucleus and then localized on the pericentric heterochromatin after the late 4‐cell stage, while being co‐localized with heterochromatin protein 1 (HP1). Knockdown of ATF7 resulted in decreases in the blastocyst rate and blastocyst cell number. ATF7 depletion resulted in downregulation of HP1 and histone 3 lysine 9 dimethylation (H3K9me2) expression. These effects were alleviated when P38 activity was inhibited. High temperatures increased the expression level of pP38, while reducing the quality of porcine embryos, and led to ATF7 phosphorylation. The expression level of H3K9me2 and HP1 was decreased and regulated by P38 activity. CONCLUSION: Stress‐induced ATF7‐dependent epigenetic changes play important roles in early porcine embryonic development. John Wiley and Sons Inc. 2022-10-18 /pmc/articles/PMC9890523/ /pubmed/36254813 http://dx.doi.org/10.1111/cpr.13352 Text en © 2022 The Authors. Cell Proliferation published by Beijing Institute for Stem Cell and Regenerative Medicine and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Sun, Ming‐Hong Jiang, Wen‐Jie Li, Xiao‐Han Lee, Song‐Hee Heo, Geun Zhou, Dongjie Choi, Jung‐Seok Kim, Kwan‐Suk Lv, Wenfa Cui, Xiang‐Shun ATF7‐dependent epigenetic changes induced by high temperature during early porcine embryonic development |
title |
ATF7‐dependent epigenetic changes induced by high temperature during early porcine embryonic development |
title_full |
ATF7‐dependent epigenetic changes induced by high temperature during early porcine embryonic development |
title_fullStr |
ATF7‐dependent epigenetic changes induced by high temperature during early porcine embryonic development |
title_full_unstemmed |
ATF7‐dependent epigenetic changes induced by high temperature during early porcine embryonic development |
title_short |
ATF7‐dependent epigenetic changes induced by high temperature during early porcine embryonic development |
title_sort | atf7‐dependent epigenetic changes induced by high temperature during early porcine embryonic development |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9890523/ https://www.ncbi.nlm.nih.gov/pubmed/36254813 http://dx.doi.org/10.1111/cpr.13352 |
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