Towards the use of an amino acid cleavable linker for solid-phase chemical synthesis of peptides and proteins

The synthesis of proteins by solid-phase chemical ligation (SPCL) suffers from the paucity of linkers that can be cleaved under mild conditions. Here, we deployed a spontaneous nickel-assisted cleavage (SNAC) tag, known to undergo spontaneous cleavage in the presence of nickel(ii), as a linker for C...

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Detalles Bibliográficos
Autores principales: Cardella, Davide, Tsai, Yu-Hsuan, Luk, Louis Y. P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2022
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9890637/
https://www.ncbi.nlm.nih.gov/pubmed/36628630
http://dx.doi.org/10.1039/d2ob02198f
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author Cardella, Davide
Tsai, Yu-Hsuan
Luk, Louis Y. P.
author_facet Cardella, Davide
Tsai, Yu-Hsuan
Luk, Louis Y. P.
author_sort Cardella, Davide
collection PubMed
description The synthesis of proteins by solid-phase chemical ligation (SPCL) suffers from the paucity of linkers that can be cleaved under mild conditions. Here, we deployed a spontaneous nickel-assisted cleavage (SNAC) tag, known to undergo spontaneous cleavage in the presence of nickel(ii), as a linker for C-to-N SPCL.
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spelling pubmed-98906372023-02-02 Towards the use of an amino acid cleavable linker for solid-phase chemical synthesis of peptides and proteins Cardella, Davide Tsai, Yu-Hsuan Luk, Louis Y. P. Org Biomol Chem Chemistry The synthesis of proteins by solid-phase chemical ligation (SPCL) suffers from the paucity of linkers that can be cleaved under mild conditions. Here, we deployed a spontaneous nickel-assisted cleavage (SNAC) tag, known to undergo spontaneous cleavage in the presence of nickel(ii), as a linker for C-to-N SPCL. The Royal Society of Chemistry 2022-12-23 /pmc/articles/PMC9890637/ /pubmed/36628630 http://dx.doi.org/10.1039/d2ob02198f Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/
spellingShingle Chemistry
Cardella, Davide
Tsai, Yu-Hsuan
Luk, Louis Y. P.
Towards the use of an amino acid cleavable linker for solid-phase chemical synthesis of peptides and proteins
title Towards the use of an amino acid cleavable linker for solid-phase chemical synthesis of peptides and proteins
title_full Towards the use of an amino acid cleavable linker for solid-phase chemical synthesis of peptides and proteins
title_fullStr Towards the use of an amino acid cleavable linker for solid-phase chemical synthesis of peptides and proteins
title_full_unstemmed Towards the use of an amino acid cleavable linker for solid-phase chemical synthesis of peptides and proteins
title_short Towards the use of an amino acid cleavable linker for solid-phase chemical synthesis of peptides and proteins
title_sort towards the use of an amino acid cleavable linker for solid-phase chemical synthesis of peptides and proteins
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9890637/
https://www.ncbi.nlm.nih.gov/pubmed/36628630
http://dx.doi.org/10.1039/d2ob02198f
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