Barriers to simultaneous multilocus integration in Bacillus subtilis tumble down: development of a straightforward screening method for the colorimetric detection of one-step multiple gene insertion using the CRISPR-Cas9 system

BACKGROUND: Despite recent advances in genetic engineering tools for effectively regulating and manipulating genes, efficient simultaneous multigene insertion methods have not been established in Bacillus subtilis. To date, multilocus integration systems in B. subtilis, which is one of the main indu...

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Detalles Bibliográficos
Autores principales: Ferrando, Jordi, Filluelo, Oriana, Zeigler, Daniel R., Picart, Pere
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9890709/
https://www.ncbi.nlm.nih.gov/pubmed/36721198
http://dx.doi.org/10.1186/s12934-023-02032-2

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