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Nr4a1-dependent non-classical monocytes are important for macrophage-mediated wound healing in the large intestine
INTRODUCTION: Macrophages play an important role in intestinal wound healing. However, the trajectories from circulating monocytes to gut macrophages are incompletely understood. METHODS: Taking advantage of mice depleted for non-classical monocytes due to deficiency for the transcription factor Nr4...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9890957/ https://www.ncbi.nlm.nih.gov/pubmed/36741412 http://dx.doi.org/10.3389/fimmu.2022.1040775 |
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author | Heidbreder, Karin Sommer, Katrin Wiendl, Maximilian Müller, Tanja M. Atreya, Imke Hildner, Kai Neurath, Markus F. Zundler, Sebastian |
author_facet | Heidbreder, Karin Sommer, Katrin Wiendl, Maximilian Müller, Tanja M. Atreya, Imke Hildner, Kai Neurath, Markus F. Zundler, Sebastian |
author_sort | Heidbreder, Karin |
collection | PubMed |
description | INTRODUCTION: Macrophages play an important role in intestinal wound healing. However, the trajectories from circulating monocytes to gut macrophages are incompletely understood. METHODS: Taking advantage of mice depleted for non-classical monocytes due to deficiency for the transcription factor Nr4a1, we addressed the relevance of non-classical monocytes for large intestinal wound healing using flow cytometry, in vivo wound healing assays and immunofluorescence. RESULTS: We show that wound healing in Nr4a1-deficient mice is substantially delayed and associated with reduced peri-lesional presence of macrophages with a wound healing phenotype. DISCUSSION: Our data suggest that non-classical monocytes are biased towards wound healing macrophages. These insights might help to understand, how targeting monocyte recruitment to the intestine can be used to modulate intestinal macrophage functions. |
format | Online Article Text |
id | pubmed-9890957 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-98909572023-02-02 Nr4a1-dependent non-classical monocytes are important for macrophage-mediated wound healing in the large intestine Heidbreder, Karin Sommer, Katrin Wiendl, Maximilian Müller, Tanja M. Atreya, Imke Hildner, Kai Neurath, Markus F. Zundler, Sebastian Front Immunol Immunology INTRODUCTION: Macrophages play an important role in intestinal wound healing. However, the trajectories from circulating monocytes to gut macrophages are incompletely understood. METHODS: Taking advantage of mice depleted for non-classical monocytes due to deficiency for the transcription factor Nr4a1, we addressed the relevance of non-classical monocytes for large intestinal wound healing using flow cytometry, in vivo wound healing assays and immunofluorescence. RESULTS: We show that wound healing in Nr4a1-deficient mice is substantially delayed and associated with reduced peri-lesional presence of macrophages with a wound healing phenotype. DISCUSSION: Our data suggest that non-classical monocytes are biased towards wound healing macrophages. These insights might help to understand, how targeting monocyte recruitment to the intestine can be used to modulate intestinal macrophage functions. Frontiers Media S.A. 2023-01-18 /pmc/articles/PMC9890957/ /pubmed/36741412 http://dx.doi.org/10.3389/fimmu.2022.1040775 Text en Copyright © 2023 Heidbreder, Sommer, Wiendl, Müller, Atreya, Hildner, Neurath and Zundler https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology Heidbreder, Karin Sommer, Katrin Wiendl, Maximilian Müller, Tanja M. Atreya, Imke Hildner, Kai Neurath, Markus F. Zundler, Sebastian Nr4a1-dependent non-classical monocytes are important for macrophage-mediated wound healing in the large intestine |
title | Nr4a1-dependent non-classical monocytes are important for macrophage-mediated wound healing in the large intestine |
title_full | Nr4a1-dependent non-classical monocytes are important for macrophage-mediated wound healing in the large intestine |
title_fullStr | Nr4a1-dependent non-classical monocytes are important for macrophage-mediated wound healing in the large intestine |
title_full_unstemmed | Nr4a1-dependent non-classical monocytes are important for macrophage-mediated wound healing in the large intestine |
title_short | Nr4a1-dependent non-classical monocytes are important for macrophage-mediated wound healing in the large intestine |
title_sort | nr4a1-dependent non-classical monocytes are important for macrophage-mediated wound healing in the large intestine |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9890957/ https://www.ncbi.nlm.nih.gov/pubmed/36741412 http://dx.doi.org/10.3389/fimmu.2022.1040775 |
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