A Carbon Paste Electrode Modified by Bentonite and l‐Cysteine for Simultaneous Determination of Ascorbic and Uric Acids: Application in Biological Fluids

A novel modification of a paste carbon electrode by Bentonite (Bent) and l‐Cysteine (l‐Cyst) was carried out for uric acid (UA) and ascorbic acid (AA) detection and quantification. Morphological and compositional characterization of the electrode surface were carried out using electrochemical impeda...

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Autores principales: Choukairi, Mohamed, Bouchta, Dounia, Bounab, Loubna, González‐Romero, Elisa, Achache, Mohamed, Draoui, Khalid, Chaouket, Faiza, Raissouni, Ihssane, Gharous, Mouad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2023
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Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9891123/
https://www.ncbi.nlm.nih.gov/pubmed/36722827
http://dx.doi.org/10.1002/open.202200201
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author Choukairi, Mohamed
Bouchta, Dounia
Bounab, Loubna
González‐Romero, Elisa
Achache, Mohamed
Draoui, Khalid
Chaouket, Faiza
Raissouni, Ihssane
Gharous, Mouad
author_facet Choukairi, Mohamed
Bouchta, Dounia
Bounab, Loubna
González‐Romero, Elisa
Achache, Mohamed
Draoui, Khalid
Chaouket, Faiza
Raissouni, Ihssane
Gharous, Mouad
author_sort Choukairi, Mohamed
collection PubMed
description A novel modification of a paste carbon electrode by Bentonite (Bent) and l‐Cysteine (l‐Cyst) was carried out for uric acid (UA) and ascorbic acid (AA) detection and quantification. Morphological and compositional characterization of the electrode surface were carried out using electrochemical impedance spectroscopy (EIS), scanning electron microscopy (SEM) and energy dispersive X‐ray spectroscopic analysis (EDS). Cyclic voltammetry (CV) and square wave voltammetry (SWV) techniques were used to analyze UA and AA. The obtained sensor shows a good stability, sensibility, selectivity, and regeneration ability. Accordingly, the limit of detection (LOD) is found to be 0.031 μm and 9.6 μm for UA and AA, respectively. A good linearity in the range of 0.1 to 100 μm for UA and 10 to 1000 μm for AA was obtained. The peak‐to‐peak separation of UA‐AA (ΔE (UA‐AA)) was determined to be 330 mV. In addition, the sensor is applied successfully to monitor UA and AA in serum samples.
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spelling pubmed-98911232023-02-02 A Carbon Paste Electrode Modified by Bentonite and l‐Cysteine for Simultaneous Determination of Ascorbic and Uric Acids: Application in Biological Fluids Choukairi, Mohamed Bouchta, Dounia Bounab, Loubna González‐Romero, Elisa Achache, Mohamed Draoui, Khalid Chaouket, Faiza Raissouni, Ihssane Gharous, Mouad ChemistryOpen Research Articles A novel modification of a paste carbon electrode by Bentonite (Bent) and l‐Cysteine (l‐Cyst) was carried out for uric acid (UA) and ascorbic acid (AA) detection and quantification. Morphological and compositional characterization of the electrode surface were carried out using electrochemical impedance spectroscopy (EIS), scanning electron microscopy (SEM) and energy dispersive X‐ray spectroscopic analysis (EDS). Cyclic voltammetry (CV) and square wave voltammetry (SWV) techniques were used to analyze UA and AA. The obtained sensor shows a good stability, sensibility, selectivity, and regeneration ability. Accordingly, the limit of detection (LOD) is found to be 0.031 μm and 9.6 μm for UA and AA, respectively. A good linearity in the range of 0.1 to 100 μm for UA and 10 to 1000 μm for AA was obtained. The peak‐to‐peak separation of UA‐AA (ΔE (UA‐AA)) was determined to be 330 mV. In addition, the sensor is applied successfully to monitor UA and AA in serum samples. John Wiley and Sons Inc. 2023-02-01 /pmc/articles/PMC9891123/ /pubmed/36722827 http://dx.doi.org/10.1002/open.202200201 Text en © 2023 The Authors. Published by Wiley-VCH GmbH https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Research Articles
Choukairi, Mohamed
Bouchta, Dounia
Bounab, Loubna
González‐Romero, Elisa
Achache, Mohamed
Draoui, Khalid
Chaouket, Faiza
Raissouni, Ihssane
Gharous, Mouad
A Carbon Paste Electrode Modified by Bentonite and l‐Cysteine for Simultaneous Determination of Ascorbic and Uric Acids: Application in Biological Fluids
title A Carbon Paste Electrode Modified by Bentonite and l‐Cysteine for Simultaneous Determination of Ascorbic and Uric Acids: Application in Biological Fluids
title_full A Carbon Paste Electrode Modified by Bentonite and l‐Cysteine for Simultaneous Determination of Ascorbic and Uric Acids: Application in Biological Fluids
title_fullStr A Carbon Paste Electrode Modified by Bentonite and l‐Cysteine for Simultaneous Determination of Ascorbic and Uric Acids: Application in Biological Fluids
title_full_unstemmed A Carbon Paste Electrode Modified by Bentonite and l‐Cysteine for Simultaneous Determination of Ascorbic and Uric Acids: Application in Biological Fluids
title_short A Carbon Paste Electrode Modified by Bentonite and l‐Cysteine for Simultaneous Determination of Ascorbic and Uric Acids: Application in Biological Fluids
title_sort carbon paste electrode modified by bentonite and l‐cysteine for simultaneous determination of ascorbic and uric acids: application in biological fluids
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9891123/
https://www.ncbi.nlm.nih.gov/pubmed/36722827
http://dx.doi.org/10.1002/open.202200201
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