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Safety and immunogenicity of a combined DTacP-sIPV-Hib vaccine in animal models

The DTacP-sIPV-Hib combination vaccine can replace the single-component acellular pertussis, diphtheria, tetanus, polio, and Haemophilus influenzae type B vaccines. In this study, we evaluated the safety and immunogenicity of a newly developed DTacP-sIPV-Hib combination vaccine in animal models. We...

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Autores principales: Zhang, Yuntao, Guo, Yancen, Dong, Yuan, Liu, Yingwei, Zhao, Yuxiu, Yu, Shouzhi, Li, Shihui, Wu, Chongyang, Yang, Baifeng, Li, Wanli, Wei, Xiaoli, Zhang, Yadan, Huang, Yunchao, Wang, Hui, Yang, Xiaoming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9891680/
https://www.ncbi.nlm.nih.gov/pubmed/36576263
http://dx.doi.org/10.1080/21645515.2022.2160158
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author Zhang, Yuntao
Guo, Yancen
Dong, Yuan
Liu, Yingwei
Zhao, Yuxiu
Yu, Shouzhi
Li, Shihui
Wu, Chongyang
Yang, Baifeng
Li, Wanli
Wei, Xiaoli
Zhang, Yadan
Huang, Yunchao
Wang, Hui
Yang, Xiaoming
author_facet Zhang, Yuntao
Guo, Yancen
Dong, Yuan
Liu, Yingwei
Zhao, Yuxiu
Yu, Shouzhi
Li, Shihui
Wu, Chongyang
Yang, Baifeng
Li, Wanli
Wei, Xiaoli
Zhang, Yadan
Huang, Yunchao
Wang, Hui
Yang, Xiaoming
author_sort Zhang, Yuntao
collection PubMed
description The DTacP-sIPV-Hib combination vaccine can replace the single-component acellular pertussis, diphtheria, tetanus, polio, and Haemophilus influenzae type B vaccines. In this study, we evaluated the safety and immunogenicity of a newly developed DTacP-sIPV-Hib combination vaccine in animal models. We used 40 mice and 46 cynomolgus monkeys to evaluate acute and long-term toxicity. Thirty-six guinea pigs were used for sensitization assessment. For immunogenicity assessment, 50 NIH mice and 50 rats were equally randomized to receive 3 doses of 3 different batches of the tested vaccine at an interval of 21 d, or physiological saline solution (0.5 mL). Orbital blood was collected at an interval of 21 d post inoculation to detect related antibody titers or neutralizing antibody titers against poliovirus. Gross autopsy and histopathological examination revealed no abnormal toxicity or irritation in mice and cynomolgus monkeys. Sensitization assessment in guinea pigs indicated the lack of evident allergic symptoms in the high- and low-dose vaccine groups within 30 min after repeated stimulation. The DTacP-sIPV-Hib combination vaccine induced significant immune responses in mice, rats, and cynomolgus monkeys, with 100% seroconversion rates after 3 doses. The DTacP-sIPV-Hib combination vaccine is safe and immunogenic in animal models. Three doses of the vaccine elicited satisfactory antibody responses in mice, rats, and cynomolgus monkeys.
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spelling pubmed-98916802023-02-02 Safety and immunogenicity of a combined DTacP-sIPV-Hib vaccine in animal models Zhang, Yuntao Guo, Yancen Dong, Yuan Liu, Yingwei Zhao, Yuxiu Yu, Shouzhi Li, Shihui Wu, Chongyang Yang, Baifeng Li, Wanli Wei, Xiaoli Zhang, Yadan Huang, Yunchao Wang, Hui Yang, Xiaoming Hum Vaccin Immunother Licensed Vaccines – Research Article The DTacP-sIPV-Hib combination vaccine can replace the single-component acellular pertussis, diphtheria, tetanus, polio, and Haemophilus influenzae type B vaccines. In this study, we evaluated the safety and immunogenicity of a newly developed DTacP-sIPV-Hib combination vaccine in animal models. We used 40 mice and 46 cynomolgus monkeys to evaluate acute and long-term toxicity. Thirty-six guinea pigs were used for sensitization assessment. For immunogenicity assessment, 50 NIH mice and 50 rats were equally randomized to receive 3 doses of 3 different batches of the tested vaccine at an interval of 21 d, or physiological saline solution (0.5 mL). Orbital blood was collected at an interval of 21 d post inoculation to detect related antibody titers or neutralizing antibody titers against poliovirus. Gross autopsy and histopathological examination revealed no abnormal toxicity or irritation in mice and cynomolgus monkeys. Sensitization assessment in guinea pigs indicated the lack of evident allergic symptoms in the high- and low-dose vaccine groups within 30 min after repeated stimulation. The DTacP-sIPV-Hib combination vaccine induced significant immune responses in mice, rats, and cynomolgus monkeys, with 100% seroconversion rates after 3 doses. The DTacP-sIPV-Hib combination vaccine is safe and immunogenic in animal models. Three doses of the vaccine elicited satisfactory antibody responses in mice, rats, and cynomolgus monkeys. Taylor & Francis 2022-12-28 /pmc/articles/PMC9891680/ /pubmed/36576263 http://dx.doi.org/10.1080/21645515.2022.2160158 Text en © 2022 The Author(s). Published with license by Taylor & Francis Group, LLC. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.
spellingShingle Licensed Vaccines – Research Article
Zhang, Yuntao
Guo, Yancen
Dong, Yuan
Liu, Yingwei
Zhao, Yuxiu
Yu, Shouzhi
Li, Shihui
Wu, Chongyang
Yang, Baifeng
Li, Wanli
Wei, Xiaoli
Zhang, Yadan
Huang, Yunchao
Wang, Hui
Yang, Xiaoming
Safety and immunogenicity of a combined DTacP-sIPV-Hib vaccine in animal models
title Safety and immunogenicity of a combined DTacP-sIPV-Hib vaccine in animal models
title_full Safety and immunogenicity of a combined DTacP-sIPV-Hib vaccine in animal models
title_fullStr Safety and immunogenicity of a combined DTacP-sIPV-Hib vaccine in animal models
title_full_unstemmed Safety and immunogenicity of a combined DTacP-sIPV-Hib vaccine in animal models
title_short Safety and immunogenicity of a combined DTacP-sIPV-Hib vaccine in animal models
title_sort safety and immunogenicity of a combined dtacp-sipv-hib vaccine in animal models
topic Licensed Vaccines – Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9891680/
https://www.ncbi.nlm.nih.gov/pubmed/36576263
http://dx.doi.org/10.1080/21645515.2022.2160158
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