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Effects of medwakh smoking on salivary metabolomics and its association with altered oral redox homeostasis among youth

The use of alternative tobacco products, particularly medwakh, has expanded among youth in the Middle East and around the world. The present study is conducted to investigate the biochemical and pathophysiological changes caused by medwakh smoking, and to examine the salivary metabolomics profile of...

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Autores principales: Aghila Rani, K. G., Soares, Nelson C., Rahman, Betul, Al-Hroub, Hamza M., Semreen, Mohammad H., Al Kawas, Sausan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9891755/
https://www.ncbi.nlm.nih.gov/pubmed/36725974
http://dx.doi.org/10.1038/s41598-023-27958-w
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author Aghila Rani, K. G.
Soares, Nelson C.
Rahman, Betul
Al-Hroub, Hamza M.
Semreen, Mohammad H.
Al Kawas, Sausan
author_facet Aghila Rani, K. G.
Soares, Nelson C.
Rahman, Betul
Al-Hroub, Hamza M.
Semreen, Mohammad H.
Al Kawas, Sausan
author_sort Aghila Rani, K. G.
collection PubMed
description The use of alternative tobacco products, particularly medwakh, has expanded among youth in the Middle East and around the world. The present study is conducted to investigate the biochemical and pathophysiological changes caused by medwakh smoking, and to examine the salivary metabolomics profile of medwakh smokers. Saliva samples were collected from 30 non-smokers and 30 medwakh smokers and subjected to metabolomic analysis by UHPLC-ESI-QTOF-MS. The CRP and Glutathione Peroxidase 1 activity levels in the study samples were quantified by ELISA and the total antioxidant capacity (TAC) by TAC assay kits. Statistical measurements and thorough validation of data obtained from untargeted metabolomics identified 37 uniquely and differentially abundant metabolites in saliva of medwakh smokers. The levels of phthalate, L-sorbose, cytosine, uridine, alpha-hydroxy hippurate, and L-nicotine were noticeably high in medwakh smokers. Likewise, 20 metabolic pathways were differentially altered in medwakh smokers. This study identified a distinctive saliva metabolomics profile in medwakh smokers associated with altered redox homeostasis, metabolic pathways, antioxidant system, and CRP levels. The impact of the altered metabolites in medwakh smokers and their diagnostic utility require further research in large cohorts.
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spelling pubmed-98917552023-02-02 Effects of medwakh smoking on salivary metabolomics and its association with altered oral redox homeostasis among youth Aghila Rani, K. G. Soares, Nelson C. Rahman, Betul Al-Hroub, Hamza M. Semreen, Mohammad H. Al Kawas, Sausan Sci Rep Article The use of alternative tobacco products, particularly medwakh, has expanded among youth in the Middle East and around the world. The present study is conducted to investigate the biochemical and pathophysiological changes caused by medwakh smoking, and to examine the salivary metabolomics profile of medwakh smokers. Saliva samples were collected from 30 non-smokers and 30 medwakh smokers and subjected to metabolomic analysis by UHPLC-ESI-QTOF-MS. The CRP and Glutathione Peroxidase 1 activity levels in the study samples were quantified by ELISA and the total antioxidant capacity (TAC) by TAC assay kits. Statistical measurements and thorough validation of data obtained from untargeted metabolomics identified 37 uniquely and differentially abundant metabolites in saliva of medwakh smokers. The levels of phthalate, L-sorbose, cytosine, uridine, alpha-hydroxy hippurate, and L-nicotine were noticeably high in medwakh smokers. Likewise, 20 metabolic pathways were differentially altered in medwakh smokers. This study identified a distinctive saliva metabolomics profile in medwakh smokers associated with altered redox homeostasis, metabolic pathways, antioxidant system, and CRP levels. The impact of the altered metabolites in medwakh smokers and their diagnostic utility require further research in large cohorts. Nature Publishing Group UK 2023-02-01 /pmc/articles/PMC9891755/ /pubmed/36725974 http://dx.doi.org/10.1038/s41598-023-27958-w Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Aghila Rani, K. G.
Soares, Nelson C.
Rahman, Betul
Al-Hroub, Hamza M.
Semreen, Mohammad H.
Al Kawas, Sausan
Effects of medwakh smoking on salivary metabolomics and its association with altered oral redox homeostasis among youth
title Effects of medwakh smoking on salivary metabolomics and its association with altered oral redox homeostasis among youth
title_full Effects of medwakh smoking on salivary metabolomics and its association with altered oral redox homeostasis among youth
title_fullStr Effects of medwakh smoking on salivary metabolomics and its association with altered oral redox homeostasis among youth
title_full_unstemmed Effects of medwakh smoking on salivary metabolomics and its association with altered oral redox homeostasis among youth
title_short Effects of medwakh smoking on salivary metabolomics and its association with altered oral redox homeostasis among youth
title_sort effects of medwakh smoking on salivary metabolomics and its association with altered oral redox homeostasis among youth
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9891755/
https://www.ncbi.nlm.nih.gov/pubmed/36725974
http://dx.doi.org/10.1038/s41598-023-27958-w
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