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Immunogenicity Evaluation of Thermostable Microparticles Entrapping Receptor Binding Domain of SARS-CoV-2 by Single Point Administration

Receptor binding domain (RBD) of SARS-CoV-2 is a prime vaccine target against which neutralizing antibody responses are directed. Purified RBD as a vaccine candidate warrants administration of multiple doses along with adjuvants and use of delivery systems to improve its immunogenicity. The present...

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Autores principales: Ahuja, Rahul, Srichandan, Sudeepa, Meena, Jairam, Biswal, Bichitra Kumar, Panda, Amulya K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Pharmacists Association. Published by Elsevier Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9891792/
https://www.ncbi.nlm.nih.gov/pubmed/36736778
http://dx.doi.org/10.1016/j.xphs.2023.01.024
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author Ahuja, Rahul
Srichandan, Sudeepa
Meena, Jairam
Biswal, Bichitra Kumar
Panda, Amulya K.
author_facet Ahuja, Rahul
Srichandan, Sudeepa
Meena, Jairam
Biswal, Bichitra Kumar
Panda, Amulya K.
author_sort Ahuja, Rahul
collection PubMed
description Receptor binding domain (RBD) of SARS-CoV-2 is a prime vaccine target against which neutralizing antibody responses are directed. Purified RBD as a vaccine candidate warrants administration of multiple doses along with adjuvants and use of delivery systems to improve its immunogenicity. The present investigation examines the immunogenicity of RBD delivered by biodegradable polymer particles from single dose administration. Mice upon single point immunization of RBD entrapped microparticles generated improved antibody response. The polymer microparticles showed better temperature stability and could be stored at 37 degrees for one month without any considerable loss of immunogenicity. Further, immunization with microparticles could elicit memory antibody response upon challenge after four months of single dose administration. Thus, using microparticles entrapping RBD as a vaccine candidate confer improved immunogenicity, temperature stability and recall response. These thermostable microparticles seem to be a potentially cost-effective approach which can help in dose reduction, provide a wider access of vaccines and accelerate the end of global pandemic.
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spelling pubmed-98917922023-02-02 Immunogenicity Evaluation of Thermostable Microparticles Entrapping Receptor Binding Domain of SARS-CoV-2 by Single Point Administration Ahuja, Rahul Srichandan, Sudeepa Meena, Jairam Biswal, Bichitra Kumar Panda, Amulya K. J Pharm Sci Pharmaceutics, Drug Delivery and Pharmaceutical Technology Receptor binding domain (RBD) of SARS-CoV-2 is a prime vaccine target against which neutralizing antibody responses are directed. Purified RBD as a vaccine candidate warrants administration of multiple doses along with adjuvants and use of delivery systems to improve its immunogenicity. The present investigation examines the immunogenicity of RBD delivered by biodegradable polymer particles from single dose administration. Mice upon single point immunization of RBD entrapped microparticles generated improved antibody response. The polymer microparticles showed better temperature stability and could be stored at 37 degrees for one month without any considerable loss of immunogenicity. Further, immunization with microparticles could elicit memory antibody response upon challenge after four months of single dose administration. Thus, using microparticles entrapping RBD as a vaccine candidate confer improved immunogenicity, temperature stability and recall response. These thermostable microparticles seem to be a potentially cost-effective approach which can help in dose reduction, provide a wider access of vaccines and accelerate the end of global pandemic. American Pharmacists Association. Published by Elsevier Inc. 2023-06 2023-02-02 /pmc/articles/PMC9891792/ /pubmed/36736778 http://dx.doi.org/10.1016/j.xphs.2023.01.024 Text en © 2023 American Pharmacists Association. Published by Elsevier Inc. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Pharmaceutics, Drug Delivery and Pharmaceutical Technology
Ahuja, Rahul
Srichandan, Sudeepa
Meena, Jairam
Biswal, Bichitra Kumar
Panda, Amulya K.
Immunogenicity Evaluation of Thermostable Microparticles Entrapping Receptor Binding Domain of SARS-CoV-2 by Single Point Administration
title Immunogenicity Evaluation of Thermostable Microparticles Entrapping Receptor Binding Domain of SARS-CoV-2 by Single Point Administration
title_full Immunogenicity Evaluation of Thermostable Microparticles Entrapping Receptor Binding Domain of SARS-CoV-2 by Single Point Administration
title_fullStr Immunogenicity Evaluation of Thermostable Microparticles Entrapping Receptor Binding Domain of SARS-CoV-2 by Single Point Administration
title_full_unstemmed Immunogenicity Evaluation of Thermostable Microparticles Entrapping Receptor Binding Domain of SARS-CoV-2 by Single Point Administration
title_short Immunogenicity Evaluation of Thermostable Microparticles Entrapping Receptor Binding Domain of SARS-CoV-2 by Single Point Administration
title_sort immunogenicity evaluation of thermostable microparticles entrapping receptor binding domain of sars-cov-2 by single point administration
topic Pharmaceutics, Drug Delivery and Pharmaceutical Technology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9891792/
https://www.ncbi.nlm.nih.gov/pubmed/36736778
http://dx.doi.org/10.1016/j.xphs.2023.01.024
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