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Healing of the Torn Anterior Horn of Rabbit Medial Meniscus to Bone after Transtibial Pull‐Out Repair and Autologous Platelet‐Rich Plasma Gel Injection

OBJECTIVES: The transtibial pull‐out repair (TP) is a relatively new method for treating meniscal root tear; however, the clinical evaluation of its healing effect remains controversial. Due to ethical constraints and limitations of imaging techniques in humans, here we dynamically observe the heali...

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Autores principales: Cui, Peng, Sun, Bai‐hai, Dai, Ya‐feng, Cui, Tian‐yi, Sun, Jing‐lei, Shen, Ke, Zhang, Lian‐shan, Shi, Chen‐xia, Wang, Xiao‐feng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons Australia, Ltd 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9891914/
https://www.ncbi.nlm.nih.gov/pubmed/36573287
http://dx.doi.org/10.1111/os.13622
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author Cui, Peng
Sun, Bai‐hai
Dai, Ya‐feng
Cui, Tian‐yi
Sun, Jing‐lei
Shen, Ke
Zhang, Lian‐shan
Shi, Chen‐xia
Wang, Xiao‐feng
author_facet Cui, Peng
Sun, Bai‐hai
Dai, Ya‐feng
Cui, Tian‐yi
Sun, Jing‐lei
Shen, Ke
Zhang, Lian‐shan
Shi, Chen‐xia
Wang, Xiao‐feng
author_sort Cui, Peng
collection PubMed
description OBJECTIVES: The transtibial pull‐out repair (TP) is a relatively new method for treating meniscal root tear; however, the clinical evaluation of its healing effect remains controversial. Due to ethical constraints and limitations of imaging techniques in humans, here we dynamically observe the healing effects of TP and TP with platelet‐rich plasma gel (PRG) at the histological level using an animal model. METHODS: Platelet‐rich plasma (PRP) and PRG of rabbits were prepared. Platelet‐derived growth factor (PDGF) and transforming growth factor‐β1 (TGF‐β1) levels in PRP and PRG were determined using an enzyme‐linked immunosorbent assay. A rabbit model of anterior horn tear of the medial meniscus and TP surgery were created. PRG was injected between the anterior horn of the medial meniscus and the tibial tunnel. Rabbits were divided into three groups: the anterior horn tear group (Tear group), the anterior horn tear + TP group (TP group), and the anterior horn tear + TP + PRG group (TP + PRG group). The healing effect was observed dynamically using histopathological studies and biomechanical experiments. RESULTS: The platelet content in PRP significantly increased to approximately 4.57 times that of whole blood. PDGF and TGF‐β1 concentrations in PRG increased to 2.46 and 4.15 times those in PRP, respectively. Hematoxylin and eosin (H&E) and Masson staining showed that the number of inflammatory cells in healing tissue decreased and the collagen fibers significantly increased in TP and TP + PRG groups at 4, 8, and 12 weeks postoperatively compared to those in Tear group. Neatly arranged, interlaced, and dense collagen fibers were found between the anterior horn and bone at 12 weeks. H&E and toluidine blue staining showed that the injury to the femoral condyle cartilage was alleviated. The healing performance in TP + PRG group was better and faster than that in TP group. The maximum tensile fracture strength of the meniscus progressively increased at 8 and 12 weeks postoperatively. CONCLUSIONS: Anterior horn injury of the medial meniscus in rabbits can be repaired using the TP technique, and the addition of autologous PRG to the bone tunnel promotes early healing of the meniscus and bone postoperatively. Meanwhile, both treatments can reduce the secondary damage to the cartilage due to osteoarthritis.
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spelling pubmed-98919142023-02-02 Healing of the Torn Anterior Horn of Rabbit Medial Meniscus to Bone after Transtibial Pull‐Out Repair and Autologous Platelet‐Rich Plasma Gel Injection Cui, Peng Sun, Bai‐hai Dai, Ya‐feng Cui, Tian‐yi Sun, Jing‐lei Shen, Ke Zhang, Lian‐shan Shi, Chen‐xia Wang, Xiao‐feng Orthop Surg Research Articles OBJECTIVES: The transtibial pull‐out repair (TP) is a relatively new method for treating meniscal root tear; however, the clinical evaluation of its healing effect remains controversial. Due to ethical constraints and limitations of imaging techniques in humans, here we dynamically observe the healing effects of TP and TP with platelet‐rich plasma gel (PRG) at the histological level using an animal model. METHODS: Platelet‐rich plasma (PRP) and PRG of rabbits were prepared. Platelet‐derived growth factor (PDGF) and transforming growth factor‐β1 (TGF‐β1) levels in PRP and PRG were determined using an enzyme‐linked immunosorbent assay. A rabbit model of anterior horn tear of the medial meniscus and TP surgery were created. PRG was injected between the anterior horn of the medial meniscus and the tibial tunnel. Rabbits were divided into three groups: the anterior horn tear group (Tear group), the anterior horn tear + TP group (TP group), and the anterior horn tear + TP + PRG group (TP + PRG group). The healing effect was observed dynamically using histopathological studies and biomechanical experiments. RESULTS: The platelet content in PRP significantly increased to approximately 4.57 times that of whole blood. PDGF and TGF‐β1 concentrations in PRG increased to 2.46 and 4.15 times those in PRP, respectively. Hematoxylin and eosin (H&E) and Masson staining showed that the number of inflammatory cells in healing tissue decreased and the collagen fibers significantly increased in TP and TP + PRG groups at 4, 8, and 12 weeks postoperatively compared to those in Tear group. Neatly arranged, interlaced, and dense collagen fibers were found between the anterior horn and bone at 12 weeks. H&E and toluidine blue staining showed that the injury to the femoral condyle cartilage was alleviated. The healing performance in TP + PRG group was better and faster than that in TP group. The maximum tensile fracture strength of the meniscus progressively increased at 8 and 12 weeks postoperatively. CONCLUSIONS: Anterior horn injury of the medial meniscus in rabbits can be repaired using the TP technique, and the addition of autologous PRG to the bone tunnel promotes early healing of the meniscus and bone postoperatively. Meanwhile, both treatments can reduce the secondary damage to the cartilage due to osteoarthritis. John Wiley & Sons Australia, Ltd 2022-12-26 /pmc/articles/PMC9891914/ /pubmed/36573287 http://dx.doi.org/10.1111/os.13622 Text en © 2022 The Authors. Orthopaedic Surgery published by Tianjin Hospital and John Wiley & Sons Australia, Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Research Articles
Cui, Peng
Sun, Bai‐hai
Dai, Ya‐feng
Cui, Tian‐yi
Sun, Jing‐lei
Shen, Ke
Zhang, Lian‐shan
Shi, Chen‐xia
Wang, Xiao‐feng
Healing of the Torn Anterior Horn of Rabbit Medial Meniscus to Bone after Transtibial Pull‐Out Repair and Autologous Platelet‐Rich Plasma Gel Injection
title Healing of the Torn Anterior Horn of Rabbit Medial Meniscus to Bone after Transtibial Pull‐Out Repair and Autologous Platelet‐Rich Plasma Gel Injection
title_full Healing of the Torn Anterior Horn of Rabbit Medial Meniscus to Bone after Transtibial Pull‐Out Repair and Autologous Platelet‐Rich Plasma Gel Injection
title_fullStr Healing of the Torn Anterior Horn of Rabbit Medial Meniscus to Bone after Transtibial Pull‐Out Repair and Autologous Platelet‐Rich Plasma Gel Injection
title_full_unstemmed Healing of the Torn Anterior Horn of Rabbit Medial Meniscus to Bone after Transtibial Pull‐Out Repair and Autologous Platelet‐Rich Plasma Gel Injection
title_short Healing of the Torn Anterior Horn of Rabbit Medial Meniscus to Bone after Transtibial Pull‐Out Repair and Autologous Platelet‐Rich Plasma Gel Injection
title_sort healing of the torn anterior horn of rabbit medial meniscus to bone after transtibial pull‐out repair and autologous platelet‐rich plasma gel injection
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9891914/
https://www.ncbi.nlm.nih.gov/pubmed/36573287
http://dx.doi.org/10.1111/os.13622
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