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A bacterial outer membrane vesicle-based click vaccine elicits potent immune response against Staphylococcus aureus in mice

Staphylococcus aureus infection is a severe public health concern with the growing number of multidrug-resistant strains. S. aureus can circumvent the defense mechanisms of host immunity with the aid of multiple virulence factors. An efficacious multicomponent vaccine targeting diverse immune evasio...

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Autores principales: Sun, Jingjing, Lin, Xuansheng, He, Yige, Zhang, Baozhong, Zhou, Nan, Huang, Jian-dong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9892643/
https://www.ncbi.nlm.nih.gov/pubmed/36742310
http://dx.doi.org/10.3389/fimmu.2023.1088501
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author Sun, Jingjing
Lin, Xuansheng
He, Yige
Zhang, Baozhong
Zhou, Nan
Huang, Jian-dong
author_facet Sun, Jingjing
Lin, Xuansheng
He, Yige
Zhang, Baozhong
Zhou, Nan
Huang, Jian-dong
author_sort Sun, Jingjing
collection PubMed
description Staphylococcus aureus infection is a severe public health concern with the growing number of multidrug-resistant strains. S. aureus can circumvent the defense mechanisms of host immunity with the aid of multiple virulence factors. An efficacious multicomponent vaccine targeting diverse immune evasion strategies developed by S. aureus is thus crucial for its infection control. In this study, we exploited the SpyCatcher-SpyTag system to engineer bacterial outer membrane vesicles (OMVs) for the development of a multitargeting S. aureus click vaccine. We decorated OMVs with surface exposed SpyCatcher via a truncated OmpA(a.a 1-155)-SpyCatcher fusion. The engineered OMVs can flexibly bind with various SpyTag-fused S. aureus antigens to generate an OMV-based click vaccine. Compared with antigens mixed with alum adjuvant, the click vaccine simultaneously induced more potent antigen-specific humoral and Th1-based cellular immune response, which afforded protection against S. aureus Newman lethal challenge in a mouse model. Our study provided a flexible and versatile click vaccine strategy with the potential for fighting against emerging S. aureus clinical isolates.
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spelling pubmed-98926432023-02-03 A bacterial outer membrane vesicle-based click vaccine elicits potent immune response against Staphylococcus aureus in mice Sun, Jingjing Lin, Xuansheng He, Yige Zhang, Baozhong Zhou, Nan Huang, Jian-dong Front Immunol Immunology Staphylococcus aureus infection is a severe public health concern with the growing number of multidrug-resistant strains. S. aureus can circumvent the defense mechanisms of host immunity with the aid of multiple virulence factors. An efficacious multicomponent vaccine targeting diverse immune evasion strategies developed by S. aureus is thus crucial for its infection control. In this study, we exploited the SpyCatcher-SpyTag system to engineer bacterial outer membrane vesicles (OMVs) for the development of a multitargeting S. aureus click vaccine. We decorated OMVs with surface exposed SpyCatcher via a truncated OmpA(a.a 1-155)-SpyCatcher fusion. The engineered OMVs can flexibly bind with various SpyTag-fused S. aureus antigens to generate an OMV-based click vaccine. Compared with antigens mixed with alum adjuvant, the click vaccine simultaneously induced more potent antigen-specific humoral and Th1-based cellular immune response, which afforded protection against S. aureus Newman lethal challenge in a mouse model. Our study provided a flexible and versatile click vaccine strategy with the potential for fighting against emerging S. aureus clinical isolates. Frontiers Media S.A. 2023-01-19 /pmc/articles/PMC9892643/ /pubmed/36742310 http://dx.doi.org/10.3389/fimmu.2023.1088501 Text en Copyright © 2023 Sun, Lin, He, Zhang, Zhou and Huang https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Sun, Jingjing
Lin, Xuansheng
He, Yige
Zhang, Baozhong
Zhou, Nan
Huang, Jian-dong
A bacterial outer membrane vesicle-based click vaccine elicits potent immune response against Staphylococcus aureus in mice
title A bacterial outer membrane vesicle-based click vaccine elicits potent immune response against Staphylococcus aureus in mice
title_full A bacterial outer membrane vesicle-based click vaccine elicits potent immune response against Staphylococcus aureus in mice
title_fullStr A bacterial outer membrane vesicle-based click vaccine elicits potent immune response against Staphylococcus aureus in mice
title_full_unstemmed A bacterial outer membrane vesicle-based click vaccine elicits potent immune response against Staphylococcus aureus in mice
title_short A bacterial outer membrane vesicle-based click vaccine elicits potent immune response against Staphylococcus aureus in mice
title_sort bacterial outer membrane vesicle-based click vaccine elicits potent immune response against staphylococcus aureus in mice
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9892643/
https://www.ncbi.nlm.nih.gov/pubmed/36742310
http://dx.doi.org/10.3389/fimmu.2023.1088501
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