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Recipe for pituitary organoids

Generation of a variety of organs and tissues from human pluripotent stem cells (hPSCs) has been attempted in vitro. We here present a simple and efficient method for induction of hypothalamic and pituitary tissues from hPSCs. On provision of exogenous agents important for early hypothalamus-pituita...

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Autores principales: Kano, Mayuko, Sasaki, Hiroo, Miwata, Tsutomu, Suga, Hidetaka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9892717/
https://www.ncbi.nlm.nih.gov/pubmed/36743928
http://dx.doi.org/10.3389/fendo.2022.1025825
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author Kano, Mayuko
Sasaki, Hiroo
Miwata, Tsutomu
Suga, Hidetaka
author_facet Kano, Mayuko
Sasaki, Hiroo
Miwata, Tsutomu
Suga, Hidetaka
author_sort Kano, Mayuko
collection PubMed
description Generation of a variety of organs and tissues from human pluripotent stem cells (hPSCs) has been attempted in vitro. We here present a simple and efficient method for induction of hypothalamic and pituitary tissues from hPSCs. On provision of exogenous agents important for early hypothalamus-pituitary organogenesis, including bone morphogenetic protein 4 and activators of sonic hedgehog, in three-dimensional culture, hPSCs spontaneously form spherical organoids with two distinct tissues, hypothalamus and adenohypophysis. The pituitary tissues derived from hPSCs not only secrete adenocorticotropic hormone, but also retain both positive and negative feedback mechanisms, recapitulating mature endocrine organs in vivo. Furthermore, the results of ectopic transplantation with mouse models of hypopituitarism suggest that these hypothalamus-pituitary organoids have potential as engraftment organs. In addition to their use in transplantation for patients with hypopituitarism they will allow establishment of disease models in vitro and enable research impossible in humans. Hypothalamus-pituitary organoids promise to be a powerful tool in regenerative medicine, drug discovery, and basic research into pituitary development.
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spelling pubmed-98927172023-02-03 Recipe for pituitary organoids Kano, Mayuko Sasaki, Hiroo Miwata, Tsutomu Suga, Hidetaka Front Endocrinol (Lausanne) Endocrinology Generation of a variety of organs and tissues from human pluripotent stem cells (hPSCs) has been attempted in vitro. We here present a simple and efficient method for induction of hypothalamic and pituitary tissues from hPSCs. On provision of exogenous agents important for early hypothalamus-pituitary organogenesis, including bone morphogenetic protein 4 and activators of sonic hedgehog, in three-dimensional culture, hPSCs spontaneously form spherical organoids with two distinct tissues, hypothalamus and adenohypophysis. The pituitary tissues derived from hPSCs not only secrete adenocorticotropic hormone, but also retain both positive and negative feedback mechanisms, recapitulating mature endocrine organs in vivo. Furthermore, the results of ectopic transplantation with mouse models of hypopituitarism suggest that these hypothalamus-pituitary organoids have potential as engraftment organs. In addition to their use in transplantation for patients with hypopituitarism they will allow establishment of disease models in vitro and enable research impossible in humans. Hypothalamus-pituitary organoids promise to be a powerful tool in regenerative medicine, drug discovery, and basic research into pituitary development. Frontiers Media S.A. 2023-01-19 /pmc/articles/PMC9892717/ /pubmed/36743928 http://dx.doi.org/10.3389/fendo.2022.1025825 Text en Copyright © 2023 Kano, Sasaki, Miwata and Suga https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Endocrinology
Kano, Mayuko
Sasaki, Hiroo
Miwata, Tsutomu
Suga, Hidetaka
Recipe for pituitary organoids
title Recipe for pituitary organoids
title_full Recipe for pituitary organoids
title_fullStr Recipe for pituitary organoids
title_full_unstemmed Recipe for pituitary organoids
title_short Recipe for pituitary organoids
title_sort recipe for pituitary organoids
topic Endocrinology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9892717/
https://www.ncbi.nlm.nih.gov/pubmed/36743928
http://dx.doi.org/10.3389/fendo.2022.1025825
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