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Recipe for pituitary organoids
Generation of a variety of organs and tissues from human pluripotent stem cells (hPSCs) has been attempted in vitro. We here present a simple and efficient method for induction of hypothalamic and pituitary tissues from hPSCs. On provision of exogenous agents important for early hypothalamus-pituita...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9892717/ https://www.ncbi.nlm.nih.gov/pubmed/36743928 http://dx.doi.org/10.3389/fendo.2022.1025825 |
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author | Kano, Mayuko Sasaki, Hiroo Miwata, Tsutomu Suga, Hidetaka |
author_facet | Kano, Mayuko Sasaki, Hiroo Miwata, Tsutomu Suga, Hidetaka |
author_sort | Kano, Mayuko |
collection | PubMed |
description | Generation of a variety of organs and tissues from human pluripotent stem cells (hPSCs) has been attempted in vitro. We here present a simple and efficient method for induction of hypothalamic and pituitary tissues from hPSCs. On provision of exogenous agents important for early hypothalamus-pituitary organogenesis, including bone morphogenetic protein 4 and activators of sonic hedgehog, in three-dimensional culture, hPSCs spontaneously form spherical organoids with two distinct tissues, hypothalamus and adenohypophysis. The pituitary tissues derived from hPSCs not only secrete adenocorticotropic hormone, but also retain both positive and negative feedback mechanisms, recapitulating mature endocrine organs in vivo. Furthermore, the results of ectopic transplantation with mouse models of hypopituitarism suggest that these hypothalamus-pituitary organoids have potential as engraftment organs. In addition to their use in transplantation for patients with hypopituitarism they will allow establishment of disease models in vitro and enable research impossible in humans. Hypothalamus-pituitary organoids promise to be a powerful tool in regenerative medicine, drug discovery, and basic research into pituitary development. |
format | Online Article Text |
id | pubmed-9892717 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-98927172023-02-03 Recipe for pituitary organoids Kano, Mayuko Sasaki, Hiroo Miwata, Tsutomu Suga, Hidetaka Front Endocrinol (Lausanne) Endocrinology Generation of a variety of organs and tissues from human pluripotent stem cells (hPSCs) has been attempted in vitro. We here present a simple and efficient method for induction of hypothalamic and pituitary tissues from hPSCs. On provision of exogenous agents important for early hypothalamus-pituitary organogenesis, including bone morphogenetic protein 4 and activators of sonic hedgehog, in three-dimensional culture, hPSCs spontaneously form spherical organoids with two distinct tissues, hypothalamus and adenohypophysis. The pituitary tissues derived from hPSCs not only secrete adenocorticotropic hormone, but also retain both positive and negative feedback mechanisms, recapitulating mature endocrine organs in vivo. Furthermore, the results of ectopic transplantation with mouse models of hypopituitarism suggest that these hypothalamus-pituitary organoids have potential as engraftment organs. In addition to their use in transplantation for patients with hypopituitarism they will allow establishment of disease models in vitro and enable research impossible in humans. Hypothalamus-pituitary organoids promise to be a powerful tool in regenerative medicine, drug discovery, and basic research into pituitary development. Frontiers Media S.A. 2023-01-19 /pmc/articles/PMC9892717/ /pubmed/36743928 http://dx.doi.org/10.3389/fendo.2022.1025825 Text en Copyright © 2023 Kano, Sasaki, Miwata and Suga https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Endocrinology Kano, Mayuko Sasaki, Hiroo Miwata, Tsutomu Suga, Hidetaka Recipe for pituitary organoids |
title | Recipe for pituitary organoids |
title_full | Recipe for pituitary organoids |
title_fullStr | Recipe for pituitary organoids |
title_full_unstemmed | Recipe for pituitary organoids |
title_short | Recipe for pituitary organoids |
title_sort | recipe for pituitary organoids |
topic | Endocrinology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9892717/ https://www.ncbi.nlm.nih.gov/pubmed/36743928 http://dx.doi.org/10.3389/fendo.2022.1025825 |
work_keys_str_mv | AT kanomayuko recipeforpituitaryorganoids AT sasakihiroo recipeforpituitaryorganoids AT miwatatsutomu recipeforpituitaryorganoids AT sugahidetaka recipeforpituitaryorganoids |