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Combining high carotenoid, grain protein content and rust resistance in wheat for food and nutritional security
Globally, malnutrition has given birth to an alarming predicament, especially in developing countries, and has extensively shifted consumer preferences from conventional high-energy diets to a nutritionally balanced, cost-effective, sustainable, and healthy lifestyle. In keeping with this view and t...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9893017/ https://www.ncbi.nlm.nih.gov/pubmed/36741327 http://dx.doi.org/10.3389/fgene.2023.1075767 |
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author | Padhy, Asish Kumar Sharma, Achla Sharma, Himanshu Srivastava, Puja Singh, Satinder Kaur, Parampreet Kaur, Jaspal Kaur, Satinder Chhuneja, Parveen Bains, Navtej Singh |
author_facet | Padhy, Asish Kumar Sharma, Achla Sharma, Himanshu Srivastava, Puja Singh, Satinder Kaur, Parampreet Kaur, Jaspal Kaur, Satinder Chhuneja, Parveen Bains, Navtej Singh |
author_sort | Padhy, Asish Kumar |
collection | PubMed |
description | Globally, malnutrition has given birth to an alarming predicament, especially in developing countries, and has extensively shifted consumer preferences from conventional high-energy diets to a nutritionally balanced, cost-effective, sustainable, and healthy lifestyle. In keeping with this view and the mandate for developing high-yielding, disease-resistant biofortified staple food (wheat) for catering to the demand-driven market, the current research aimed at stacking together the enhanced grain protein content, carotenoid content, and disease resistance in an elite bread wheat background. The Y gene (PsyE1) and the GpcB1 gene were used as novel sources for enhancing the grain carotenoid and protein content in the commercial elite bread wheat cultivar HD2967. The combination also led to the stacking of resistance against all three foliar rusts owing to linked resistance genes. A stepwise hybridization using Parent 1 (HD2967 + PsyE1/Lr19/Sr25) with Parent 2 (PBW550 + GpcB1/Yr36+ Yr15), coupled with a phenotypic-biochemical selection, narrowed down 2748 F(2) individuals to a subset of 649 F(2) plants for molecular screening. The gene-specific markers PsyE1, PsyD1, Xucw108, and Xbarc8 for the genes PsyE1, PsyD1, GpcB1, and Yr15, respectively, were employed for forward selection. Four bread wheat lines positive for all the desired genes with high carotenoid (>8ppm) and protein (>13%) content were raised to the F(5) generation and will be evaluated for yield potential after bulking. These improved advanced breeding lines developed following multipronged efforts should prove a valuable and unique source for the development of cultivars with improved nutritional quality and rust resistance in wheat breeding programs. |
format | Online Article Text |
id | pubmed-9893017 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-98930172023-02-03 Combining high carotenoid, grain protein content and rust resistance in wheat for food and nutritional security Padhy, Asish Kumar Sharma, Achla Sharma, Himanshu Srivastava, Puja Singh, Satinder Kaur, Parampreet Kaur, Jaspal Kaur, Satinder Chhuneja, Parveen Bains, Navtej Singh Front Genet Genetics Globally, malnutrition has given birth to an alarming predicament, especially in developing countries, and has extensively shifted consumer preferences from conventional high-energy diets to a nutritionally balanced, cost-effective, sustainable, and healthy lifestyle. In keeping with this view and the mandate for developing high-yielding, disease-resistant biofortified staple food (wheat) for catering to the demand-driven market, the current research aimed at stacking together the enhanced grain protein content, carotenoid content, and disease resistance in an elite bread wheat background. The Y gene (PsyE1) and the GpcB1 gene were used as novel sources for enhancing the grain carotenoid and protein content in the commercial elite bread wheat cultivar HD2967. The combination also led to the stacking of resistance against all three foliar rusts owing to linked resistance genes. A stepwise hybridization using Parent 1 (HD2967 + PsyE1/Lr19/Sr25) with Parent 2 (PBW550 + GpcB1/Yr36+ Yr15), coupled with a phenotypic-biochemical selection, narrowed down 2748 F(2) individuals to a subset of 649 F(2) plants for molecular screening. The gene-specific markers PsyE1, PsyD1, Xucw108, and Xbarc8 for the genes PsyE1, PsyD1, GpcB1, and Yr15, respectively, were employed for forward selection. Four bread wheat lines positive for all the desired genes with high carotenoid (>8ppm) and protein (>13%) content were raised to the F(5) generation and will be evaluated for yield potential after bulking. These improved advanced breeding lines developed following multipronged efforts should prove a valuable and unique source for the development of cultivars with improved nutritional quality and rust resistance in wheat breeding programs. Frontiers Media S.A. 2023-01-19 /pmc/articles/PMC9893017/ /pubmed/36741327 http://dx.doi.org/10.3389/fgene.2023.1075767 Text en Copyright © 2023 Padhy, Sharma, Sharma, Srivastava, Singh, Kaur, Kaur, Kaur, Chhuneja and Bains. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Genetics Padhy, Asish Kumar Sharma, Achla Sharma, Himanshu Srivastava, Puja Singh, Satinder Kaur, Parampreet Kaur, Jaspal Kaur, Satinder Chhuneja, Parveen Bains, Navtej Singh Combining high carotenoid, grain protein content and rust resistance in wheat for food and nutritional security |
title | Combining high carotenoid, grain protein content and rust resistance in wheat for food and nutritional security |
title_full | Combining high carotenoid, grain protein content and rust resistance in wheat for food and nutritional security |
title_fullStr | Combining high carotenoid, grain protein content and rust resistance in wheat for food and nutritional security |
title_full_unstemmed | Combining high carotenoid, grain protein content and rust resistance in wheat for food and nutritional security |
title_short | Combining high carotenoid, grain protein content and rust resistance in wheat for food and nutritional security |
title_sort | combining high carotenoid, grain protein content and rust resistance in wheat for food and nutritional security |
topic | Genetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9893017/ https://www.ncbi.nlm.nih.gov/pubmed/36741327 http://dx.doi.org/10.3389/fgene.2023.1075767 |
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