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Using the inner membrane of Escherichia coli as a scaffold to anchor enzymes for metabolic flux enhancement

Clustering enzymes in the same metabolic pathway is a natural strategy to enhance productivity. Synthetic protein, RNA and DNA scaffolds have been designed to artificially cluster multiple enzymes in the cell, which require complex construction processes and possess limited slots for target enzymes....

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Autores principales: Wang, You, Wang, Yushu, Wu, Yuqi, Suo, Yang, Guo, Huaqing, Yu, Yineng, Yin, Ruonan, Xi, Rui, Wu, Jiajie, Hua, Nan, Zhang, Yuehan, Zhang, Shaobo, Jin, Zhenming, He, Lin, Ma, Gang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9893748/
https://www.ncbi.nlm.nih.gov/pubmed/36751472
http://dx.doi.org/10.1002/elsc.202200034
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author Wang, You
Wang, Yushu
Wu, Yuqi
Suo, Yang
Guo, Huaqing
Yu, Yineng
Yin, Ruonan
Xi, Rui
Wu, Jiajie
Hua, Nan
Zhang, Yuehan
Zhang, Shaobo
Jin, Zhenming
He, Lin
Ma, Gang
author_facet Wang, You
Wang, Yushu
Wu, Yuqi
Suo, Yang
Guo, Huaqing
Yu, Yineng
Yin, Ruonan
Xi, Rui
Wu, Jiajie
Hua, Nan
Zhang, Yuehan
Zhang, Shaobo
Jin, Zhenming
He, Lin
Ma, Gang
author_sort Wang, You
collection PubMed
description Clustering enzymes in the same metabolic pathway is a natural strategy to enhance productivity. Synthetic protein, RNA and DNA scaffolds have been designed to artificially cluster multiple enzymes in the cell, which require complex construction processes and possess limited slots for target enzymes. We utilized the Escherichia coli inner cell membrane as a native scaffold to cluster four fatty acid synthases (FAS) and achieved to improve the efficiency of fatty acid synthesis in vivo. The construction strategy is as simple as fusing target enzymes to the N‐terminus or C‐terminus of the membrane anchor protein (Lgt), and the number of anchored enzymes is not restricted. This novel device not only presents a similar efficiency in clustering multiple enzymes to that of other artificial scaffolds but also promotes the product secretion, driving the entire metabolic flux forward and further increasing the gross yield compared with that in a cytoplasmic scaffold system.
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spelling pubmed-98937482023-02-06 Using the inner membrane of Escherichia coli as a scaffold to anchor enzymes for metabolic flux enhancement Wang, You Wang, Yushu Wu, Yuqi Suo, Yang Guo, Huaqing Yu, Yineng Yin, Ruonan Xi, Rui Wu, Jiajie Hua, Nan Zhang, Yuehan Zhang, Shaobo Jin, Zhenming He, Lin Ma, Gang Eng Life Sci Research Articles Clustering enzymes in the same metabolic pathway is a natural strategy to enhance productivity. Synthetic protein, RNA and DNA scaffolds have been designed to artificially cluster multiple enzymes in the cell, which require complex construction processes and possess limited slots for target enzymes. We utilized the Escherichia coli inner cell membrane as a native scaffold to cluster four fatty acid synthases (FAS) and achieved to improve the efficiency of fatty acid synthesis in vivo. The construction strategy is as simple as fusing target enzymes to the N‐terminus or C‐terminus of the membrane anchor protein (Lgt), and the number of anchored enzymes is not restricted. This novel device not only presents a similar efficiency in clustering multiple enzymes to that of other artificial scaffolds but also promotes the product secretion, driving the entire metabolic flux forward and further increasing the gross yield compared with that in a cytoplasmic scaffold system. John Wiley and Sons Inc. 2023-01-10 /pmc/articles/PMC9893748/ /pubmed/36751472 http://dx.doi.org/10.1002/elsc.202200034 Text en © 2022 The Authors. Engineering in Life Sciences published by Wiley‐VCH GmbH https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Wang, You
Wang, Yushu
Wu, Yuqi
Suo, Yang
Guo, Huaqing
Yu, Yineng
Yin, Ruonan
Xi, Rui
Wu, Jiajie
Hua, Nan
Zhang, Yuehan
Zhang, Shaobo
Jin, Zhenming
He, Lin
Ma, Gang
Using the inner membrane of Escherichia coli as a scaffold to anchor enzymes for metabolic flux enhancement
title Using the inner membrane of Escherichia coli as a scaffold to anchor enzymes for metabolic flux enhancement
title_full Using the inner membrane of Escherichia coli as a scaffold to anchor enzymes for metabolic flux enhancement
title_fullStr Using the inner membrane of Escherichia coli as a scaffold to anchor enzymes for metabolic flux enhancement
title_full_unstemmed Using the inner membrane of Escherichia coli as a scaffold to anchor enzymes for metabolic flux enhancement
title_short Using the inner membrane of Escherichia coli as a scaffold to anchor enzymes for metabolic flux enhancement
title_sort using the inner membrane of escherichia coli as a scaffold to anchor enzymes for metabolic flux enhancement
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9893748/
https://www.ncbi.nlm.nih.gov/pubmed/36751472
http://dx.doi.org/10.1002/elsc.202200034
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