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Enrichment of homoacetogens converting H(2)/CO(2) into acids and ethanol and simultaneous methane production

An anaerobic granular sludge was enriched to utilize H(2)/CO(2) in a continuous gas‐fed up‐flow anaerobic sludge reactor by applying operating conditions expected to produce acetic acid, butyric acid, and ethanol. Three stages of fermentation were found: Stage I with acetic acid accumulation with th...

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Autores principales: He, Yaxue, Cassarini, Chiara, Lens, Piet N.L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9893759/
https://www.ncbi.nlm.nih.gov/pubmed/36751471
http://dx.doi.org/10.1002/elsc.202200027
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author He, Yaxue
Cassarini, Chiara
Lens, Piet N.L.
author_facet He, Yaxue
Cassarini, Chiara
Lens, Piet N.L.
author_sort He, Yaxue
collection PubMed
description An anaerobic granular sludge was enriched to utilize H(2)/CO(2) in a continuous gas‐fed up‐flow anaerobic sludge reactor by applying operating conditions expected to produce acetic acid, butyric acid, and ethanol. Three stages of fermentation were found: Stage I with acetic acid accumulation with the highest concentration of 35 mM along with a pH decrease from initial 6 to 4.5. In Stage II, H(2)/CO(2) was replaced by 100% H(2) to induce solventogenesis, whereas butyric acid was produced with the highest concentration of 2.5 mM. At stage III with 10 µM tungsten (W) addition, iso‐valeric acid, valeric acid, and caproic acid were produced at pH 4.5–5.0. In the batch tests inoculated with the enriched sludge taken from the bioreactor (day 70), however, methane production occurred at pH 6. Exogenous 15 mM acetate addition enhanced both the H(2) and CO(2) consumption rate compared to exogenous 10, 30, and 45 mM acetate by the enriched sludge. Exogenous acetate was failed to be converted to ethanol using H(2) as electron donor by the enriched acetogens.
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spelling pubmed-98937592023-02-06 Enrichment of homoacetogens converting H(2)/CO(2) into acids and ethanol and simultaneous methane production He, Yaxue Cassarini, Chiara Lens, Piet N.L. Eng Life Sci Research Articles An anaerobic granular sludge was enriched to utilize H(2)/CO(2) in a continuous gas‐fed up‐flow anaerobic sludge reactor by applying operating conditions expected to produce acetic acid, butyric acid, and ethanol. Three stages of fermentation were found: Stage I with acetic acid accumulation with the highest concentration of 35 mM along with a pH decrease from initial 6 to 4.5. In Stage II, H(2)/CO(2) was replaced by 100% H(2) to induce solventogenesis, whereas butyric acid was produced with the highest concentration of 2.5 mM. At stage III with 10 µM tungsten (W) addition, iso‐valeric acid, valeric acid, and caproic acid were produced at pH 4.5–5.0. In the batch tests inoculated with the enriched sludge taken from the bioreactor (day 70), however, methane production occurred at pH 6. Exogenous 15 mM acetate addition enhanced both the H(2) and CO(2) consumption rate compared to exogenous 10, 30, and 45 mM acetate by the enriched sludge. Exogenous acetate was failed to be converted to ethanol using H(2) as electron donor by the enriched acetogens. John Wiley and Sons Inc. 2022-12-07 /pmc/articles/PMC9893759/ /pubmed/36751471 http://dx.doi.org/10.1002/elsc.202200027 Text en © 2022 The Authors. Engineering in Life Sciences published by Wiley‐VCH GmbH https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
He, Yaxue
Cassarini, Chiara
Lens, Piet N.L.
Enrichment of homoacetogens converting H(2)/CO(2) into acids and ethanol and simultaneous methane production
title Enrichment of homoacetogens converting H(2)/CO(2) into acids and ethanol and simultaneous methane production
title_full Enrichment of homoacetogens converting H(2)/CO(2) into acids and ethanol and simultaneous methane production
title_fullStr Enrichment of homoacetogens converting H(2)/CO(2) into acids and ethanol and simultaneous methane production
title_full_unstemmed Enrichment of homoacetogens converting H(2)/CO(2) into acids and ethanol and simultaneous methane production
title_short Enrichment of homoacetogens converting H(2)/CO(2) into acids and ethanol and simultaneous methane production
title_sort enrichment of homoacetogens converting h(2)/co(2) into acids and ethanol and simultaneous methane production
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9893759/
https://www.ncbi.nlm.nih.gov/pubmed/36751471
http://dx.doi.org/10.1002/elsc.202200027
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