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Tipping the scales of understanding: An engineering approach to design and implement whole-body cardiac electrophysiology experimental models
The study of cardiac electrophysiology is built on experimental models that span all scales, from ion channels to whole-body preparations. Novel discoveries made at each scale have contributed to our fundamental understanding of human cardiac electrophysiology, which informs clinicians as they detec...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9893785/ https://www.ncbi.nlm.nih.gov/pubmed/36744034 http://dx.doi.org/10.3389/fphys.2023.1100471 |
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author | Zenger, Brian Bergquist, Jake A. Busatto, Anna Good, Wilson W. Rupp, Lindsay C. Sharma, Vikas MacLeod, Rob S. |
author_facet | Zenger, Brian Bergquist, Jake A. Busatto, Anna Good, Wilson W. Rupp, Lindsay C. Sharma, Vikas MacLeod, Rob S. |
author_sort | Zenger, Brian |
collection | PubMed |
description | The study of cardiac electrophysiology is built on experimental models that span all scales, from ion channels to whole-body preparations. Novel discoveries made at each scale have contributed to our fundamental understanding of human cardiac electrophysiology, which informs clinicians as they detect, diagnose, and treat complex cardiac pathologies. This expert review describes an engineering approach to developing experimental models that is applicable across scales. The review also outlines how we applied the approach to create a set of multiscale whole-body experimental models of cardiac electrophysiology, models that are driving new insights into the response of the myocardium to acute ischemia. Specifically, we propose that researchers must address three critical requirements to develop an effective experimental model: 1) how the experimental model replicates and maintains human physiological conditions, 2) how the interventions possible with the experimental model capture human pathophysiology, and 3) what signals need to be measured, at which levels of resolution and fidelity, and what are the resulting requirements of the measurement system and the access to the organs of interest. We will discuss these requirements in the context of two examples of whole-body experimental models, a closed chest in situ model of cardiac ischemia and an isolated-heart, torso-tank preparation, both of which we have developed over decades and used to gather valuable insights from hundreds of experiments. |
format | Online Article Text |
id | pubmed-9893785 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-98937852023-02-03 Tipping the scales of understanding: An engineering approach to design and implement whole-body cardiac electrophysiology experimental models Zenger, Brian Bergquist, Jake A. Busatto, Anna Good, Wilson W. Rupp, Lindsay C. Sharma, Vikas MacLeod, Rob S. Front Physiol Physiology The study of cardiac electrophysiology is built on experimental models that span all scales, from ion channels to whole-body preparations. Novel discoveries made at each scale have contributed to our fundamental understanding of human cardiac electrophysiology, which informs clinicians as they detect, diagnose, and treat complex cardiac pathologies. This expert review describes an engineering approach to developing experimental models that is applicable across scales. The review also outlines how we applied the approach to create a set of multiscale whole-body experimental models of cardiac electrophysiology, models that are driving new insights into the response of the myocardium to acute ischemia. Specifically, we propose that researchers must address three critical requirements to develop an effective experimental model: 1) how the experimental model replicates and maintains human physiological conditions, 2) how the interventions possible with the experimental model capture human pathophysiology, and 3) what signals need to be measured, at which levels of resolution and fidelity, and what are the resulting requirements of the measurement system and the access to the organs of interest. We will discuss these requirements in the context of two examples of whole-body experimental models, a closed chest in situ model of cardiac ischemia and an isolated-heart, torso-tank preparation, both of which we have developed over decades and used to gather valuable insights from hundreds of experiments. Frontiers Media S.A. 2023-01-19 /pmc/articles/PMC9893785/ /pubmed/36744034 http://dx.doi.org/10.3389/fphys.2023.1100471 Text en Copyright © 2023 Zenger, Bergquist, Busatto, Good, Rupp, Sharma and MacLeod. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Physiology Zenger, Brian Bergquist, Jake A. Busatto, Anna Good, Wilson W. Rupp, Lindsay C. Sharma, Vikas MacLeod, Rob S. Tipping the scales of understanding: An engineering approach to design and implement whole-body cardiac electrophysiology experimental models |
title | Tipping the scales of understanding: An engineering approach to design and implement whole-body cardiac electrophysiology experimental models |
title_full | Tipping the scales of understanding: An engineering approach to design and implement whole-body cardiac electrophysiology experimental models |
title_fullStr | Tipping the scales of understanding: An engineering approach to design and implement whole-body cardiac electrophysiology experimental models |
title_full_unstemmed | Tipping the scales of understanding: An engineering approach to design and implement whole-body cardiac electrophysiology experimental models |
title_short | Tipping the scales of understanding: An engineering approach to design and implement whole-body cardiac electrophysiology experimental models |
title_sort | tipping the scales of understanding: an engineering approach to design and implement whole-body cardiac electrophysiology experimental models |
topic | Physiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9893785/ https://www.ncbi.nlm.nih.gov/pubmed/36744034 http://dx.doi.org/10.3389/fphys.2023.1100471 |
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