Cargando…

Multimodal single-cell analyses of peripheral blood mononuclear cells of COVID-19 patients in Japan

SARS-CoV-2 continues to spread worldwide. Patients with COVID-19 show distinct clinical symptoms. Although many studies have reported various causes for the diversity of symptoms, the underlying mechanisms are not fully understood. Peripheral blood mononuclear cells from COVID-19 patients were colle...

Descripción completa

Detalles Bibliográficos
Autores principales: Kashima, Yukie, Mizutani, Taketoshi, Nakayama-Hosoya, Kaori, Moriyama, Saya, Matsumura, Takayuki, Yoshimura, Yoshihiro, Sasaki, Hiroaki, Horiuchi, Hiroshi, Miyata, Nobuyuki, Miyazaki, Kazuhito, Tachikawa, Natsuo, Takahashi, Yoshimasa, Suzuki, Tadaki, Sugano, Sumio, Matano, Tetsuro, Kawana-Tachikawa, Ai, Suzuki, Yutaka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9893982/
https://www.ncbi.nlm.nih.gov/pubmed/36732528
http://dx.doi.org/10.1038/s41598-023-28696-9
_version_ 1784881640744419328
author Kashima, Yukie
Mizutani, Taketoshi
Nakayama-Hosoya, Kaori
Moriyama, Saya
Matsumura, Takayuki
Yoshimura, Yoshihiro
Sasaki, Hiroaki
Horiuchi, Hiroshi
Miyata, Nobuyuki
Miyazaki, Kazuhito
Tachikawa, Natsuo
Takahashi, Yoshimasa
Suzuki, Tadaki
Sugano, Sumio
Matano, Tetsuro
Kawana-Tachikawa, Ai
Suzuki, Yutaka
author_facet Kashima, Yukie
Mizutani, Taketoshi
Nakayama-Hosoya, Kaori
Moriyama, Saya
Matsumura, Takayuki
Yoshimura, Yoshihiro
Sasaki, Hiroaki
Horiuchi, Hiroshi
Miyata, Nobuyuki
Miyazaki, Kazuhito
Tachikawa, Natsuo
Takahashi, Yoshimasa
Suzuki, Tadaki
Sugano, Sumio
Matano, Tetsuro
Kawana-Tachikawa, Ai
Suzuki, Yutaka
author_sort Kashima, Yukie
collection PubMed
description SARS-CoV-2 continues to spread worldwide. Patients with COVID-19 show distinct clinical symptoms. Although many studies have reported various causes for the diversity of symptoms, the underlying mechanisms are not fully understood. Peripheral blood mononuclear cells from COVID-19 patients were collected longitudinally, and single-cell transcriptome and T cell receptor repertoire analysis was performed. Comparison of molecular features and patients’ clinical information revealed that the proportions of cells present, and gene expression profiles differed significantly between mild and severe cases; although even among severe cases, substantial differences were observed among the patients. In one severely-infected elderly patient, an effective antibody response seemed to have failed, which may have caused prolonged viral clearance. Naïve T cell depletion, low T cell receptor repertoire diversity, and aberrant hyperactivation of most immune cell subsets were observed during the acute phase in this patient. Through this study, we provided a better understanding of the diversity of immune landscapes and responses. The information obtained from this study can help medical professionals develop personalized optimal clinical treatment strategies for COVID-19.
format Online
Article
Text
id pubmed-9893982
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-98939822023-02-02 Multimodal single-cell analyses of peripheral blood mononuclear cells of COVID-19 patients in Japan Kashima, Yukie Mizutani, Taketoshi Nakayama-Hosoya, Kaori Moriyama, Saya Matsumura, Takayuki Yoshimura, Yoshihiro Sasaki, Hiroaki Horiuchi, Hiroshi Miyata, Nobuyuki Miyazaki, Kazuhito Tachikawa, Natsuo Takahashi, Yoshimasa Suzuki, Tadaki Sugano, Sumio Matano, Tetsuro Kawana-Tachikawa, Ai Suzuki, Yutaka Sci Rep Article SARS-CoV-2 continues to spread worldwide. Patients with COVID-19 show distinct clinical symptoms. Although many studies have reported various causes for the diversity of symptoms, the underlying mechanisms are not fully understood. Peripheral blood mononuclear cells from COVID-19 patients were collected longitudinally, and single-cell transcriptome and T cell receptor repertoire analysis was performed. Comparison of molecular features and patients’ clinical information revealed that the proportions of cells present, and gene expression profiles differed significantly between mild and severe cases; although even among severe cases, substantial differences were observed among the patients. In one severely-infected elderly patient, an effective antibody response seemed to have failed, which may have caused prolonged viral clearance. Naïve T cell depletion, low T cell receptor repertoire diversity, and aberrant hyperactivation of most immune cell subsets were observed during the acute phase in this patient. Through this study, we provided a better understanding of the diversity of immune landscapes and responses. The information obtained from this study can help medical professionals develop personalized optimal clinical treatment strategies for COVID-19. Nature Publishing Group UK 2023-02-02 /pmc/articles/PMC9893982/ /pubmed/36732528 http://dx.doi.org/10.1038/s41598-023-28696-9 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Kashima, Yukie
Mizutani, Taketoshi
Nakayama-Hosoya, Kaori
Moriyama, Saya
Matsumura, Takayuki
Yoshimura, Yoshihiro
Sasaki, Hiroaki
Horiuchi, Hiroshi
Miyata, Nobuyuki
Miyazaki, Kazuhito
Tachikawa, Natsuo
Takahashi, Yoshimasa
Suzuki, Tadaki
Sugano, Sumio
Matano, Tetsuro
Kawana-Tachikawa, Ai
Suzuki, Yutaka
Multimodal single-cell analyses of peripheral blood mononuclear cells of COVID-19 patients in Japan
title Multimodal single-cell analyses of peripheral blood mononuclear cells of COVID-19 patients in Japan
title_full Multimodal single-cell analyses of peripheral blood mononuclear cells of COVID-19 patients in Japan
title_fullStr Multimodal single-cell analyses of peripheral blood mononuclear cells of COVID-19 patients in Japan
title_full_unstemmed Multimodal single-cell analyses of peripheral blood mononuclear cells of COVID-19 patients in Japan
title_short Multimodal single-cell analyses of peripheral blood mononuclear cells of COVID-19 patients in Japan
title_sort multimodal single-cell analyses of peripheral blood mononuclear cells of covid-19 patients in japan
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9893982/
https://www.ncbi.nlm.nih.gov/pubmed/36732528
http://dx.doi.org/10.1038/s41598-023-28696-9
work_keys_str_mv AT kashimayukie multimodalsinglecellanalysesofperipheralbloodmononuclearcellsofcovid19patientsinjapan
AT mizutanitaketoshi multimodalsinglecellanalysesofperipheralbloodmononuclearcellsofcovid19patientsinjapan
AT nakayamahosoyakaori multimodalsinglecellanalysesofperipheralbloodmononuclearcellsofcovid19patientsinjapan
AT moriyamasaya multimodalsinglecellanalysesofperipheralbloodmononuclearcellsofcovid19patientsinjapan
AT matsumuratakayuki multimodalsinglecellanalysesofperipheralbloodmononuclearcellsofcovid19patientsinjapan
AT yoshimurayoshihiro multimodalsinglecellanalysesofperipheralbloodmononuclearcellsofcovid19patientsinjapan
AT sasakihiroaki multimodalsinglecellanalysesofperipheralbloodmononuclearcellsofcovid19patientsinjapan
AT horiuchihiroshi multimodalsinglecellanalysesofperipheralbloodmononuclearcellsofcovid19patientsinjapan
AT miyatanobuyuki multimodalsinglecellanalysesofperipheralbloodmononuclearcellsofcovid19patientsinjapan
AT miyazakikazuhito multimodalsinglecellanalysesofperipheralbloodmononuclearcellsofcovid19patientsinjapan
AT tachikawanatsuo multimodalsinglecellanalysesofperipheralbloodmononuclearcellsofcovid19patientsinjapan
AT takahashiyoshimasa multimodalsinglecellanalysesofperipheralbloodmononuclearcellsofcovid19patientsinjapan
AT suzukitadaki multimodalsinglecellanalysesofperipheralbloodmononuclearcellsofcovid19patientsinjapan
AT suganosumio multimodalsinglecellanalysesofperipheralbloodmononuclearcellsofcovid19patientsinjapan
AT matanotetsuro multimodalsinglecellanalysesofperipheralbloodmononuclearcellsofcovid19patientsinjapan
AT kawanatachikawaai multimodalsinglecellanalysesofperipheralbloodmononuclearcellsofcovid19patientsinjapan
AT suzukiyutaka multimodalsinglecellanalysesofperipheralbloodmononuclearcellsofcovid19patientsinjapan