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Harnessing Wolbachia cytoplasmic incompatibility alleles for confined gene drive: A modeling study
Wolbachia are maternally-inherited bacteria, which can spread rapidly in populations by manipulating reproduction. cifA and cifB are genes found in Wolbachia phage that are responsible for cytoplasmic incompatibility, the most common type of Wolbachia reproductive interference. In this phenomenon, n...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9894560/ https://www.ncbi.nlm.nih.gov/pubmed/36689491 http://dx.doi.org/10.1371/journal.pgen.1010591 |
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author | Li, Jiahe Champer, Jackson |
author_facet | Li, Jiahe Champer, Jackson |
author_sort | Li, Jiahe |
collection | PubMed |
description | Wolbachia are maternally-inherited bacteria, which can spread rapidly in populations by manipulating reproduction. cifA and cifB are genes found in Wolbachia phage that are responsible for cytoplasmic incompatibility, the most common type of Wolbachia reproductive interference. In this phenomenon, no viable offspring are produced when a male with both cifA and cifB (or just cifB in some systems) mates with a female lacking cifA. Utilizing this feature, we propose new types of toxin-antidote gene drives that can be constructed with only these two genes in an insect genome, instead of the whole Wolbachia bacteria. By using both mathematical and simulation models, we found that a drive containing cifA and cifB together creates a confined drive with a moderate to high introduction threshold. When introduced separately, they act as a self-limiting drive. We observed that the performance of these drives is substantially influenced by various ecological parameters and drive characteristics. Extending our models to continuous space, we found that the drive individual release distribution has a critical impact on drive persistence. Our results suggest that these new types of drives based on Wolbachia transgenes are safe and flexible candidates for genetic modification of populations. |
format | Online Article Text |
id | pubmed-9894560 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-98945602023-02-03 Harnessing Wolbachia cytoplasmic incompatibility alleles for confined gene drive: A modeling study Li, Jiahe Champer, Jackson PLoS Genet Research Article Wolbachia are maternally-inherited bacteria, which can spread rapidly in populations by manipulating reproduction. cifA and cifB are genes found in Wolbachia phage that are responsible for cytoplasmic incompatibility, the most common type of Wolbachia reproductive interference. In this phenomenon, no viable offspring are produced when a male with both cifA and cifB (or just cifB in some systems) mates with a female lacking cifA. Utilizing this feature, we propose new types of toxin-antidote gene drives that can be constructed with only these two genes in an insect genome, instead of the whole Wolbachia bacteria. By using both mathematical and simulation models, we found that a drive containing cifA and cifB together creates a confined drive with a moderate to high introduction threshold. When introduced separately, they act as a self-limiting drive. We observed that the performance of these drives is substantially influenced by various ecological parameters and drive characteristics. Extending our models to continuous space, we found that the drive individual release distribution has a critical impact on drive persistence. Our results suggest that these new types of drives based on Wolbachia transgenes are safe and flexible candidates for genetic modification of populations. Public Library of Science 2023-01-23 /pmc/articles/PMC9894560/ /pubmed/36689491 http://dx.doi.org/10.1371/journal.pgen.1010591 Text en © 2023 Li, Champer https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Li, Jiahe Champer, Jackson Harnessing Wolbachia cytoplasmic incompatibility alleles for confined gene drive: A modeling study |
title | Harnessing Wolbachia cytoplasmic incompatibility alleles for confined gene drive: A modeling study |
title_full | Harnessing Wolbachia cytoplasmic incompatibility alleles for confined gene drive: A modeling study |
title_fullStr | Harnessing Wolbachia cytoplasmic incompatibility alleles for confined gene drive: A modeling study |
title_full_unstemmed | Harnessing Wolbachia cytoplasmic incompatibility alleles for confined gene drive: A modeling study |
title_short | Harnessing Wolbachia cytoplasmic incompatibility alleles for confined gene drive: A modeling study |
title_sort | harnessing wolbachia cytoplasmic incompatibility alleles for confined gene drive: a modeling study |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9894560/ https://www.ncbi.nlm.nih.gov/pubmed/36689491 http://dx.doi.org/10.1371/journal.pgen.1010591 |
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