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Simultaneous detection and quantification of spike mRNA and protein in SARS-CoV-2 infected airway epithelium.
Visualizing and quantifying mRNA and its corresponding protein provides a unique perspective of gene expression at a single-molecule level. Here, we describe a method for differentiating primary cells for making airway epithelium and detecting SARS-CoV-2 Spike (S) mRNA and S protein in the paraforma...
Autores principales: | Jerome, Kailey, Sattar, Sarah, Mehedi, Masfique |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9894770/ https://www.ncbi.nlm.nih.gov/pubmed/36779029 http://dx.doi.org/10.1016/j.mex.2023.102050 |
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