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Volatile metabolome and floral transcriptome analyses reveal the volatile components of strongly fragrant progeny of Malus × robusta

Floral fragrance is an important trait that contributes to the ornamental properties and pollination of crabapple. However, research on the physiological and molecular biology of the floral volatile compounds of crabapple is rarely reported. In this study, metabolomic and transcriptomic analyses of...

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Autores principales: Li, Guofang, Liu, Jia, Zhang, He, Jia, Linguang, Liu, Youxian, Li, Jiuyang, Zhou, Shiwei, Wang, Pengjuan, Tan, Ming, Shao, Jianzhu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9895795/
https://www.ncbi.nlm.nih.gov/pubmed/36743501
http://dx.doi.org/10.3389/fpls.2023.1065219
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author Li, Guofang
Liu, Jia
Zhang, He
Jia, Linguang
Liu, Youxian
Li, Jiuyang
Zhou, Shiwei
Wang, Pengjuan
Tan, Ming
Shao, Jianzhu
author_facet Li, Guofang
Liu, Jia
Zhang, He
Jia, Linguang
Liu, Youxian
Li, Jiuyang
Zhou, Shiwei
Wang, Pengjuan
Tan, Ming
Shao, Jianzhu
author_sort Li, Guofang
collection PubMed
description Floral fragrance is an important trait that contributes to the ornamental properties and pollination of crabapple. However, research on the physiological and molecular biology of the floral volatile compounds of crabapple is rarely reported. In this study, metabolomic and transcriptomic analyses of the floral volatile compounds of standard Malus robusta flowers (Mr), and progeny with strongly and weakly fragrant flowers (SF and WF, respectively), were conducted. Fifty-six floral volatile compounds were detected in the plant materials, mainly comprising phenylpropane/benzene ring-type compounds, fatty acid derivatives, and terpene compounds. The volatile contents were significantly increased before the early flowering stage (ES), and the contents of SF flowers were twice those of WF and Mr flowers. Odor activity values were determined for known fragrant volatiles and 10–11 key fragrant volatiles were identified at the ES. The predominant fragrant volatiles were methyl benzoate, linalool, leaf acetate, and methyl anthranilate. In the petals, stamens, pistil, and calyx of SF flowers, 26 volatiles were detected at the ES, among which phenylpropane/benzene ring-type compounds were the main components accounting for more than 75% of the total volatile content. Functional analysis of transcriptome data revealed that the phenylpropanoid biosynthesis pathway was significantly enriched in SF flowers. By conducting combined analyses between volatiles and differentially expressed genes, transcripts of six floral scent-related genes were identified and were associated with the contents of the key fragrant volatiles, and other 23 genes were potentially correlated with the key volatile compounds. The results reveal possible mechanisms for the emission of strong fragrance by SF flowers, and provide a foundation for improvement of the floral fragrance and development of new crabapple cultivars.
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spelling pubmed-98957952023-02-04 Volatile metabolome and floral transcriptome analyses reveal the volatile components of strongly fragrant progeny of Malus × robusta Li, Guofang Liu, Jia Zhang, He Jia, Linguang Liu, Youxian Li, Jiuyang Zhou, Shiwei Wang, Pengjuan Tan, Ming Shao, Jianzhu Front Plant Sci Plant Science Floral fragrance is an important trait that contributes to the ornamental properties and pollination of crabapple. However, research on the physiological and molecular biology of the floral volatile compounds of crabapple is rarely reported. In this study, metabolomic and transcriptomic analyses of the floral volatile compounds of standard Malus robusta flowers (Mr), and progeny with strongly and weakly fragrant flowers (SF and WF, respectively), were conducted. Fifty-six floral volatile compounds were detected in the plant materials, mainly comprising phenylpropane/benzene ring-type compounds, fatty acid derivatives, and terpene compounds. The volatile contents were significantly increased before the early flowering stage (ES), and the contents of SF flowers were twice those of WF and Mr flowers. Odor activity values were determined for known fragrant volatiles and 10–11 key fragrant volatiles were identified at the ES. The predominant fragrant volatiles were methyl benzoate, linalool, leaf acetate, and methyl anthranilate. In the petals, stamens, pistil, and calyx of SF flowers, 26 volatiles were detected at the ES, among which phenylpropane/benzene ring-type compounds were the main components accounting for more than 75% of the total volatile content. Functional analysis of transcriptome data revealed that the phenylpropanoid biosynthesis pathway was significantly enriched in SF flowers. By conducting combined analyses between volatiles and differentially expressed genes, transcripts of six floral scent-related genes were identified and were associated with the contents of the key fragrant volatiles, and other 23 genes were potentially correlated with the key volatile compounds. The results reveal possible mechanisms for the emission of strong fragrance by SF flowers, and provide a foundation for improvement of the floral fragrance and development of new crabapple cultivars. Frontiers Media S.A. 2023-01-20 /pmc/articles/PMC9895795/ /pubmed/36743501 http://dx.doi.org/10.3389/fpls.2023.1065219 Text en Copyright © 2023 Li, Liu, Zhang, Jia, Liu, Li, Zhou, Wang, Tan and Shao https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Li, Guofang
Liu, Jia
Zhang, He
Jia, Linguang
Liu, Youxian
Li, Jiuyang
Zhou, Shiwei
Wang, Pengjuan
Tan, Ming
Shao, Jianzhu
Volatile metabolome and floral transcriptome analyses reveal the volatile components of strongly fragrant progeny of Malus × robusta
title Volatile metabolome and floral transcriptome analyses reveal the volatile components of strongly fragrant progeny of Malus × robusta
title_full Volatile metabolome and floral transcriptome analyses reveal the volatile components of strongly fragrant progeny of Malus × robusta
title_fullStr Volatile metabolome and floral transcriptome analyses reveal the volatile components of strongly fragrant progeny of Malus × robusta
title_full_unstemmed Volatile metabolome and floral transcriptome analyses reveal the volatile components of strongly fragrant progeny of Malus × robusta
title_short Volatile metabolome and floral transcriptome analyses reveal the volatile components of strongly fragrant progeny of Malus × robusta
title_sort volatile metabolome and floral transcriptome analyses reveal the volatile components of strongly fragrant progeny of malus × robusta
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9895795/
https://www.ncbi.nlm.nih.gov/pubmed/36743501
http://dx.doi.org/10.3389/fpls.2023.1065219
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