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Transcriptome comparison analyses in UV-B induced AsA accumulation of Lactuca sativa L

BACKGROUND: Lettuce (Lactuca sativa L.) cultivated in facilities display low vitamin C (L-ascorbic acid (AsA)) contents which require augmentation. Although UV-B irradiation increases the accumulation of AsA in crops, processes underlying the biosynthesis as well as metabolism of AsA induced by UV-B...

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Autores principales: Zhou, Hua, Yu, Lei, Liu, Shujuan, Zhu, Anfan, Yang, Yanfang, Chen, Caihui, Yang, Aihong, Liu, Lipan, Yu, Faxin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9896689/
https://www.ncbi.nlm.nih.gov/pubmed/36737693
http://dx.doi.org/10.1186/s12864-023-09133-7
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author Zhou, Hua
Yu, Lei
Liu, Shujuan
Zhu, Anfan
Yang, Yanfang
Chen, Caihui
Yang, Aihong
Liu, Lipan
Yu, Faxin
author_facet Zhou, Hua
Yu, Lei
Liu, Shujuan
Zhu, Anfan
Yang, Yanfang
Chen, Caihui
Yang, Aihong
Liu, Lipan
Yu, Faxin
author_sort Zhou, Hua
collection PubMed
description BACKGROUND: Lettuce (Lactuca sativa L.) cultivated in facilities display low vitamin C (L-ascorbic acid (AsA)) contents which require augmentation. Although UV-B irradiation increases the accumulation of AsA in crops, processes underlying the biosynthesis as well as metabolism of AsA induced by UV-B in lettuce remain unclear. RESULTS: UV-B treatment increased the AsA content in lettuce, compared with that in the untreated control. UV-B treatment significantly increased AsA accumulation in a dose-dependent manner up until a certain dose.. Based on optimization experiments, three UV-B dose treatments, no UV-B (C), medium dose 7.2 KJ·m(− 2)·d(− 1) (U1), and high dose 12.96 KJ·m(− 2)·d(− 1) (U2), were selected for transcriptome sequencing (RNA-Seq) in this study. The results showed that C and U1 clustered in one category while U2 clustered in another, suggesting that the effect exerted on AsA by UV-B was dose dependent. MIOX gene in the myo-inositol pathway and APX gene in the recycling pathway in U2 were significantly different from the other two treatments, which was consistent with AsA changes seen in the three treatments, indicating that AsA accumulation caused by UV-B may be associated with these two genes in lettuce. UVR8 and HY5 were not significantly different expressed under UV-B irradiation, however, the genes involved in plant growth hormones and defence hormones significantly decreased and increased in U2, respectively, suggesting that high UV-B dose may regulate photomorphogenesis and response to stress via hormone regulatory pathways, although such regulation was independent of the UVR8 pathway. CONCLUSIONS: Our results demonstrated that studying the application of UV-B irradiation may enhance our understanding of the response of plant growth and AsA metabolism-related genes to UV-B stress, with particular reference to lettuce. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-023-09133-7.
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spelling pubmed-98966892023-02-04 Transcriptome comparison analyses in UV-B induced AsA accumulation of Lactuca sativa L Zhou, Hua Yu, Lei Liu, Shujuan Zhu, Anfan Yang, Yanfang Chen, Caihui Yang, Aihong Liu, Lipan Yu, Faxin BMC Genomics Research BACKGROUND: Lettuce (Lactuca sativa L.) cultivated in facilities display low vitamin C (L-ascorbic acid (AsA)) contents which require augmentation. Although UV-B irradiation increases the accumulation of AsA in crops, processes underlying the biosynthesis as well as metabolism of AsA induced by UV-B in lettuce remain unclear. RESULTS: UV-B treatment increased the AsA content in lettuce, compared with that in the untreated control. UV-B treatment significantly increased AsA accumulation in a dose-dependent manner up until a certain dose.. Based on optimization experiments, three UV-B dose treatments, no UV-B (C), medium dose 7.2 KJ·m(− 2)·d(− 1) (U1), and high dose 12.96 KJ·m(− 2)·d(− 1) (U2), were selected for transcriptome sequencing (RNA-Seq) in this study. The results showed that C and U1 clustered in one category while U2 clustered in another, suggesting that the effect exerted on AsA by UV-B was dose dependent. MIOX gene in the myo-inositol pathway and APX gene in the recycling pathway in U2 were significantly different from the other two treatments, which was consistent with AsA changes seen in the three treatments, indicating that AsA accumulation caused by UV-B may be associated with these two genes in lettuce. UVR8 and HY5 were not significantly different expressed under UV-B irradiation, however, the genes involved in plant growth hormones and defence hormones significantly decreased and increased in U2, respectively, suggesting that high UV-B dose may regulate photomorphogenesis and response to stress via hormone regulatory pathways, although such regulation was independent of the UVR8 pathway. CONCLUSIONS: Our results demonstrated that studying the application of UV-B irradiation may enhance our understanding of the response of plant growth and AsA metabolism-related genes to UV-B stress, with particular reference to lettuce. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-023-09133-7. BioMed Central 2023-02-03 /pmc/articles/PMC9896689/ /pubmed/36737693 http://dx.doi.org/10.1186/s12864-023-09133-7 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Zhou, Hua
Yu, Lei
Liu, Shujuan
Zhu, Anfan
Yang, Yanfang
Chen, Caihui
Yang, Aihong
Liu, Lipan
Yu, Faxin
Transcriptome comparison analyses in UV-B induced AsA accumulation of Lactuca sativa L
title Transcriptome comparison analyses in UV-B induced AsA accumulation of Lactuca sativa L
title_full Transcriptome comparison analyses in UV-B induced AsA accumulation of Lactuca sativa L
title_fullStr Transcriptome comparison analyses in UV-B induced AsA accumulation of Lactuca sativa L
title_full_unstemmed Transcriptome comparison analyses in UV-B induced AsA accumulation of Lactuca sativa L
title_short Transcriptome comparison analyses in UV-B induced AsA accumulation of Lactuca sativa L
title_sort transcriptome comparison analyses in uv-b induced asa accumulation of lactuca sativa l
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9896689/
https://www.ncbi.nlm.nih.gov/pubmed/36737693
http://dx.doi.org/10.1186/s12864-023-09133-7
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