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Immune cell-specific smoking-related expression characteristics are revealed by re-analysis of transcriptomes from the CEDAR cohort
INTRODUCTION: Smoking is known to affect whole-blood expression and methylation profiles. Although whole-genome methylation studies indicated that effects observed in blood may be driven by changes within leukocyte subtypes, these phenomena have not been explored using expression profiling. MATERIAL...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Termedia Publishing House
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9896985/ https://www.ncbi.nlm.nih.gov/pubmed/36817262 http://dx.doi.org/10.5114/ceji.2022.120618 |
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author | Nowak, Jan Krzysztof Dybska, Emilia Adams, Alex T. Walkowiak, Jaroslaw |
author_facet | Nowak, Jan Krzysztof Dybska, Emilia Adams, Alex T. Walkowiak, Jaroslaw |
author_sort | Nowak, Jan Krzysztof |
collection | PubMed |
description | INTRODUCTION: Smoking is known to affect whole-blood expression and methylation profiles. Although whole-genome methylation studies indicated that effects observed in blood may be driven by changes within leukocyte subtypes, these phenomena have not been explored using expression profiling. MATERIAL AND METHODS: This study reanalyzed data from the Correlated Expression and Disease Association Research (CEDAR) patient cohort recruited by Momozawa et al. (E-MTAB-6667). Data from gene expression profiling of immunomagnetically sorted CD4(+), CD8(+), CD14(+), CD15(+), and CD19(+) cells were processed. Differential expression analyses were conducted in each immune cell type, followed by gene ontology analysis and supplementary investigations. RESULTS: Ninety-four differentially expressed genes were found (CD8(+) n = 58, CD14(+) n = 20, CD4(+) n = 14, CD19(+) n = 2). Two key smoking-related genes were overexpressed in specific cell types: LRRN3 (CD4(+), CD8(+)) and MMP25 (CD8(+), CD14(+)). In CD4(+) cells smoking was associated with reduced expression of the NK cell receptor KLRB1, suggesting CD4(+) subpopulation shifts and differences in interferon signaling (reduced IRF1 and IL18RAP in smokers). Key results and their integration with an immune protein-protein interaction network revealed that smoking influences integrins in CD8(+) cells (ITGB7, ITGAL, ITGAM, ITGB2). C-type lectin CLEC4A was reduced in CD8(+) cells and CLEC10A was increased in CD14(+) cells from smokers; moreover, CLEC5A (CD8(+)), CLEC7A (CD8(+)) and CLEC9A (CD19(+)) were related to smoking in supplementary analyses. CD14(+) cells from smokers exhibited overexpression of LDLR and the formyl peptide receptor FPR3. CONCLUSIONS: Smoking specifically alters vital immune regulation genes in lymphocyte subtypes, especially CD4(+), CD8(+) and CD14(+) cells. |
format | Online Article Text |
id | pubmed-9896985 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Termedia Publishing House |
record_format | MEDLINE/PubMed |
spelling | pubmed-98969852023-02-16 Immune cell-specific smoking-related expression characteristics are revealed by re-analysis of transcriptomes from the CEDAR cohort Nowak, Jan Krzysztof Dybska, Emilia Adams, Alex T. Walkowiak, Jaroslaw Cent Eur J Immunol Experimental Immunology INTRODUCTION: Smoking is known to affect whole-blood expression and methylation profiles. Although whole-genome methylation studies indicated that effects observed in blood may be driven by changes within leukocyte subtypes, these phenomena have not been explored using expression profiling. MATERIAL AND METHODS: This study reanalyzed data from the Correlated Expression and Disease Association Research (CEDAR) patient cohort recruited by Momozawa et al. (E-MTAB-6667). Data from gene expression profiling of immunomagnetically sorted CD4(+), CD8(+), CD14(+), CD15(+), and CD19(+) cells were processed. Differential expression analyses were conducted in each immune cell type, followed by gene ontology analysis and supplementary investigations. RESULTS: Ninety-four differentially expressed genes were found (CD8(+) n = 58, CD14(+) n = 20, CD4(+) n = 14, CD19(+) n = 2). Two key smoking-related genes were overexpressed in specific cell types: LRRN3 (CD4(+), CD8(+)) and MMP25 (CD8(+), CD14(+)). In CD4(+) cells smoking was associated with reduced expression of the NK cell receptor KLRB1, suggesting CD4(+) subpopulation shifts and differences in interferon signaling (reduced IRF1 and IL18RAP in smokers). Key results and their integration with an immune protein-protein interaction network revealed that smoking influences integrins in CD8(+) cells (ITGB7, ITGAL, ITGAM, ITGB2). C-type lectin CLEC4A was reduced in CD8(+) cells and CLEC10A was increased in CD14(+) cells from smokers; moreover, CLEC5A (CD8(+)), CLEC7A (CD8(+)) and CLEC9A (CD19(+)) were related to smoking in supplementary analyses. CD14(+) cells from smokers exhibited overexpression of LDLR and the formyl peptide receptor FPR3. CONCLUSIONS: Smoking specifically alters vital immune regulation genes in lymphocyte subtypes, especially CD4(+), CD8(+) and CD14(+) cells. Termedia Publishing House 2022-11-16 2022 /pmc/articles/PMC9896985/ /pubmed/36817262 http://dx.doi.org/10.5114/ceji.2022.120618 Text en Copyright © 2022 Termedia https://creativecommons.org/licenses/by-nc-sa/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0). License (http://creativecommons.org/licenses/by-nc-sa/4.0/ (https://creativecommons.org/licenses/by-nc-sa/4.0/) ) |
spellingShingle | Experimental Immunology Nowak, Jan Krzysztof Dybska, Emilia Adams, Alex T. Walkowiak, Jaroslaw Immune cell-specific smoking-related expression characteristics are revealed by re-analysis of transcriptomes from the CEDAR cohort |
title | Immune cell-specific smoking-related expression characteristics are revealed by re-analysis of transcriptomes from the CEDAR cohort |
title_full | Immune cell-specific smoking-related expression characteristics are revealed by re-analysis of transcriptomes from the CEDAR cohort |
title_fullStr | Immune cell-specific smoking-related expression characteristics are revealed by re-analysis of transcriptomes from the CEDAR cohort |
title_full_unstemmed | Immune cell-specific smoking-related expression characteristics are revealed by re-analysis of transcriptomes from the CEDAR cohort |
title_short | Immune cell-specific smoking-related expression characteristics are revealed by re-analysis of transcriptomes from the CEDAR cohort |
title_sort | immune cell-specific smoking-related expression characteristics are revealed by re-analysis of transcriptomes from the cedar cohort |
topic | Experimental Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9896985/ https://www.ncbi.nlm.nih.gov/pubmed/36817262 http://dx.doi.org/10.5114/ceji.2022.120618 |
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