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Thermal tolerance of Cronobacter sakazakii and Cronobacter pulveris in reconstituted infant milk formula

BACKGROUND: Cronobacter sspecies are the most significant foodborne pathogen in infant milk formula (IMF). These pathogens have been incriminated in severe forms of neonatal meningitis, sepsis, and necrotizing enterocolitis with a high mortality rate. AIM: This study was performed to elucidate the e...

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Autores principales: Garbaj, Aboubaker M., Farag, Samira A., Sherif, Jihan A., Lawila, Aml F., Eshamah, Hanan L., Azwai, Salah M., Gammoudi, Fatim T., Naas, Hesham T., El Salabi, Allaaeddin A., Eldaghayes, Ibrahim M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Faculty of Veterinary Medicine 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9897503/
https://www.ncbi.nlm.nih.gov/pubmed/36777432
http://dx.doi.org/10.5455/OVJ.2023.v13.i1.11
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author Garbaj, Aboubaker M.
Farag, Samira A.
Sherif, Jihan A.
Lawila, Aml F.
Eshamah, Hanan L.
Azwai, Salah M.
Gammoudi, Fatim T.
Naas, Hesham T.
El Salabi, Allaaeddin A.
Eldaghayes, Ibrahim M.
author_facet Garbaj, Aboubaker M.
Farag, Samira A.
Sherif, Jihan A.
Lawila, Aml F.
Eshamah, Hanan L.
Azwai, Salah M.
Gammoudi, Fatim T.
Naas, Hesham T.
El Salabi, Allaaeddin A.
Eldaghayes, Ibrahim M.
author_sort Garbaj, Aboubaker M.
collection PubMed
description BACKGROUND: Cronobacter sspecies are the most significant foodborne pathogen in infant milk formula (IMF). These pathogens have been incriminated in severe forms of neonatal meningitis, sepsis, and necrotizing enterocolitis with a high mortality rate. AIM: This study was performed to elucidate the effect of heat stress on Cronobacter spp. (C. sakazakii and C. pulveris) in reconstituted IMF (RIMF). METHODS: The reconstituted formula was inoculated with five C. sakazakii isolates and four C. pulveris isolates separately. The nine isolates of Cronobacter spp. were heated in RIMF at 48°C, 52°C, 56°C, 60°C, 64°C, and 66°C. The D- and z-values were determined by using linear regression analysis. RESULTS: The D˗values of all isolates of C. sakazakii (CS1, CS3, CS4, CS5, and CS6) at 48°C, 52°C, 56°C, 60°C, 64°C, and 66°C were in the ranges 7.29–23.47, 2.77–15.50, 0.62–1.04, 0.62–1.02, 0.62–1.00, 0.62–1.00 minutes, respectively; while, the z˗values extended from 2.50°C to 4.28°C. The D˗ values of C. pulveris isolates (CP1, CP2, CP3, CP4) were in the ranges 7.60–22.32, 1.42–8.45, 0.62–1.08, 0.62–0.78, 0.62–0.78, 0.62–0.79 minutes at 48°C, 52°C, 56°C, 60°C, 64°C, 66°C, respectively and the calculated z-values ranged from 3.33°C to 4.89°C. CONCLUSION: This study may contribute to improving the understanding of the behavior of C. sakazakii and C. pulveris isolates in RIMF at various heat stress temperatures and may participate in the effective control of these pathogens in infant food production.
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spelling pubmed-98975032023-02-09 Thermal tolerance of Cronobacter sakazakii and Cronobacter pulveris in reconstituted infant milk formula Garbaj, Aboubaker M. Farag, Samira A. Sherif, Jihan A. Lawila, Aml F. Eshamah, Hanan L. Azwai, Salah M. Gammoudi, Fatim T. Naas, Hesham T. El Salabi, Allaaeddin A. Eldaghayes, Ibrahim M. Open Vet J Original Research BACKGROUND: Cronobacter sspecies are the most significant foodborne pathogen in infant milk formula (IMF). These pathogens have been incriminated in severe forms of neonatal meningitis, sepsis, and necrotizing enterocolitis with a high mortality rate. AIM: This study was performed to elucidate the effect of heat stress on Cronobacter spp. (C. sakazakii and C. pulveris) in reconstituted IMF (RIMF). METHODS: The reconstituted formula was inoculated with five C. sakazakii isolates and four C. pulveris isolates separately. The nine isolates of Cronobacter spp. were heated in RIMF at 48°C, 52°C, 56°C, 60°C, 64°C, and 66°C. The D- and z-values were determined by using linear regression analysis. RESULTS: The D˗values of all isolates of C. sakazakii (CS1, CS3, CS4, CS5, and CS6) at 48°C, 52°C, 56°C, 60°C, 64°C, and 66°C were in the ranges 7.29–23.47, 2.77–15.50, 0.62–1.04, 0.62–1.02, 0.62–1.00, 0.62–1.00 minutes, respectively; while, the z˗values extended from 2.50°C to 4.28°C. The D˗ values of C. pulveris isolates (CP1, CP2, CP3, CP4) were in the ranges 7.60–22.32, 1.42–8.45, 0.62–1.08, 0.62–0.78, 0.62–0.78, 0.62–0.79 minutes at 48°C, 52°C, 56°C, 60°C, 64°C, 66°C, respectively and the calculated z-values ranged from 3.33°C to 4.89°C. CONCLUSION: This study may contribute to improving the understanding of the behavior of C. sakazakii and C. pulveris isolates in RIMF at various heat stress temperatures and may participate in the effective control of these pathogens in infant food production. Faculty of Veterinary Medicine 2023-01 2023-01-21 /pmc/articles/PMC9897503/ /pubmed/36777432 http://dx.doi.org/10.5455/OVJ.2023.v13.i1.11 Text en https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Garbaj, Aboubaker M.
Farag, Samira A.
Sherif, Jihan A.
Lawila, Aml F.
Eshamah, Hanan L.
Azwai, Salah M.
Gammoudi, Fatim T.
Naas, Hesham T.
El Salabi, Allaaeddin A.
Eldaghayes, Ibrahim M.
Thermal tolerance of Cronobacter sakazakii and Cronobacter pulveris in reconstituted infant milk formula
title Thermal tolerance of Cronobacter sakazakii and Cronobacter pulveris in reconstituted infant milk formula
title_full Thermal tolerance of Cronobacter sakazakii and Cronobacter pulveris in reconstituted infant milk formula
title_fullStr Thermal tolerance of Cronobacter sakazakii and Cronobacter pulveris in reconstituted infant milk formula
title_full_unstemmed Thermal tolerance of Cronobacter sakazakii and Cronobacter pulveris in reconstituted infant milk formula
title_short Thermal tolerance of Cronobacter sakazakii and Cronobacter pulveris in reconstituted infant milk formula
title_sort thermal tolerance of cronobacter sakazakii and cronobacter pulveris in reconstituted infant milk formula
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9897503/
https://www.ncbi.nlm.nih.gov/pubmed/36777432
http://dx.doi.org/10.5455/OVJ.2023.v13.i1.11
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