Quantitative analysis of myofiber type composition in human and mouse skeletal muscles

Skeletal muscles are composed of different myofiber types characterized by the expression of myosin heavy chain isoforms, which can be affected by physical activity, aging, and pathological conditions. Here, we present a step-by-step high-throughput semi-automated approach for performing myofiber ty...

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Detalles Bibliográficos
Autores principales: Abbassi-Daloii, Tooba, el Abdellaoui, Salma, Kan, Hermien E., van den Akker, Erik, ’t Hoen, Peter A.C., Raz, Vered, Voortman, Lenard M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9898062/
https://www.ncbi.nlm.nih.gov/pubmed/36853713
http://dx.doi.org/10.1016/j.xpro.2023.102075
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author Abbassi-Daloii, Tooba
el Abdellaoui, Salma
Kan, Hermien E.
van den Akker, Erik
’t Hoen, Peter A.C.
Raz, Vered
Voortman, Lenard M.
author_facet Abbassi-Daloii, Tooba
el Abdellaoui, Salma
Kan, Hermien E.
van den Akker, Erik
’t Hoen, Peter A.C.
Raz, Vered
Voortman, Lenard M.
author_sort Abbassi-Daloii, Tooba
collection PubMed
description Skeletal muscles are composed of different myofiber types characterized by the expression of myosin heavy chain isoforms, which can be affected by physical activity, aging, and pathological conditions. Here, we present a step-by-step high-throughput semi-automated approach for performing myofiber type quantification of entire human or mouse muscle tissue sections, including immunofluorescence staining, image acquisition, processing, and quantification. For complete details on the use and execution of this protocol, please refer to Abbassi-Daloii et al. (2022).(1)
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spelling pubmed-98980622023-02-05 Quantitative analysis of myofiber type composition in human and mouse skeletal muscles Abbassi-Daloii, Tooba el Abdellaoui, Salma Kan, Hermien E. van den Akker, Erik ’t Hoen, Peter A.C. Raz, Vered Voortman, Lenard M. STAR Protoc Protocol Skeletal muscles are composed of different myofiber types characterized by the expression of myosin heavy chain isoforms, which can be affected by physical activity, aging, and pathological conditions. Here, we present a step-by-step high-throughput semi-automated approach for performing myofiber type quantification of entire human or mouse muscle tissue sections, including immunofluorescence staining, image acquisition, processing, and quantification. For complete details on the use and execution of this protocol, please refer to Abbassi-Daloii et al. (2022).(1) Elsevier 2023-01-29 /pmc/articles/PMC9898062/ /pubmed/36853713 http://dx.doi.org/10.1016/j.xpro.2023.102075 Text en © 2023 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Abbassi-Daloii, Tooba
el Abdellaoui, Salma
Kan, Hermien E.
van den Akker, Erik
’t Hoen, Peter A.C.
Raz, Vered
Voortman, Lenard M.
Quantitative analysis of myofiber type composition in human and mouse skeletal muscles
title Quantitative analysis of myofiber type composition in human and mouse skeletal muscles
title_full Quantitative analysis of myofiber type composition in human and mouse skeletal muscles
title_fullStr Quantitative analysis of myofiber type composition in human and mouse skeletal muscles
title_full_unstemmed Quantitative analysis of myofiber type composition in human and mouse skeletal muscles
title_short Quantitative analysis of myofiber type composition in human and mouse skeletal muscles
title_sort quantitative analysis of myofiber type composition in human and mouse skeletal muscles
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9898062/
https://www.ncbi.nlm.nih.gov/pubmed/36853713
http://dx.doi.org/10.1016/j.xpro.2023.102075
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