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HPLC analysis and in vitro antioxidant mediated through cell migration effect of C.hystrix water extract on human keratinocytes and fibroblasts

Citrus hystrix or kaffir lime is a native tropical plant containing a high level of phenolic and flavonoid compounds. Its fruits are used as a food ingredient to enhance the sour-sweet scent and flavor in many dishes. Due to its polyphenol-containing, it has also been used as traditional medicine fo...

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Autores principales: Ratanachamnong, Piyanee, Chunchaowarit, Yotsayut, Namchaiw, Poommaree, Niwaspragrit, Cholticha, Rattanacheeworn, Punyabhorn, Jaisin, Yamaratee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9898063/
https://www.ncbi.nlm.nih.gov/pubmed/36747533
http://dx.doi.org/10.1016/j.heliyon.2023.e13068
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author Ratanachamnong, Piyanee
Chunchaowarit, Yotsayut
Namchaiw, Poommaree
Niwaspragrit, Cholticha
Rattanacheeworn, Punyabhorn
Jaisin, Yamaratee
author_facet Ratanachamnong, Piyanee
Chunchaowarit, Yotsayut
Namchaiw, Poommaree
Niwaspragrit, Cholticha
Rattanacheeworn, Punyabhorn
Jaisin, Yamaratee
author_sort Ratanachamnong, Piyanee
collection PubMed
description Citrus hystrix or kaffir lime is a native tropical plant containing a high level of phenolic and flavonoid compounds. Its fruits are used as a food ingredient to enhance the sour-sweet scent and flavor in many dishes. Due to its polyphenol-containing, it has also been used as traditional medicine for health benefits including oral and gum health, stress relief, hair care, and skincare. In this study, we demonstrated the antioxidant activity of C. hystrix water extract and its effect on human keratinocyte and fibroblast migration. The extract showed a high amount of phenolic and flavonoid contents. The HPLC analysis indicated the presence of gallic acid, catechin, caffeic acid, rutin, and quercetin. We showed that C. hystrix water extract exhibited free radical scavenging capacity, determined by DPPH assay, with IC(50) of 14.91 mg/mL, and nitrite radical scavenging capacity, determined by NO assay, with IC(50) of 4.46 mg/mL. The C. hystrix water extract displayed unnoticeable toxicity at all tested doses. We showed that the treatment of water extracts as low as 50 μg/mL decreased the reactive oxygen species (ROS) from H(2)O(2)-induced ROS formation in both cell lines. Besides, C. hystrix water extract promoted cell migration in a dose-dependent manner. Together, these results demonstrated the positive benefit of C. hystrix water extract as a wound-healing accelerator. Its health benefits may be due to the antioxidant capability of its phytochemical compounds contained in C. hystrix water extract that enhances the migration of two major cell types: fibroblast and keratinocytes, responsible for the proliferation and remodeling phase of wound healing.
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spelling pubmed-98980632023-02-05 HPLC analysis and in vitro antioxidant mediated through cell migration effect of C.hystrix water extract on human keratinocytes and fibroblasts Ratanachamnong, Piyanee Chunchaowarit, Yotsayut Namchaiw, Poommaree Niwaspragrit, Cholticha Rattanacheeworn, Punyabhorn Jaisin, Yamaratee Heliyon Research Article Citrus hystrix or kaffir lime is a native tropical plant containing a high level of phenolic and flavonoid compounds. Its fruits are used as a food ingredient to enhance the sour-sweet scent and flavor in many dishes. Due to its polyphenol-containing, it has also been used as traditional medicine for health benefits including oral and gum health, stress relief, hair care, and skincare. In this study, we demonstrated the antioxidant activity of C. hystrix water extract and its effect on human keratinocyte and fibroblast migration. The extract showed a high amount of phenolic and flavonoid contents. The HPLC analysis indicated the presence of gallic acid, catechin, caffeic acid, rutin, and quercetin. We showed that C. hystrix water extract exhibited free radical scavenging capacity, determined by DPPH assay, with IC(50) of 14.91 mg/mL, and nitrite radical scavenging capacity, determined by NO assay, with IC(50) of 4.46 mg/mL. The C. hystrix water extract displayed unnoticeable toxicity at all tested doses. We showed that the treatment of water extracts as low as 50 μg/mL decreased the reactive oxygen species (ROS) from H(2)O(2)-induced ROS formation in both cell lines. Besides, C. hystrix water extract promoted cell migration in a dose-dependent manner. Together, these results demonstrated the positive benefit of C. hystrix water extract as a wound-healing accelerator. Its health benefits may be due to the antioxidant capability of its phytochemical compounds contained in C. hystrix water extract that enhances the migration of two major cell types: fibroblast and keratinocytes, responsible for the proliferation and remodeling phase of wound healing. Elsevier 2023-01-24 /pmc/articles/PMC9898063/ /pubmed/36747533 http://dx.doi.org/10.1016/j.heliyon.2023.e13068 Text en © 2023 The Authors. Published by Elsevier Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Ratanachamnong, Piyanee
Chunchaowarit, Yotsayut
Namchaiw, Poommaree
Niwaspragrit, Cholticha
Rattanacheeworn, Punyabhorn
Jaisin, Yamaratee
HPLC analysis and in vitro antioxidant mediated through cell migration effect of C.hystrix water extract on human keratinocytes and fibroblasts
title HPLC analysis and in vitro antioxidant mediated through cell migration effect of C.hystrix water extract on human keratinocytes and fibroblasts
title_full HPLC analysis and in vitro antioxidant mediated through cell migration effect of C.hystrix water extract on human keratinocytes and fibroblasts
title_fullStr HPLC analysis and in vitro antioxidant mediated through cell migration effect of C.hystrix water extract on human keratinocytes and fibroblasts
title_full_unstemmed HPLC analysis and in vitro antioxidant mediated through cell migration effect of C.hystrix water extract on human keratinocytes and fibroblasts
title_short HPLC analysis and in vitro antioxidant mediated through cell migration effect of C.hystrix water extract on human keratinocytes and fibroblasts
title_sort hplc analysis and in vitro antioxidant mediated through cell migration effect of c.hystrix water extract on human keratinocytes and fibroblasts
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9898063/
https://www.ncbi.nlm.nih.gov/pubmed/36747533
http://dx.doi.org/10.1016/j.heliyon.2023.e13068
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