Cargando…

Enhanced bacterial clearance in early secondary sepsis in a porcine intensive care model

Early secondary sepsis (ESS), occurring after recent inflammatory activation is associated with a reduced inflammatory response. If this attenuation also is associated with decreased bacterial killing, the need for antibiotic efficacy might be greater than in primary sepsis (PS). This prospective, r...

Descripción completa

Detalles Bibliográficos
Autores principales: Wilske, Frida, Skorup, Paul, Hanslin, Katja, Janols, Helena, Larsson, Anders, Lipcsey, Miklós, Sjölin, Jan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9898276/
https://www.ncbi.nlm.nih.gov/pubmed/36737631
http://dx.doi.org/10.1038/s41598-023-28880-x
_version_ 1784882392083726336
author Wilske, Frida
Skorup, Paul
Hanslin, Katja
Janols, Helena
Larsson, Anders
Lipcsey, Miklós
Sjölin, Jan
author_facet Wilske, Frida
Skorup, Paul
Hanslin, Katja
Janols, Helena
Larsson, Anders
Lipcsey, Miklós
Sjölin, Jan
author_sort Wilske, Frida
collection PubMed
description Early secondary sepsis (ESS), occurring after recent inflammatory activation is associated with a reduced inflammatory response. If this attenuation also is associated with decreased bacterial killing, the need for antibiotic efficacy might be greater than in primary sepsis (PS). This prospective, randomised interventional study compares bacterial killing in ESS and PS in a large animal intensive care sepsis model. 38 pigs were intravenously administered live Escherichia coli for 3 h. Before baseline ESS was pre-exposed to endotoxin 24 h, whereas PS was not. Bacterial growth was measured in organs immediately post-mortem, repeatedly during 6 h in blood in vivo and for blood intrinsic bactericidal capacity ex vivo. Splenic growth was lower in ESS animals, than in PS animals (3.31 ± 0.12, vs. 3.84 ± 0.14 log(10) CFU/mL, mean ± SEM) (p < 0.01) with a similar trend in hepatic growth (p = NS). Blood bacterial count at 2 h correlated with splenic bacterial count in ESS (ESS: r = 0.71, p < 0.001) and to blood killing capacity in PS (PS: r = 0.69, p < 0.001). Attenuated inflammation in ESS is associated with enhanced antibacterial capacities in the spleen. In ESS blood bacterial count is related to splenic killing and in PS to blood bactericidal capacity. The results suggest no increased need for synergistic antibiotic combinations in ESS.
format Online
Article
Text
id pubmed-9898276
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-98982762023-02-05 Enhanced bacterial clearance in early secondary sepsis in a porcine intensive care model Wilske, Frida Skorup, Paul Hanslin, Katja Janols, Helena Larsson, Anders Lipcsey, Miklós Sjölin, Jan Sci Rep Article Early secondary sepsis (ESS), occurring after recent inflammatory activation is associated with a reduced inflammatory response. If this attenuation also is associated with decreased bacterial killing, the need for antibiotic efficacy might be greater than in primary sepsis (PS). This prospective, randomised interventional study compares bacterial killing in ESS and PS in a large animal intensive care sepsis model. 38 pigs were intravenously administered live Escherichia coli for 3 h. Before baseline ESS was pre-exposed to endotoxin 24 h, whereas PS was not. Bacterial growth was measured in organs immediately post-mortem, repeatedly during 6 h in blood in vivo and for blood intrinsic bactericidal capacity ex vivo. Splenic growth was lower in ESS animals, than in PS animals (3.31 ± 0.12, vs. 3.84 ± 0.14 log(10) CFU/mL, mean ± SEM) (p < 0.01) with a similar trend in hepatic growth (p = NS). Blood bacterial count at 2 h correlated with splenic bacterial count in ESS (ESS: r = 0.71, p < 0.001) and to blood killing capacity in PS (PS: r = 0.69, p < 0.001). Attenuated inflammation in ESS is associated with enhanced antibacterial capacities in the spleen. In ESS blood bacterial count is related to splenic killing and in PS to blood bactericidal capacity. The results suggest no increased need for synergistic antibiotic combinations in ESS. Nature Publishing Group UK 2023-02-03 /pmc/articles/PMC9898276/ /pubmed/36737631 http://dx.doi.org/10.1038/s41598-023-28880-x Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Wilske, Frida
Skorup, Paul
Hanslin, Katja
Janols, Helena
Larsson, Anders
Lipcsey, Miklós
Sjölin, Jan
Enhanced bacterial clearance in early secondary sepsis in a porcine intensive care model
title Enhanced bacterial clearance in early secondary sepsis in a porcine intensive care model
title_full Enhanced bacterial clearance in early secondary sepsis in a porcine intensive care model
title_fullStr Enhanced bacterial clearance in early secondary sepsis in a porcine intensive care model
title_full_unstemmed Enhanced bacterial clearance in early secondary sepsis in a porcine intensive care model
title_short Enhanced bacterial clearance in early secondary sepsis in a porcine intensive care model
title_sort enhanced bacterial clearance in early secondary sepsis in a porcine intensive care model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9898276/
https://www.ncbi.nlm.nih.gov/pubmed/36737631
http://dx.doi.org/10.1038/s41598-023-28880-x
work_keys_str_mv AT wilskefrida enhancedbacterialclearanceinearlysecondarysepsisinaporcineintensivecaremodel
AT skoruppaul enhancedbacterialclearanceinearlysecondarysepsisinaporcineintensivecaremodel
AT hanslinkatja enhancedbacterialclearanceinearlysecondarysepsisinaporcineintensivecaremodel
AT janolshelena enhancedbacterialclearanceinearlysecondarysepsisinaporcineintensivecaremodel
AT larssonanders enhancedbacterialclearanceinearlysecondarysepsisinaporcineintensivecaremodel
AT lipcseymiklos enhancedbacterialclearanceinearlysecondarysepsisinaporcineintensivecaremodel
AT sjolinjan enhancedbacterialclearanceinearlysecondarysepsisinaporcineintensivecaremodel