Protocol for cell-type-specific tissue reconstruction in the murine lung fibrogenic microenvironment

Pulmonary fibrosis is a process characterized by epithelial injury and fibroblast activation. It is also well recognized as a predisposition to lung cancer. Here, we present a protocol to establish an in vivo model to evaluate the dynamics of alveolar epithelial type 2 cells and lung cancer cells in...

Descripción completa

Detalles Bibliográficos
Autores principales: Hasegawa, Koichi, Miyata, Ryo, Menju, Toshi, Hirai, Toyohiro, Date, Hiroshi, Sato, Atsuyasu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9898780/
https://www.ncbi.nlm.nih.gov/pubmed/36853706
http://dx.doi.org/10.1016/j.xpro.2022.102018
_version_ 1784882502528139264
author Hasegawa, Koichi
Miyata, Ryo
Menju, Toshi
Hirai, Toyohiro
Date, Hiroshi
Sato, Atsuyasu
author_facet Hasegawa, Koichi
Miyata, Ryo
Menju, Toshi
Hirai, Toyohiro
Date, Hiroshi
Sato, Atsuyasu
author_sort Hasegawa, Koichi
collection PubMed
description Pulmonary fibrosis is a process characterized by epithelial injury and fibroblast activation. It is also well recognized as a predisposition to lung cancer. Here, we present a protocol to establish an in vivo model to evaluate the dynamics of alveolar epithelial type 2 cells and lung cancer cells in the context of the lung fibrogenic microenvironment. Utilizing the cell transfer technique, we detail a basis for therapeutic approaches in pulmonary fibrosis and tools for precision medicine against lung cancer. For complete details on the use and execution of this protocol, please refer to Miyata et al. (2022).(1)
format Online
Article
Text
id pubmed-9898780
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-98987802023-02-05 Protocol for cell-type-specific tissue reconstruction in the murine lung fibrogenic microenvironment Hasegawa, Koichi Miyata, Ryo Menju, Toshi Hirai, Toyohiro Date, Hiroshi Sato, Atsuyasu STAR Protoc Protocol Pulmonary fibrosis is a process characterized by epithelial injury and fibroblast activation. It is also well recognized as a predisposition to lung cancer. Here, we present a protocol to establish an in vivo model to evaluate the dynamics of alveolar epithelial type 2 cells and lung cancer cells in the context of the lung fibrogenic microenvironment. Utilizing the cell transfer technique, we detail a basis for therapeutic approaches in pulmonary fibrosis and tools for precision medicine against lung cancer. For complete details on the use and execution of this protocol, please refer to Miyata et al. (2022).(1) Elsevier 2023-01-25 /pmc/articles/PMC9898780/ /pubmed/36853706 http://dx.doi.org/10.1016/j.xpro.2022.102018 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Hasegawa, Koichi
Miyata, Ryo
Menju, Toshi
Hirai, Toyohiro
Date, Hiroshi
Sato, Atsuyasu
Protocol for cell-type-specific tissue reconstruction in the murine lung fibrogenic microenvironment
title Protocol for cell-type-specific tissue reconstruction in the murine lung fibrogenic microenvironment
title_full Protocol for cell-type-specific tissue reconstruction in the murine lung fibrogenic microenvironment
title_fullStr Protocol for cell-type-specific tissue reconstruction in the murine lung fibrogenic microenvironment
title_full_unstemmed Protocol for cell-type-specific tissue reconstruction in the murine lung fibrogenic microenvironment
title_short Protocol for cell-type-specific tissue reconstruction in the murine lung fibrogenic microenvironment
title_sort protocol for cell-type-specific tissue reconstruction in the murine lung fibrogenic microenvironment
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9898780/
https://www.ncbi.nlm.nih.gov/pubmed/36853706
http://dx.doi.org/10.1016/j.xpro.2022.102018
work_keys_str_mv AT hasegawakoichi protocolforcelltypespecifictissuereconstructioninthemurinelungfibrogenicmicroenvironment
AT miyataryo protocolforcelltypespecifictissuereconstructioninthemurinelungfibrogenicmicroenvironment
AT menjutoshi protocolforcelltypespecifictissuereconstructioninthemurinelungfibrogenicmicroenvironment
AT hiraitoyohiro protocolforcelltypespecifictissuereconstructioninthemurinelungfibrogenicmicroenvironment
AT datehiroshi protocolforcelltypespecifictissuereconstructioninthemurinelungfibrogenicmicroenvironment
AT satoatsuyasu protocolforcelltypespecifictissuereconstructioninthemurinelungfibrogenicmicroenvironment

Ejemplares similares