Rapid yeast-based screen for Functionally Relevant Amino Acids (RS-FRAA) in a protein

Here, we describe a fast and cost-effective procedure to generate a large array of mutant proteins and immediately screen for those with altered protein function. This protocol is a modification from three existing approaches: fusion PCR, Saccharomyces cerevisiae in-yeast recombination, and semi-qua...

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Detalles Bibliográficos
Autores principales: Ghuge, Aditi A., Anderson, Reuben A., Gottfried, Susanne, Daube, Clément, Koloamatangi, Siaosi M.B.M.J., Schiemann, Anja H., Sattlegger, Evelyn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9898782/
https://www.ncbi.nlm.nih.gov/pubmed/36856772
http://dx.doi.org/10.1016/j.xpro.2022.101545

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9898782/
https://www.ncbi.nlm.nih.gov/pubmed/36856772
http://dx.doi.org/10.1016/j.xpro.2022.101545

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