Fast and efficient synaptosome isolation and post-synaptic density enrichment from hiPSC-motor neurons by biochemical sub-cellular fractionation

We describe here a time-efficient, in-house protocol for synaptosome isolation and enrichment of the post-synaptic density (PSD) from hiPSC-derived motor neurons. By using biochemical sub-cellular fractionation, the crude synaptosome is first isolated from the cytosol and is then further separated i...

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Detalles Bibliográficos
Autores principales: Rajkumar, Sandeep, Böckers, Tobias M., Catanese, Alberto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9898788/
https://www.ncbi.nlm.nih.gov/pubmed/36853677
http://dx.doi.org/10.1016/j.xpro.2023.102061
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author Rajkumar, Sandeep
Böckers, Tobias M.
Catanese, Alberto
author_facet Rajkumar, Sandeep
Böckers, Tobias M.
Catanese, Alberto
author_sort Rajkumar, Sandeep
collection PubMed
description We describe here a time-efficient, in-house protocol for synaptosome isolation and enrichment of the post-synaptic density (PSD) from hiPSC-derived motor neurons. By using biochemical sub-cellular fractionation, the crude synaptosome is first isolated from the cytosol and is then further separated into the synaptic cytosol and the enriched PSD fraction. The protocol can also potentially be adapted to other hiPSC-derived neuronal types, with necessary changes made to cell seeding density and buffer volumes.
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spelling pubmed-98987882023-02-05 Fast and efficient synaptosome isolation and post-synaptic density enrichment from hiPSC-motor neurons by biochemical sub-cellular fractionation Rajkumar, Sandeep Böckers, Tobias M. Catanese, Alberto STAR Protoc Protocol We describe here a time-efficient, in-house protocol for synaptosome isolation and enrichment of the post-synaptic density (PSD) from hiPSC-derived motor neurons. By using biochemical sub-cellular fractionation, the crude synaptosome is first isolated from the cytosol and is then further separated into the synaptic cytosol and the enriched PSD fraction. The protocol can also potentially be adapted to other hiPSC-derived neuronal types, with necessary changes made to cell seeding density and buffer volumes. Elsevier 2023-01-25 /pmc/articles/PMC9898788/ /pubmed/36853677 http://dx.doi.org/10.1016/j.xpro.2023.102061 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Rajkumar, Sandeep
Böckers, Tobias M.
Catanese, Alberto
Fast and efficient synaptosome isolation and post-synaptic density enrichment from hiPSC-motor neurons by biochemical sub-cellular fractionation
title Fast and efficient synaptosome isolation and post-synaptic density enrichment from hiPSC-motor neurons by biochemical sub-cellular fractionation
title_full Fast and efficient synaptosome isolation and post-synaptic density enrichment from hiPSC-motor neurons by biochemical sub-cellular fractionation
title_fullStr Fast and efficient synaptosome isolation and post-synaptic density enrichment from hiPSC-motor neurons by biochemical sub-cellular fractionation
title_full_unstemmed Fast and efficient synaptosome isolation and post-synaptic density enrichment from hiPSC-motor neurons by biochemical sub-cellular fractionation
title_short Fast and efficient synaptosome isolation and post-synaptic density enrichment from hiPSC-motor neurons by biochemical sub-cellular fractionation
title_sort fast and efficient synaptosome isolation and post-synaptic density enrichment from hipsc-motor neurons by biochemical sub-cellular fractionation
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9898788/
https://www.ncbi.nlm.nih.gov/pubmed/36853677
http://dx.doi.org/10.1016/j.xpro.2023.102061
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