Fast and efficient synaptosome isolation and post-synaptic density enrichment from hiPSC-motor neurons by biochemical sub-cellular fractionation

We describe here a time-efficient, in-house protocol for synaptosome isolation and enrichment of the post-synaptic density (PSD) from hiPSC-derived motor neurons. By using biochemical sub-cellular fractionation, the crude synaptosome is first isolated from the cytosol and is then further separated i...

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Detalles Bibliográficos
Autores principales: Rajkumar, Sandeep, Böckers, Tobias M., Catanese, Alberto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9898788/
https://www.ncbi.nlm.nih.gov/pubmed/36853677
http://dx.doi.org/10.1016/j.xpro.2023.102061

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9898788/
https://www.ncbi.nlm.nih.gov/pubmed/36853677
http://dx.doi.org/10.1016/j.xpro.2023.102061

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