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MERR APEX-seq protocol for profiling the subcellular nascent transcriptome in mammalian cells

Knowledge about the spatial organization of RNAs in eukaryotic cells is crucial for understanding their functions. Here, we present a detailed MERR APEX-seq protocol to achieve spatiotemporally resolved mapping of the subcellular transcriptome in cultured mammalian cells. This protocol provides deta...

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Detalles Bibliográficos
Autores principales: Li, Ran, Zou, Peng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9898802/
https://www.ncbi.nlm.nih.gov/pubmed/36853684
http://dx.doi.org/10.1016/j.xpro.2023.102057