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MERR APEX-seq protocol for profiling the subcellular nascent transcriptome in mammalian cells
Knowledge about the spatial organization of RNAs in eukaryotic cells is crucial for understanding their functions. Here, we present a detailed MERR APEX-seq protocol to achieve spatiotemporally resolved mapping of the subcellular transcriptome in cultured mammalian cells. This protocol provides deta...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2023
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9898802/ https://www.ncbi.nlm.nih.gov/pubmed/36853684 http://dx.doi.org/10.1016/j.xpro.2023.102057 |