Protocol for analyzing enzymatic hydrolysis of cellulose using surface pitting observation technology

Assaying enzymatic degradation of water-insoluble substrates like cellulose is challenging because only the substrate surface is accessible to the enzymes resulting in low reaction rates. Here, we describe a protocol for surface pitting observation technology (SPOT), an ultra-sensitive quantitative...

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Detalles Bibliográficos
Autores principales: Tachioka, Mikako, Tsudome, Mikiko, Deguchi, Shigeru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9898803/
https://www.ncbi.nlm.nih.gov/pubmed/36825811
http://dx.doi.org/10.1016/j.xpro.2023.102066

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